Cytofluorometric analysis of anti-lymphocyte antibodies in AIDS. NLM AIDSLINE Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

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Cytofluorometric analysis of anti-lymphocyte antibodies in AIDS.

FEMS Microbiol Immunol. 1991 Dec;4(1):33-40. Unique Identifier : AIDSLINE MED/92273231
Bonner BC; Poulton TA; Department of Pathology, University of Dundee, Ninewells Hospital; Medical School, U.K.

Abstract: Anti-lymphocyte antibodies (ALA) have been detected in the plasma of 53.8% of HIV-positive patients tested (CD4/CD8 ratios: mean 0.265; range 0.01 to 0.5) using analytical continuous-flow cytofluorometry. IgG from the AIDS plasma was seen to bind to normal PBL in 53.8% of cases (14/26). In double labelling experiments CD4 + lymphocytes, CD8 + lymphocytes, and B lymphocytes were all bound by the ALA, but monocytes were not bound. Pre-adsorption of the diluted AIDS plasma onto an excess of mouse spleen cells did not remove lymphocyte binding activity. No evidence was found for preferential binding to phytohaemagglutinin-stimulated lymphocytes. ALA could not be detected in the plasma of normal subjects, patients with acute renal failure undergoing renal dialysis, or patients with high levels of circulating immune complexes.
Keywords: Acquired Immunodeficiency Syndrome/*IMMUNOLOGY Antilymphocyte Serum/*IMMUNOLOGY Flow Cytometry Human IgG/ANALYSIS Immunoglobulins, Surface/ANALYSIS Lymphocyte Subsets/IMMUNOLOGY Phytohemagglutinins/IMMUNOLOGY Sialoglycoproteins/ANALYSIS Support, Non-U.S. Gov't JOURNAL ARTICLEKWDacquiredimmunodeficiencysyndrome/KWDimmunologyantilymphocyteserum/KWDimmunologyflowcytometryhumanigg/analysisimmunoglobulins,surface/analysislymphocytesubsets/immunologyphytohemagglutinins/immunologysialoglycoproteins/analysissupport,non-uKWDsKWDgov'tjournalarticle
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M9290896

Copyright © 1992 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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