Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
High expression of c-myc gene is associated with chromosomal translocation in AIDS-related B-cell lymphoma.
Int Conf AIDS. 1992 Jul 19-24;8(2):A16 (abstract no. PoA 2078). Unique Identifier : AIDSLINE ICA8/92400371 Rasheed S; Kaplan B; Shieh CK; Li Z; Laboratory of Viral Oncology and AIDS Research, USC School of; Medicine, Los Angeles 90032-3626.
Abstract:
OBJECTIVE: To understand the molecular mechanism/s of B-cell lymphoma development in patients with AIDS. METHODS: A lymphoid cell line was established in vitro from the circulating tumor cells present in the peripheral blood of a patient with AIDS-related lymphoma. Cells were characterized by immunophenotyping, cytogenetic and molecular analyses including Southern and Northern blot hybridization, and PCR using specific probes for the detection of viral and cellular genes. RESULTS: By immunophenotyping, the cell line expresses only B-cell surface markers and no T-cell or monocyte/macrophage markers were detected. Using HIV-specific gag-gene primer pairs (sk-38/39) and PCR, an integrated HIV genome was not detected in the cellular DNA. However, Epstein Barr virus DNA was detected by the Southern blot hybridization of cellular DNA. Cytogenetic analysis of cells indicated chromosomal translocation of 8:14 with a trisomy of chromosome #12. The cells expressed significantly high levels of c-myc gene RNA and the immunoglobulin heavy chain (IgH) gene was rearranged. However, the T-cell receptor alpha-chain gene in the 3' region of the rearranged c-myc was not detected. CONCLUSIONS: We conclude that the cell line established in vitro from an AIDS patient with B-cell lymphoma consists exclusively of monoclonal tumor cells of B-cell phenotype. The 8:14 translocation and the IgH chain rearrangements are consistent with the activation of c-myc gene expression. However, the 3' region of the c-myc gene translocation and the elevated levels of c-myc RNA do not involve the T-cell receptor alpha-chain as reported for some non-HIV associated B-cell lymphoma. Our data also indicate that lymphomagenesis does not require HIV integration in the B-cell DNA, but is a result of complex interactions between viral and cellular factors produced in HIV-infected individuals. A discussion of some novel factors will be presented.
Keywords: Acquired Immunodeficiency Syndrome/*COMPLICATIONS/MICROBIOLOGY *Chromosomes, Human, Pair 12 Chromosomes, Human, Pair 14/*ULTRASTRUCTURE Chromosomes, Human, Pair 8/*ULTRASTRUCTURE DNA, Neoplasm/ANALYSIS Gene Expression Regulation, Neoplastic Gene Rearrangement, B-Lymphocyte, Heavy Chain *Genes, myc Herpesvirus 4, Human/ISOLATION & PURIF Human HIV/ISOLATION & PURIF Lymphoma, B-Cell/ETIOLOGY/*GENETICS/METABOLISM/MICROBIOLOGY *Translocation (Genetics) *Trisomy Tumor Cells, Cultured/METABOLISM/MICROBIOLOGY/ULTRASTRUCTURE Tumor Stem Cells/METABOLISM/MICROBIOLOGY/ULTRASTRUCTURE ABSTRACT 921230
M92C4976
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Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
