Detection and isolation of Mycoplasma fermentans from urine of patients with AIDS. NLM AIDSLINE Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

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Detection and isolation of Mycoplasma fermentans from urine of patients with AIDS.

Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:134 (abstract no. G-4). Unique Identifier : AIDSLINE ASM91/0020292
Dawson MS; Wang R; Hayes M; Armstrong D; Budzko D; Lo SC; Kundsin R; ARP, Armed Forces Institute of Pathology, Washington, D.C.

Abstract: Mycoplasmas of the M. fermentans species, a rarely identified urogenital mycoplasma was shown to infect the kidneys of HIV positive patients with AIDS-associated nephropathy. We set out in this study to 1) detect M. fermentans in the urine sediments of HIV positive patients utilizing the polymerase chain reaction (PCR) and 2) to isolate M. fermentans from these urines. Using the synthetic oligonucleotide primer pairs, RW004 and RW005 which contain specific sequences within the insertion sequence-like genetic element found only in mycoplasmas of the M. fermentans species, 10 of 43 urine sediments obtained from 40 HIV positive patients at different stages of AIDS disease tested positive. M. fermentans was isolated and grown in mycoplasma cultures from three urine sediments obtained from two HIV positive individuals. Fifty urine sediments from age-matched HIV negative healthy controls tested negative for M. fermentans by PCR. M. fermentans was not isolated from any of the control urine samples. Our results show a high prevalence of the AIDS-associated mycoplasma, M. fermentans, in the urine from HIV positive patients but not from HIV negative non-AIDS controls.
Keywords: Acquired Immunodeficiency Syndrome/COMPLICATIONS/*MICROBIOLOGY Bacteriuria/COMPLICATIONS Genes, Bacterial Human Mycoplasma fermentans/GENETICS/*ISOLATION & PURIF Oligonucleotide Probes Polymerase Chain Reaction ABSTRACTKWDacquiredimmunodeficiencysyndrome/complications/KWDmicrobiologybacteriuria/complicationsgenes,bacterialhumanmycoplasmafermentans/genetics/KWDisolation&purifoligonucleotideprobespolymerasechainreactionabstract
920830
M9281081

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