Small amino acid change in the HIV-1 V3 loop can affect viral infectivity and not cytopathicity. NLM AIDSLINE Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

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Small amino acid change in the HIV-1 V3 loop can affect viral infectivity and not cytopathicity.

Int Conf AIDS. 1992 Jul 19-24;8(1):Mo20 (abstract no. MoA 0072). Unique Identifier : AIDSLINE ICA8/92400032
Cheng-Mayer C; Cancer Research Institute, Univ. of California, San Francisco.

Abstract: OBJECTIVE: To examine whether single amino acid change in env, specifically a region of gp120 that encompasses the V3 loop affects infectivity and cytopathicity of HIV-1 in vitro. METHODS: Biologic properties of site-directed mutants of HIV-1 were determined. Sequence analyses confirmed the nature of the mutations and two independent clones of each mutant were studied with similar results. RESULTS: TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSIONS: These studies of SF13 and SF2 mutants showed (1) amino acid (aa) change in the V3 loop had no effect on CPE (e.g., SF13mu1,SF2mu1) (2) complementarity of amino acids at positions 311 and 319 within the V3 loop (e.g., histidine/tyrosine in WT vs histidine/histidine in SF13mu3, SF2mu3) influences HIV-1 infectivity (3) a single aa change in the conserved DIRQAHC sequence of the V3 loop abolishes infectivity (e.g., SF2mu1 vs SF2mu5) (4) a single aa change outside the V3 loop has been found to affect viral infectivity. All these results indicate that the structure of the V3 loop, influenced by amino acid changes within and outside of the loop region, governs HIV-1 infectivity and cytopathicity.
Keywords: Amino Acid Sequence Cell Fusion/*GENETICS Cells, Cultured Comparative Study Cytopathogenic Effect, Viral/*GENETICS *Genes, env Human HIV Envelope Protein gp120/*GENETICS/PHYSIOLOGY HIV-1/GENETICS/*PHYSIOLOGY Molecular Sequence Data Mutagenesis, Site-Directed Peptide Fragments/*GENETICS/PHYSIOLOGY Virus Replication/*GENETICS ABSTRACTKWDaminoacidsequencecellfusion/KWDgeneticscells,culturedcomparativestudycytopathogeniceffect,viral/KWDgeneticsKWDgenes,envhumanhivenvelopeproteingp120/KWDgenetics/physiologyhiv-1/genetics/KWDphysiologymolecularsequencedatamutagenesis,site-directedpeptidefragments/KWDgenetics/physiologyvirusreplication/KWDgeneticsabstract
921230
M92C5315

Copyright © 1992 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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