Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
LYMPHOMA INDUCTION BY HUMAN B CELLS IN SCID MICE (MEETING ABSTRACT)
Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 2, 1991.. Unique Identifier : AIDSLINE ICDB/92682361 Amadori A; Veronese ML; Mazza MR; Zamarchi R; D'Andrea E; Menin C; Del Mistro A; Leszl A; Panozzo M; Chieco-Bianchi L; Inst. of Oncology, Univ. of Padova, Padova, Italy
Abstract:
The severe combined immunodeficiency (SCID) mouse represents a new interesting model for both immunologic and oncologic investigations. Several workers observed the high frequency with which SCID mice, injected ip with peripheral blood mononuclear cells (PBMC) from Epstein-Barr virus (EBV)-positive (+) donors, develop B-cell lymphomas; it is likely that latently EBV-infected B-cell precursors undergo expansion in SCID mice in the absence of immunologic control. To better understand the mechanisms underlying this phenomenon, we studied the effect of different PBMC subpopulations. When SCID mice were injected with 50-100 x 10(6) unfractionated PBMC from EBV+ donors, B-cell lymphomas were observed in virtually all injected animals. These lymphomas showed phenotypic and genotypic characteristics comparable to those described by other workers: they were multiple in origin, and showed a phenotype compatible with a B-cell origin (CD19+, DR+, CD39+, LFA-1+, LFA-3+). All samples were also EBV+; Southern blot analysis of Ig gene rearrangement showed oligo/monoclonal patterns in the different districts. Monocyte depletion of PBMC by plastic adherence did not apparently change the frequency of lymphoma development; on the other hand, when PBMC were depleted of T cells, no lymphoma was observed, despite the fact that a 5- to 10-fold enrichment in B cells was achieved in the injected populations, compared to unfractionated PBMC. Moreover, when PBMC were selectively deprived of CD4+ or CD8+ cells by monoclonal antibody treatment, followed by immunomagnetic separation with antimouse Ig-coated magnetic beads, lymphomas were observed in SCID mice injected with both cell populations. Thus, either CD4+ or CD8+ cells seemed to be strictly required for sustaining EBV+ B-cell proliferation in SCID mice.
Keywords: Animal Antigens, CD/ANALYSIS Antigens, Differentiation, B-Lymphocyte/ANALYSIS Antigens, Surface/ANALYSIS CD4-Positive T-Lymphocytes Gene Rearrangement Genes, Immunoglobulin/GENETICS Herpesvirus 4, Human Human HLA-DR Antigens/ANALYSIS Immunophenotyping Leukocyte Count Leukocytes, Mononuclear/IMMUNOLOGY Lymphocyte Function-Associated Antigen-1/ANALYSIS Lymphoma, B-Cell/*IMMUNOLOGY Membrane Glycoproteins/ANALYSIS Mice, SCID T-Lymphocytes T-Lymphocytes, Suppressor-Effector ABSTRACT
920830
M9281086
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Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
