Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
MORPHOLOGICAL AND PHENOTYPICAL CHANGES IN EBV-POSITIVE LYMPHOBLASTOID CELLS INFECTED BY HIV-1 (MEETING ABSTRACT)
Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 14, 1991.. Unique Identifier : AIDSLINE ICDB/92682370 De Rossi A; Calabro ML; D'Andrea E; Panozzo M; Saggioro D; Mammano F; Gallo P; Roncella S; Ferrarini M; Chieco-Bianchi L; Inst. of Oncology, Univ. of Padova, Padova, Italy
Abstract:
To study the mode of HIV-1 infection in EBV-positive lymphoblastoid cell lines (LCLs), as well as the molecular and biological features of EBV- and HIV-1-infected B cells, 3 spontaneous LCLs from HIV-1 seropositive individuals and 2 LCLs derived in vitro by infection with supernatant from the B95.8 marmoset cell line, were superinfected with HIV-1, strain IIIB. HIV-1 expression was monitored by reverse transcriptase and p24 antigen capture assays. HIV-1 infectivity was evaluated by CAT assay on H938 cells (containing the HIV-1 LTR upstream of the CAT gene) infected with filtered LCL supernatants. IL-6, TNF-alpha, and TNF-beta cytokine expressions were studied by Northern blot analysis, and their levels in LCL supernatants were assessed by commercial kits. All LCLs were successfully infected by HIV-1 as measured by reverse transcriptase and p24 levels in the supernatants. Both HIV-1-infected cells and their supernatants efficiently transactivated the CAT gene when employed in cocultivation or infection experiments with H938 cells. No cytopathic effect was observed in the LCLs; nevertheless, the HIV-1 released by B cells conserved its ability to form syncytia and lyse CD4+ T lymphocytes. Following HIV-1 infection, B cells showed a decrease in CD23 and CD39 antigens and an increase in CD10 marker expression. Not all cells expressed viral antigens, but all CD10+ cells also coexpressed the gp 120 HIV-1 protein. Moreover, HIV-1 infected LCLs were able to form more agar colonies than control LCLs. Preliminary results also indicate a down expression of IL-6 and TNF-alpha cytokines in the HIV-1 infected B cells. The morphological and phenotypical changes which occurred in the infected cells might be relevant to the pathogenesis of non-Hodgkin's B lymphomas, which occur frequently in AIDS patients.
Keywords: Antigens, CD/ANALYSIS Antigens, Differentiation/ANALYSIS Antigens, Differentiation, B-Lymphocyte/ANALYSIS Antigens, Neoplasm/ANALYSIS B-Lymphocytes/IMMUNOLOGY Burkitt's Lymphoma/ETIOLOGY/GENETICS/*IMMUNOLOGY Chloramphenicol Acetyltransferase/GENETICS Cytopathogenic Effect, Viral/GENETICS CD4-Positive T-Lymphocytes *Herpesvirus 4, Human Human HIV Core Protein p24/GENETICS HIV Envelope Protein gp120/ANALYSIS HIV Infections/COMPLICATIONS/*IMMUNOLOGY HIV-1/*IMMUNOLOGY Immunophenotyping Interleukin-6/GENETICS Lymphotoxin/GENETICS Receptors, Fc/ANALYSIS Trans-Activation (Genetics)/GENETICS Tumor Cells, Cultured Tumor Necrosis Factor/GENETICS ABSTRACT 920830
M9281110
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Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
