Color-monitored solid-phase multiple peptide synthesis under low-pressure continuous-flow conditions. NLM AIDSLINE Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

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Color-monitored solid-phase multiple peptide synthesis under low-pressure continuous-flow conditions.

Pept Res. 1990 Jul-Aug;3(4):182-93. Unique Identifier : AIDSLINE MED/92182584
Krchnak V; Vagner J; Research Institute for Feed Supplements and Veterinary Drugs,; Prague, Czechoslovakia.

Abstract: Exemplified on fifty peptides derived from proteins of the human immunodeficiency virus (HIV), we summarize our experiences with rapid solid-phase multiple peptide synthesis under low-pressure continuous-flow conditions on standard polystyrene-based resin in a series of concatenated flow reactors with adjustable volume. Alternatively, multiple batchwise synthesis in plastic syringes may be used as a still simpler but a more elaborate procedure. Completeness of acylation of resin-bound free amino groups was monitored by the acid-base indicator bromophenol blue. Efficiency of the coupling reaction was increased by exposure to elevated temperature in an ultrasonic bath. Up to 100 mg of crude peptides were purified on a mass-overloaded Vydac C18 reversed-phase semipreparative HPLC column in one run, this procedure ensuring high recovery of homogeneous peptides. The peptides synthesized were serologically relevant to the detection of anti-HIV-1 antibodies in positive sera.
Keywords: Acylation Amino Acid Sequence Antigens Chromatography, High Pressure Liquid Equipment and Supplies Molecular Sequence Data Peptides/*CHEMICAL SYNTHESIS/IMMUNOLOGY/ISOLATION & PURIF Pressure Syringes Ultrasonics JOURNAL ARTICLEKWDacylationaminoacidsequenceantigenschromatography,highpressureliquidequipmentandsuppliesmolecularsequencedatapeptides/KWDchemicalsynthesis/immunology/isolation&purifpressuresyringesultrasonicsjournalarticle
920630
M9260995

Copyright © 1992 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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