PCR analysis of HIV protease sequences from lab isolates. NLM AIDSLINE Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

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PCR analysis of HIV protease sequences from lab isolates.

Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:335 (abstract no. T-9). Unique Identifier : AIDSLINE ASM91/0220292
Fontenot G; Cohen C; Robinson J; Johnston K; Luftig R; Dept. of Microbiology, Immunology and Parasitology; LSU Medical; Center, New Orleans, LA.

Abstract: HIV replication involves a limited proteolysis of viral precursor proteins containing gag and gag-pol sequences by a specific viral protease. In order to characterize the HIV protease, sequences from: a) 5 patient isolates in New Orleans, b) 4 established strains grown in our lab, c) 10 strains in the Los Alamos database, and d) a new Ugandan isolate, were compared to determine sequence variability and show regions of high conservation. HIV proviral DNA was extracted from infected lymphocytes grown in tissue culture and amplified by PCR using specific primers flanking the protease region. Amplified DNA was sequenced using a modified technique of Sequenase Version 2.0(USB). Sequence data from the protease gene showed a 20% variation among different HIV strains at the amino acid level, including 7% non-conservative changes and 13% conservative changes. Additionally, four noncontiguous regions of the protease exhibited no amino acid changes between HIV strains studied. These areas included: (1) amino acids 1-9 (amino terminus); (2) 21-32 (protease active site); (3) 47-59 (flap region); and 76-88 (near carboxy terminus). These results are consistent with those obtained from structural and mutational studies done on HIV protease by other investigators. These findings may provide a basis for future HIV drug strategies.
Keywords: Amino Acid Sequence DNA, Viral/GENETICS Fusion Proteins, gag-pol/GENETICS Genes, gag HIV/PHYSIOLOGY HIV Protease/*GENETICS Polymerase Chain Reaction Proviruses/GENETICS Software Virus Replication ABSTRACTKWDaminoacidsequencedna,viral/geneticsfusionproteins,gag-pol/geneticsgenes,gaghiv/physiologyhivprotease/KWDgeneticspolymerasechainreactionproviruses/geneticssoftwarevirusreplicationabstract
920830
M9281061

Copyright © 1992 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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