Identification and isolation of Cryptosporidium parvum genes encoding microtubule and microfilament proteins.

Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

Identification and isolation of Cryptosporidium parvum genes encoding microtubule and microfilament proteins.

J Protozool. 1991 Nov-Dec;38(6):52S-55S. Unique Identifier : AIDSLINE MED/92292035
Nelson RG; Kim K; Gooze L; Petersen C; Gut J; Parasitology Laboratory, Department of Medicine, San Francisco; General Hospital, University of California 94143.

Abstract: Microtubules and microfilaments are highly conserved cytoskeletal polymers hypothesized to play essential biomechanical roles in the unusual gliding motility of Apicomplexan zoites and in their invasion of, and development within, host epithelial cells. We have identified and isolated Cryptosporidium parvum genes encoding the microtubule proteins alpha- and beta-tubulin and the microfilament protein actin by screening a lambda gt11 C. parvum genomic DNA library with degenerate oligonucleotide and heterologous cDNA hybridization probes respectively. The alpha- and beta-tubulin genes have been partially sequenced and the deduced peptide sequences show greatest homology with the tubulins of the related parasites, T. gondii and P. falciparum. The complete nucleic acid sequence of the actin gene predicts a 376 amino acid, 42 kDa protein having 85% sequence identity with the P. falciparum actin I and the human gamma-actin proteins. Each of these cytoskeletal protein genes was demonstrated to be of cryptosporidial origin by Southern analyses of C. parvum chromosomes fractionated by pulsed field gel electrophoresis; the cloned alpha- and beta-tubulin genes hybridized with chromosomes of ca. 1,200 and 1,500 kb respectively and the cloned actin gene also hybridized with a 1,200 kb chromosome.

Keywords: Acquired Immunodeficiency Syndrome/PARASITOLOGY Actins/*GENETICS Amino Acid Sequence Animal Cattle Cryptosporidium parvum/*GENETICS DNA, Protozoan Genomic Library Human Molecular Sequence Data Protozoan Proteins/*GENETICS Sequence Alignment Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Tubulin/*GENETICS JOURNAL ARTICLE
920930
M9290845

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1992. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1992. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .