[Preparation of immunoenzyme conjugates of beta-lactamase from Bacillus licheniformis 749/c and horseradish peroxidase with human antibodies to HIV-1]

Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

[Preparation of immunoenzyme conjugates of beta-lactamase from Bacillus licheniformis 749/c and horseradish peroxidase with human antibodies to HIV-1]

Antibiot Khimioter. 1991 Nov;36(11):15-7. Unique Identifier : AIDSLINE MED/92171650
Sazykin AIu; Eremin SA; Egorov AM; Starov AI; Sakaian IA; Matsevich GR; Gibadulin RA

Abstract: By using three different linkage methods with carbodiimide, glutaraldehyde and periodite, immunoenzyme conjugates of beta-lactamase from Bacillus licheniformis 749/c and horse radish peroxidase with human antibodies to HIV-1 were prepared. The human antibodies were purified by the affinity procedure on Protein-A-Sepharose 6B. The conjugates were tested in a solid phase immunoenzymatic system for the HIV-1 antigen. It was shown that the conjugates prepared by the carbodiimide linkage method had the highest titer, the beta-lactamase conjugate being superior by its titer to the respective peroxidase conjugate. In the lyophilized state the conjugates prepared with the carbodiimide linkage method were stable.

Keywords: beta-Lactamases/*IMMUNOLOGY Bacillus/*ENZYMOLOGY English Abstract Horseradish Peroxidase/*IMMUNOLOGY Human HIV Antibodies/*IMMUNOLOGY Immunoenzyme Techniques JOURNAL ARTICLE
920630
M9260929

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