Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
MOLECULAR DETECTION OF EPSTEIN-BARR VIRUS IN TISSUE (MEETING ABSTRACT)
Causes, Cures and Consequences of Lymphoproliferative Diseases--A Symposium to Honor George and Eva Klein. May 9-11, 1991, Omaha, NE, 1991.. Unique Identifier : AIDSLINE ICDB/92679937 Gorm P; Hamilton-Dutoit S; Sandvej K; Lab. of Immunohistology, Aarhus University Hosp.,; Kommunehospitalet, 8000 Aarhus C, Denmark
Abstract:
EBV infection in humans is associated epithelial lesions (nasopharyngeal carcinoma, histologically similar carcinomas in other organs, thymoma, oral hairy leukoplakia) and a number of lymphoproliferative disorders. The latter are mostly B-cell proliferations such as infectious mononucleosis (acute, chronic or fatal type), Burkitt's lymphoma and lymphomas associated with immunodeficiency (eg, in AIDS and post-transplant patients). More recently, EBV has also been associated with some T-cell neoplasms and more consistently with Hodgkin's disease. Specialized molecular pathological techniques are required for the identification of EBV genes and gene products in tissue sections. We have used (1) in situ nucleic acid hybridization (ISH) with EBV-specific DNA probes to detect EBV genomes and (2) recently developed monoclonal antibodies against EBV latent (EBNA 1, EBNA 2, LMP), switch (ZEBRA) and replicative infection proteins (EA, VCA, MA) for the immunohistochemical demonstration of EBV gene expression in tissues from a variety of EBV-positive epithelial and lymphoproliferative lesions in both immunocompetent and immunodeficient patients. In particular, we have examined cases of AIDS-related lymphoma (ARL) and shown that (1) In 2 studies, EBV genomes could be detected in 43 and 50% of ARL using ISH (based on 63 and 24 cases, respectively); (2) The presence of EBV was related to the histological type of ARL. Immunoblast-rich ARL contained EBV in 73%, whereas the Burkitt's-type in this respect was similar to nonendemic Burkitt's tumors with 20-32% being EBV-positive; (3) EBV-encoded latent proteins with transforming capacity were expressd in 8 of 10 (80%; LMP) and 3 of 10 (30%; EBNA 2) of the EBV-positive ARL in which frozen tissue (n=13) was available for analysis; (4) Induction of the EBV replicative (productive) infection cycle could be demonstrated in tumor cells of some cases of ARL. The ZEBRA switch protein could be detected in 6 of 10 EBV-positive cases, EA in 4 of these, and VCA and MA in only one case. This indicates that while the EBV lytic cycle may be initiated in some ARL, production of infectious virions is incomplete in most cases.
Keywords: Antibodies, Monoclonal Antigens, Viral/GENETICS Burkitt's Lymphoma/*GENETICS DNA-Binding Proteins Gene Expression Genes, Switch Genes, Viral/GENETICS Genome, Viral Herpesvirus 4, Human/*GENETICS/PHYSIOLOGY Human Infectious Mononucleosis/*GENETICS Lymphoma, AIDS-Related/GENETICS Lymphoma, T-Cell/*GENETICS Nucleic Acid Hybridization Proteins/GENETICS Virus Replication/GENETICS ABSTRACT
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Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
