GENOMIC VARIABILITY OF HTLV-I (MEETING ABSTRACT) NLM AIDSLINE Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

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GENOMIC VARIABILITY OF HTLV-I (MEETING ABSTRACT)

Proc Annu Meet Am Assoc Cancer Res; 33:A2394 1992. Unique Identifier : AIDSLINE ICDB/92684919
Pelloquin KF; Kagoshima SS; Pasteur Merieux, Marcy l'Etoile, France

Abstract: The genomic variability of HTLV-I was tested by PCR and direct sequencing in the ex vivo DNA from 4 Japanese patients (pts; 2 with TSP/HAM and 2 with ATL), 3 Caribbean pts (2 with TSP/HAM, 1 with ATL), and 2 Ivory Coast pts (1 with TSP/HAM, 1 with B lymphoma) involving parts of the LTR (nt 31-768) of the env gene (nt 5684-6151), and the px II, III and IV coding frames (nt 7201-8440). Genomic variations were observed mainly in the U3 sequence of the LTR and in the px IV coding frame. Further, 30 HTLV-I proviral samples (17 from Japan, 6 from Caribs, 5 from Africa, 1 from China and 1 from USA) were amplified in their LTR regions (nt 31-768) and digested by 6 enzymes having restriction sites modified by mutations, within the LTR region. The restriction polymorphism of the LTR observed in this series suggests the existence of 3 genomic subtypes. No specific mutation could be linked to either ATL or TSP/HAM.
Keywords: Base Sequence DNA, Viral/GENETICS/ISOLATION & PURIF Genes, env *Genes, Viral Human HTLV-I/*GENETICS/ISOLATION & PURIF HTLV-I Infections/*MICROBIOLOGY Mutation Polymerase Chain Reaction/METHODS Polymorphism (Genetics) Repetitive Sequences, Nucleic Acid Restriction Mapping *Variation (Genetics) ABSTRACTKWDbasesequencedna,viral/genetics/isolation&purifgenes,envKWDgenes,viralhumanhtlv-i/KWDgenetics/isolation&purifhtlv-iinfections/KWDmicrobiologymutationpolymerasechainreaction/methodspolymorphism(genetics)repetitivesequences,nucleicacidrestrictionmappingKWDvariation(genetics)abstract
920730
M9271102

Copyright © 1992 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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