Detection of HIV-1 and -2 infection by the polymerase chain reaction (PCR).

Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

Detection of HIV-1 and -2 infection by the polymerase chain reaction (PCR).

Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:335 (abstract no. T-7). Unique Identifier : AIDSLINE ASM91/0200292
Brown V; Dela Torre N; Moore J; Fridlund C; George J; Schochetman g; Brattegaard K; Boateng E; Decock K; Ou CY; Centers for Disease Control. Atlanta, GA.

Abstract: PCR is an effective diagnostic tool for the identification of HIV-1 infection from clinical specimens. Due to the presence of cross-seroreactivity and mixed infections in regions of West Africa, the use of PCR to differentiate HIV-1 and -2 infections is important. However, difficulty is encountered in designing HIV-2 specific primers because HIV-2 exhibits a higher degree of genetic variation than HIV-1. Two HIV-2 isolates derived from the Cote d'Ivoire (CI) and the Cape Verde Islands were used for amplification. Lymphocytes from patients residing in CI were tested with primers derived from gag and env of HIV-1 and from the LTR, gag, pol, env and X of HIV-2. Amplified products were detected by hybridization protection assays with chemiluminescent probes or by Southern blotting with 32P-labeled oligonucleotide probes. Most of the HIV-2-specific primer pairs detected many but not all specimens from patients serologically positive for HIV-2. To maximize detection of HIV-1 and -2 infection, the use of multiple primer pairs is necessary.

Keywords: Blotting, Southern Genes, env Genes, gag Genes, pol Genes, Viral Human HIV Infections/*DIAGNOSIS/MICROBIOLOGY HIV Long Terminal Repeat HIV-1/GENETICS/*ISOLATION & PURIF HIV-2/GENETICS/*ISOLATION & PURIF Oligonucleotide Probes Polymerase Chain Reaction ABSTRACT
920830
M9281063

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1992. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1992. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .