Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
REGULATION OF VIRAL GENE EXPRESSION
Diss Abstr Int [B]; 52(1):79 1991. Unique Identifier : AIDSLINE ICDB/92677128 Williams JL; Univ. of California, Los Angeles
Abstract:
Chapter 1 provides knowledge concerning adenovirus and human T-cell leukemia viruses. Part 1 concerns isolation of adenovirus from different sources and describes recovery of virus in lymphoid cells. Part 2 describes functions of adenovirus E1a and E3 proteins and was written in order to relate known activities of E1a with cellular transformation and to describe the role of E3 during viral infection. This section serves as background information not directly related to most of my thesis and may not require as diligent reading as other sections. However, the latter portion concerning E3 function is relevant and should be read. The third section addresses what is known about transcription factors which bind E1a inducible promoters. The section concerning the E3 promoter should be read. Finally, the latter part of the Introduction concerns the HTLV tax and rex proteins and the transcription factor NF-kB and is relevant to my work. Information regarding expression from the E3 promoter is provided in Chapter 2. A high-affinity NF-kB motif is critical for activity in lymphoid cells but not in an epithelial cell line. Chapter 3 compares sequences in the E3 promoter required for HTLV tax and E1a inducibility and finds that like E1a, there appears to be no unique element in the E3 promoter needed for tax activation which can be separated from basal activity. Synergistic activation of E3 by tax and E1a suggests possible differences in function between these two proteins. The appendix describes a hybrid promoter which is responsive to E1a but not to tax. Chapter 4 describes a mutant in the HTLV II tax protein which destroys activation of the HTLV promoter but retains high levels of activation of the E3 promoter, suggesting that tax may interact with different cellular factors. Mutagenesis of the GM-CSF promoter and the effects upon tax inducibility are described in Chapter 5. Chapter 6 demonstrates that the HIV rev and the HTLV rex proteins are functionally equivalent in an assay utilizing the rev responsive element. The supplement to this section also demonstrates equivalence between the HTLV I and II rex proteins. (Full text available from University Microfilms International, Ann Arbor, MI, as Order No. AAD91-15456).
Keywords: Adenoviridae/*GENETICS *Gene Expression Regulation, Viral Gene Products, rex/PHYSIOLOGY Gene Products, tax/PHYSIOLOGY *Genes, Viral HTLV-BLV Viruses/*GENETICS Oncogene Proteins, Viral/PHYSIOLOGY Promoter Regions (Genetics) THESIS
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Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
