Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
CHARACTERIZATION OF EBV-POSITIVE LYMPHOBLASTOID B CELL LINES ORIGINATED FROM HIV-SEROPOSITIVE PATIENTS WITH AND WITHOUT LYMPHOMAS (MEETING ABSTRACT)
Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Disease. October 6-11, 1991, Padova/Venice, Italy, p. 78, 1991.. Unique Identifier : AIDSLINE ICDB/92682419 Roncella S; Francia Di Celle P; Cutrona G; Foa R; Carbone A; Rowe M; Ferrarini M; Istituto Nazionale per la Ricerca sul Cancro, Laboratorio di; Immunologia Clinica, Genova, Italy
Abstract:
EBV-positive B-cell lines (LCLs) were originated spontaneously,in vitro, from HIV-seropositive patients (pts). Particular caution was taken to ensure that the LCLs were originated by an in vitro expansion of in vivo latently infected B cells rather than by B cells infected by the EBV released in vitro. The LCLs from HIV-seropositive pts were comprised of normal lymphoblastoid cells. The cells had the surface differentiation markers CD23, CD38, CD39 and did not express the CD10 antigen which is found on malignant cells; they failed to form colonies in agar or to grow in nude mice and displayed normal karyotypes. Moreover, these cells expressed abundant LFA-1, LFA-3 and ICAM-1 surface molecules and grew in culture in tight clumps. These cells, therefore, were likely to represent the expansion of normal B cells infected by EBV that were found to be accumulated in the peripheral blood and in the lymphoid organs. LCLs obtained from AIDS pts with non-HD lymphomas were comprised of a mixture of normal and malignant lymphoid cells as could be assessed by cloning experiments. The malignant cells expressed CD10 but not other differentiation markers and had low quantities of surface adhesion molecules. They had a rearranged c-myc and other chromosomal abnormalities. Some of the malignant cells were EBV-negative, other EBV-positive with a poor expression of EBV-latent antigens. In one case with EBV-positive malignant cells, evidence was obtained indicating that EBV-infection occurred following c-myc rearrangement.
Keywords: Antigens, CD/METABOLISM Antigens, Differentiation/METABOLISM Antigens, Differentiation, B-Lymphocyte/METABOLISM Antigens, Neoplasm/METABOLISM Antigens, Surface/METABOLISM Cell Adhesion Molecules/METABOLISM Gene Rearrangement Herpesvirus 4, Human Human HIV Seropositivity/PATHOLOGY Lymphocyte Function-Associated Antigen-1/METABOLISM Lymphoma, B-Cell/GENETICS/METABOLISM/*PATHOLOGY Membrane Glycoproteins/METABOLISM Proto-Oncogene Proteins c-myc/GENETICS Receptors, Fc/METABOLISM Tumor Cells, Cultured ABSTRACT
920830
M9281084
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Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.
