In vitro biosynthesis of [Thr2, Leu5, D-Hiv8, Leu10]cyclosporin, a cyclosporin-related immunosuppressive peptolide. NLM AIDSLINE Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

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In vitro biosynthesis of [Thr2, Leu5, D-Hiv8, Leu10]cyclosporin, a cyclosporin-related immunosuppressive peptolide.

Biomed Biochim Acta. 1991;50(10-11):S260-3. Unique Identifier : AIDSLINE MED/92313349
Lawen A; Traber R; Geyl D; Technical University Berlin, Institute of Biochemistry and; Molecular Biology, F.R.G.

Abstract: We were able to prepare an enzyme fraction from crude extracts of the mycelium of the fungus Cylindrotrichum Bonorden, which is capable of synthesizing the new peptolide SDZ 214-103 under consumption of the constitutive amino acids, D-2-hydroxyisovaleric acid, ATP and S-adenosyl-L-methionine. The enzyme does not synthesize CyA, while cyclosporin synthetase does not synthesize the peptolide. Peptolide synthetase has a high molecular weight in the same range as cyclosporin synthetase (about 1.5 MDa).
Keywords: Amino Acid Sequence Cyclosporins/*BIOSYNTHESIS/CHEMISTRY Hyphomycetes/METABOLISM Immunosuppressive Agents/CHEMISTRY Molecular Sequence Data Molecular Structure Molecular Weight Peptide Synthetases/ISOLATION & PURIF/METABOLISM JOURNAL ARTICLEKWDaminoacidsequencecyclosporins/KWDbiosynthesis/chemistryhyphomycetes/metabolismimmunosuppressiveagents/chemistrymolecularsequencedatamolecularstructuremolecularweightpeptidesynthetases/isolation&purif/metabolismjournalarticle
921030
M92A1001

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