CLONING AND SEQUENCING OF MMTV ENV GENE-LIKE SEQUENCES FROM HUMAN BREAST CANCER (MEETING ABSTRACT)

Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

CLONING AND SEQUENCING OF MMTV ENV GENE-LIKE SEQUENCES FROM HUMAN BREAST CANCER (MEETING ABSTRACT)

Proc Annu Meet Am Assoc Cancer Res; 33:A2337 1992. Unique Identifier : AIDSLINE ICDB/92684893
Wang Y; Pogo BG; Holland JF; Dept. of Neoplastic Diseases, Mount Sinai Sch. of Medicine, New; York, NY 10029

Abstract: Using polymerase chain reaction (PCR) we have found that sequences homologous to the unique 600-bp region of the env gene of mouse mammary tumor virus (MMTV) were present in 33% of the human breast tumors investigated, but not in normal tissues, nor in other human tumors analyzed. The frequency of detection was higher in samples from patients with a family history of breast carcinoma. Cloning of the PCR product was performed using the Invitrogen plasmid vector. Sequencing of the cloned sequences revealed that they were highly homologous to the MMTV env gene, but not to human endogenous retroviral sequences. Using the cloned sequences as probes for Southern blot hybridization, it was possible to detect them in DNA from breast carcinomas, but not in normal breast tissue nor in other tumor DNA. These sequences could be the result of recombination between endogenous viral-like sequences during tumorigenesis or of integrated exogenous viral sequences.

Keywords: Base Sequence Blotting, Southern Breast/PHYSIOLOGY Breast Neoplasms/*GENETICS/*MICROBIOLOGY Cloning, Molecular Comparative Study DNA/GENETICS/ISOLATION & PURIF DNA, Neoplasm/GENETICS/ISOLATION & PURIF Female *Genes, env Human Mammary Tumor Viruses, Mouse/*GENETICS Polymerase Chain Reaction/METHODS Reference Values Retroviridae/GENETICS Sequence Homology, Nucleic Acid ABSTRACT
920730
M9271103

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