Development of an automated enzyme immunoassay for the detection and quantitation of human immunodeficiency virus (HIV) antigen.

Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

Development of an automated enzyme immunoassay for the detection and quantitation of human immunodeficiency virus (HIV) antigen.

Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:335 (abstract no. T-11). Unique Identifier : AIDSLINE ASM91/0240292
Bauer R; Cole C; Baxter Diagnostics, Mundelein, IL.

Abstract: HIV is the etiologic agent of AIDS, and the demonstration of HIV antigen in patient serum or plasma samples correlates with disease progression. Further, monitoring HIV antigen levels during therapeutic intervention correlates with the therapeutic's efficacy. Thus, a method for the rapid and accurate quantitation of HIV antigen in serum or plasma will be useful as both diagnostic and prognostic indicators of HIV infection. An enzyme immunoassay (EIA) to detect and quantitate Human Immunodeficiency Virus (HIV) in plasma or serum has been developed for use on an automated instrument. The EIA employs paramagnetic microparticles and an enzyme conjugate substrate system. Anti-HIV immunoglobulins, which are attached to the microparticles, are incubated with sample. Following a programmed wash sequence, the conjugated is added and fluorogenic product is quantitated by kinetic measurements.

Keywords: Enzyme-Linked Immunosorbent Assay Human HIV Antigens/*ANALYSIS HIV Infections/DIAGNOSIS ABSTRACT
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