Detection of HIV-1 transcropts and HIV-1 viremia by reverse-transcription polymerase chain reaction. NLM AIDSLINE Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

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Detection of HIV-1 transcropts and HIV-1 viremia by reverse-transcription polymerase chain reaction.

Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:334 (abstract no. T-4). Unique Identifier : AIDSLINE ASM91/0170292
Bagnarelli P; Clementi M; Menzo S; Pauri P; Valenza A; Varaldo PE; Manzin A; Institutes of Microbiology, University of Ancona and Trieste -; Italy.

Abstract: Aim of the research has been to define a molecular boundary between virus latency and active replication phase in course of HIV-1 infection. For this purpose PBLs and plasma from seropositive subjects were checked for the presence of HIV-1 specific transcipts and genomic RNA (HIV-1 viremia), respectively, using reverse transcription-polymerase chain reaction technique to amplify highly conserved sequences of gag and env ORFs. A high proportion (21/33; 64%) of seropositive subjects with more than 400/microliter CD4+ lymphocytes, as well as 25/28 (89%) immunodeficient patients (CD4+ lower than 400/microliter) show specific viral transcripts. The high majority (28/30; 93%) of subjects investigated for the presence of genomic HIV-1 RNA is viremic. These results indicate that: 1) detection of HIV-1 transcripts in PBLs of seropositive subjects during clinical latency allows the very early identification of patients requiring specific antiviral therapy; 2) a basal HIV-1 replication level is detectable as viremia in most infected subjects.
Keywords: Blood/*MICROBIOLOGY CD4-Positive T-Lymphocytes Genes, env Genes, gag Genes, Viral Human HIV Infections/MICROBIOLOGY HIV-1/*GENETICS/ISOLATION & PURIF/PHYSIOLOGY Leukocyte Count Polymerase Chain Reaction *Transcription, Genetic Virus Replication ABSTRACTKWDblood/KWDmicrobiologycd4-positivet-lymphocytesgenes,envgenes,gaggenes,viralhumanhivinfections/microbiologyhiv-1/KWDgenetics/isolation&purif/physiologyleukocytecountpolymerasechainreactionKWDtranscription,geneticvirusreplicationabstract
920830
M9281066

Copyright © 1992 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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