[Analogs of nucleotides, modified by a sugar residue and pyrimidine base, in a DNA synthesis reaction, catalyzed by Thermus aquaticus DNA polymerase] NLM AIDSLINE Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

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[Analogs of nucleotides, modified by a sugar residue and pyrimidine base, in a DNA synthesis reaction, catalyzed by Thermus aquaticus DNA polymerase]

Mol Biol (Mosk). 1992 Jan-Feb;26(1):191-200. Unique Identifier : AIDSLINE MED/92375024
Savochkina LP; Diachenko LB; Lukin MA; Aleksandrova LA

Abstract: Substrate properties of dNTP analogues in the DNA synthesis reaction catalyzed by Thermus aquaticus DNA polymerase were studied. It was shown that most of dNTP analogues which were known as terminators of DNA synthesis of E. coli DNA polymerase I were able to terminate DNA synthesis catalyzed by Thermus aquaticus DNA polymerase. An interesting feature of Thermus aquaticus DNA polymerase was the ability to utilize 3'-azido-2',3'-dideoxythymidine triphosphate as terminating substrate. Relative efficiency of tested dNTP analogues incorporation into the DNA growing chain was estimated.
Keywords: Base Sequence Carbohydrates/*CHEMISTRY Catalysis Cell-Free System DNA/*BIOSYNTHESIS DNA Polymerases/*METABOLISM English Abstract Molecular Sequence Data Nucleotides/*CHEMISTRY Pyrimidines/*CHEMISTRY Substrate Specificity Thermus/*ENZYMOLOGY *Thymine Nucleotides Zidovudine/*ANALOGS & DERIVATIVES JOURNAL ARTICLE

KWDbasesequencecarbohydrates/KWDchemistrycatalysiscell-freesystemdna/KWDbiosynthesisdnapolymerases/KWDmetabolismenglishabstractmolecularsequencedatanucleotides/KWDchemistrypyrimidines/KWDchemistrysubstratespecificitythermus/KWDenzymologyKWDthyminenucleotideszidovudine/KWDanalogs&derivativesjournalarticle
921130
M92B0324

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