Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
CHARACTERIZATION OF RHESUS MACAQUE B LYMPHOBLASTOID CELL LINES INFECTED WITH SIMIAN TYPE D RETROVIRUS
Diss Abstr Int [B]; 52(5):2492 1991. Unique Identifier : AIDSLINE ICDB/92679523 Van Kuyk RW; Univ. of California, Davis
Abstract:
Simian AIDS (SAIDS) is a fatal immunosuppressive disease in rhesus macaques caused by a simian type D retrovirus designated SAIDS-associated retrovirus (SRV). This syndrome shows many clinical similarities to human AIDS. To investigate the cellular basis of immune dysfunction in SRV infection, we studied the interactions of SRV serotype 1 (SRV-1) with rhesus macaque B lymphoblastoid cell lines (B-LCL). In addition, uninfected and SRV-1-infected B-LCL were used to determine the presence of virus-specific cytotoxic cells in PBMC obtained from SRV-1-infected macaques. Procedures were optimized for establishing B-LCL by immortalization of macaque B lymphocytes with rhesus Epstein-Barr virus (EBV). Phenotypic characterization of the B-LCL showed expression of B-cell surface markers and secretion of immunoglobulins of the IgG or IgM isotypes. In addition, the B-LCL release EBV which transform rhesus macaque B cells. Cultures of B-LCL were productively infected in vitro with SRV-1. Several B cell lines infected with SRV-1 showed downregulation of MHC class II antigen expression, whereas levels of MHC class I antigen remained unchanged. Infection of B-LCL with SRV-1 did not change the level of secreted immunoglobulins. Rhesus EBV was also used to obtain B-LCL from macaques infected with SRV-1; these cell lines were found to release infectious SRV-1. Cytopathic effects were not observed in macaque peripheral blood mononuclear cells (PBMC) or B-LCL infected with SRV-1. However, SRV-1 had immunosuppressive properties because the virus inhibited mitogen-induced proliferation in macaque PBMC. In addition, infection of PBMC with SRV-1 reduced natural killer activity. To study cell-mediated immune responses in infected rhesus macaques, uninfected and SRV-1-infected B-LCL were used as targets for cytotoxic PBMC obtained from viremic and nonviremic monkeys. Chromium release assays showed that viremic monkeys had cytotoxic activity against SRV-1-infected B-LCL but not against uninfected B-LCL. Thus, investigations on the interactions of SRV-1 with B cells will be useful for elucidating mechanisms involved in the immunopathogenesis of primate retroviruses. In addition, SRV-1 infection of B cell lines is a useful system for analyzing antiviral immune responses in infected macaques. (Full text available from University Microfilms International, Ann Arbor, MI, as Order No. AAD91-25117).
Keywords: Animal Antigens, Surface/ANALYSIS B-Lymphocytes/IMMUNOLOGY/*MICROBIOLOGY Cell Line Immunoglobulins/ANALYSIS Killer Cells, Natural/IMMUNOLOGY Macaca mulatta Retroviruses Type D, Simian/*IMMUNOLOGY Simian Acquired Immunodeficiency Syndrome/*IMMUNOLOGY THESIS 920630
M9261018
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Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
