Immunochemical and functional characterization of anti-idiotypic antibodies to a mouse anti-CD4 monoclonal antibody.

Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

Immunochemical and functional characterization of anti-idiotypic antibodies to a mouse anti-CD4 monoclonal antibody.

Int J Clin Lab Res. 1991;21(2):179-85. Unique Identifier : AIDSLINE MED/92273764
Perosa F; Dannecker G; Ferrone S; Dammacco F; Department of Biomedical Sciences and Human Oncology, University; of Bari Medical School, Italy.

Abstract: Immunization of BALB/c mice with the mouse anti-CD4 monoclonal antibody (mAb) HP2/6 resulted in the production of anti-idiotypic antibodies. Analysis of the kinetics of the development of anti-idiotypic antibodies showed a homogeneous response among the immunized animals. Cross-blocking assays performed with anti-CD4 mAbs OKT4, OKT4c and OKT4d showed that syngeneic anti-idiotypic antiserum elicited with mAb HP2/6 recognizes idiotope(s) expressed only on the immunizing mAb. The idiotope(s) is (are) located within or closely related to the antigen-combining site of mAb HP2/6. Hybridization with the myeloma cell line NSO of splenocytes from a BALB/c mouse hyperimmunized with mAb HP2/6 generated the anti-idiotypic mAbs F11-2113, F11-2302 and F11-2444 which recognize idiotope(s) outside the antigen-combining site of mAb HP2/6. Although the anti-idiotypic mAbs cross-inhibit each other in their binding to mAb HP2/6, they differ in the ability to elicit anti-anti-idiotypic antisera. Furthermore, mAb F11-2113 enhances CD4 down-regulation in the presence of mAb HP2/6 to a larger extent than mAbs F11-2302 and F11-2444. The latter results suggest an additional mechanism by which anti-idiotypic antibodies may induce functional abnormalities of CD4+ T cells in human immunodeficiency virus-infected T cells.

Keywords: Animal Antibodies, Anti-Idiotypic/*IMMUNOLOGY Antibodies, Monoclonal/*IMMUNOLOGY Antibody Specificity Antigens, CD4/*IMMUNOLOGY Human Immune Sera Immunization Immunoglobulin Idiotypes/*IMMUNOLOGY Immunoglobulins, Fab/IMMUNOLOGY Kinetics Mice Mice, Inbred BALB C/IMMUNOLOGY Support, Non-U.S. Gov't JOURNAL ARTICLE
920930
M9290892

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