Amplification of HIV gag and env genes from kidney biopsy archival material. NLM AIDSLINE Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

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Amplification of HIV gag and env genes from kidney biopsy archival material.

Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:335 (abstract no. T-8). Unique Identifier : AIDSLINE ASM91/0210292
Ferreira-Centeno A; Kimmel PL; Rodriguez ER; Farkas-Szallasi T; Abraham AA; Garrett CT; Departments of Pathology and Medicine, George Washington; University Medical Center, Washington D.C.

Abstract: Patients infected with the HIV virus may develop a form of renal disease known as HIV-associated nephropathy. To elucidate whether HIV could cause renal disease directly, we first examined renal tissue from 8 patients with kidney disease to determine if HIV genome was present. DNA from paraffin sections of renal biopsy was extracted by a modified Higuchi method. A sensitive nested polymerase chain reaction amplified target sequences within the gag and env regions of the HIV proviral genome. Target sequences were detected with 32P end labeled probes. Six of the patients were HIV positive. Four had focal glomerulosclerosis (FSGS), one IgA nephropathy and one had minimal change disease. The two control patients had FSGS but were HIV negative. gag and env target sequences were detected in all six HIV positive patients, but not from the two control patients. HIV genome was found in HIV infected patients with HIV nephropathy. The pathogenic role of viral infection is unknown. Currently, efforts to examine microdissected individual cells for the presence of HIV are in progress.
Keywords: Biopsy Gene Amplification *Genes, env *Genes, gag Genes, Viral Glomerulonephritis, IGA/COMPLICATIONS Glomerulosclerosis, Focal/COMPLICATIONS Human HIV/*GENETICS HIV Infections/COMPLICATIONS Kidney/*PATHOLOGY Kidney Diseases/COMPLICATIONS Polymerase Chain Reaction ABSTRACTKWDbiopsygeneamplificationKWDgenes,envKWDgenes,gaggenes,viralglomerulonephritis,iga/complicationsglomerulosclerosis,focal/complicationshumanhiv/KWDgeneticshivinfections/complicationskidney/KWDpathologykidneydiseases/complicationspolymerasechainreactionabstract
920830
M9281062

Copyright © 1992 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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