[Novel neutral glycolipid associated with adult T-cell leukemia and its pathological biochemistry] NLM AIDSLINE Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

Click here to return to AIDSLINE main menu
DonateNow
Print this Article


[Novel neutral glycolipid associated with adult T-cell leukemia and its pathological biochemistry]

Hum Cell. 1989 Mar;2(1):56-62. Unique Identifier : AIDSLINE MED/92031240
Ono K; Yamamoto K; Okumura K; Laboratory for Cell Biology, Pharma Research Laboratories,; Hoechst Japan, Ltd., Kawagoe.

Abstract: During attempts to make antibodies cross-reactive with human lymphocytes, we established a monoclonal antibody (VJ-41) from the alloimmunization of mice, that is, B10. A (3R) anti-B10. A (5R). VJ-41 reacted with all cases of freshly isolated adult T-cell leukemia cells (36 cases) but not with cells from other hematological disorders (more than 50 cases). Human T-cell leukemia virus type I healthy carriers also seemed to possess these VJ-41 antigen positive cells. However, in vitro established adult T-cell leukemia cell lines did not show the reactivity with VJ-41. Normal lymphocytes from humans or mice apparently did not carry this antigen, but mitogen activated lymphocytes or some in vitro maintained malignant cell lines of both human and mouse origins showed positive reaction. Having established solid phase radioimmunoassay to detect the VJ-41 antigen in plasma, it was found that healthy human T-cell leukemia virus type I carriers, but not the majority of adult T-cell leukemia patients, predominantly possessed this antigen. Even though immunochemical characterizations of cellular materials were unsuccessful, a certain neutral glycolipid was detected from healthy human T-cell leukemia virus type I carrier plasma by using thin-layer chromatography and immunostaining with the VJ-41 antibody.
Keywords: Animal Antibodies, Monoclonal/IMMUNOLOGY Antigens, Viral/*ANALYSIS/IMMUNOLOGY Carrier State Chromatography, Thin Layer English Abstract Glycolipids/*ANALYSIS/IMMUNOLOGY Human HTLV-I/IMMUNOLOGY Leukemia, T-Cell/IMMUNOLOGY/*METABOLISM Mice Radioimmunoassay JOURNAL ARTICLEKWDanimalantibodies,monoclonal/immunologyantigens,viral/KWDanalysis/immunologycarrierstatechromatography,thinlayerenglishabstractglycolipids/KWDanalysis/immunologyhumanhtlv-i/immunologyleukemia,t-cell/immunology/KWDmetabolismmiceradioimmunoassayjournalarticle
920228
M9220872

Copyright © 1992 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1992. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1992. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .