HUMAN B CELL LINES EXPRESS THE INTERFERON GAMMA GENE (MEETING ABSTRACT)

Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

HUMAN B CELL LINES EXPRESS THE INTERFERON GAMMA GENE (MEETING ABSTRACT)

Proc Annu Meet Am Assoc Cancer Res; 33:A1792 1992. Unique Identifier : AIDSLINE ICDB/92684317
Dayton M; Knobloch TJ; Benjamin D; CHRI 1232, Ohio State Univ., Columbus, OH 43210

Abstract: IFN gamma production has been attributed exclusively to activated T and NK cells. We sought to determine whether B cells express IFN gamma. We studied 28 B cell lines (BCL) including EBV+ normal lymphoblastoid BCL (n=7), EBV+ BCL derived from patients with Burkitt's lymphoma with (n=6) or without AIDS (n=8), as well as 7 EBV- BCL. All cell lines were studied by reverse transcription polymerase chain reaction. We found constitutive expression of IFN gamma in every BCL. The tumor promoters PMA and teleocidin appeared to enhance this IFN gamma expression. The 517-bp amplicons spanning the protein-coding region of the IFN gamma mRNA from representative lines of each group were sequenced, definitively establishing that B cell IFN is identical to IFN gamma from activated T cells and is not altered by derivation of the BCL from AIDS patients or by EBV status. Detection of IFN gamma in all of the BCL suggests that the IFN gamma gene is broadly expressed by human B cells. Our data imply that human B cells can be activated to produce IFN gamma, further enmeshing them in the dynamics of immunoregulation.

Keywords: Acquired Immunodeficiency Syndrome/IMMUNOLOGY B-Lymphocytes/*IMMUNOLOGY Burkitt's Lymphoma/IMMUNOLOGY Comparative Study *Gene Expression Human Interferon Type II/*GENETICS Lymphocyte Transformation Polymerase Chain Reaction RNA, Messenger/GENETICS T-Lymphocytes/IMMUNOLOGY Tumor Cells, Cultured ABSTRACT
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