Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
A monoclonal antibody which blocks infection with feline immunodeficiency virus.
Int Conf AIDS. 1992 Jul 19-24;8(1):Mo13 (abstract no. MoA 0042). Unique Identifier : AIDSLINE ICA8/92399999 Hosie MJ; Willett BJ; Dunsford TH; Neil JC; Jarrett O
Abstract:
OBJECTIVES: Although cells expressing the feline homologue of CD4 (fCD4) are highly susceptible to infection with feline immunodeficiency virus (FIV), it has been shown that FIV infects fCD4- cells, such as feline macrophages, astrocytes and fCD8+ T-cells. Similarly, HIV has been shown to infect various CD4- cell lines, such as glioma, neural cells, muscle cells, fibroblasts and trophoblasts, implying that CD4 may not be the only HIV receptor. We therefore screened cell-surface-reactive monoclonal antibodies other than those directed against fCD4 for their capacity to block FIV infection. METHODS: Blocking experiments were carried out on two cell lines; a fCD4+ T-cell line named Q201 and a feline fCD4- fibroblast line CRFK. Cells were incubated with one of three isotype-matched antibodies: anti-fCD4, anti-feline IgA and a murine monoclonal antibody designated vpg15. After incubation with antibody, FIV was added to the cultures. Following a one hour incubation the cells were washed, maintained in culture and monitored for evidence of FIV infection by detection of FIV p24. RESULTS: After infection of Q201 cells with FIV, the culture fluid from cells which had been treated with no antibody, with anti-fCD4 or with anti-feline IgA contained detectable p24 by day 4, the level rising sharply to day 7. In contrast, pretreatment with vpg15 blocked infection, no p24 being detected in the culture supernatant during the experiment. When CRFK cells were incubated with two-fold dilutions of vpg15 prior to FIV infection, a dose-dependent blocking of infection was seen. CONCLUSIONS: Since vpg15 blocked FIV infection the cell surface molecule which it recognises has an important role in infection and may represent a non-fCD4 host cell surface receptor for FIV.
Keywords: Animal Antibodies, Monoclonal/*IMMUNOLOGY Antigens, CD4/*IMMUNOLOGY Cats Cells, Cultured Comparative Study CD4-Positive T-Lymphocytes/*MICROBIOLOGY Fibroblasts/MICROBIOLOGY Immunodeficiency Virus, Feline/*IMMUNOLOGY/PHYSIOLOGY Receptors, HIV/*IMMUNOLOGY Virus Replication ABSTRACT 921230
M92C5348
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Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
