Comparison of whole cell viral lysate (VL), synthetic peptide (SP), and recombinant protein (RCP) EIA's for detection of HIV-1 antibody. NLM AIDSLINE Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

Click here to return to AIDSLINE main menu
DonateNow
Print this Article


Comparison of whole cell viral lysate (VL), synthetic peptide (SP), and recombinant protein (RCP) EIA's for detection of HIV-1 antibody.

Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:336 (abstract no. T-12). Unique Identifier : AIDSLINE ASM91/0250292
Johnson JE; Univ. Kentucky and VA Medical Center, Lexington, KY.

Abstract: Five hundred seventy five serum specimens received for HIV-1 antibody determination were tested prospectively by EIA using a whole cell viral lysate (Genetic Systems Corp.) a synthetic peptide (United Biomedical, Inc.) and a recombinant protein (Syva Co.). Concordance of all 3 antigens was noted for 558 of 575 (97%) specimens tested. Ninety (15.7%) specimens were positive and 468 (84.3%) were negative. Of the 16 discrepant results, 8 were non-specifically positive by VL and 7 were non-specifically positive by SP. The RCP ntigen gave no non-specific positive reactions, however there was one false-negative result. Retrospective testing by SP and RCP of 86 specimens indeterminant for HIV-1 antibody by VL, Western blot, and immunofluorescence also was performed. All 3 EIA screening methods agreed for 29 (33.7%) specimens. Forty five specimens were reproducibly reactive (rr) only by VL, 9 were rr by VL and SP, 2 were rr only by SP, and 1 was rr by VL and RCP. None of the 86 specimens were rr only by RCP. The results of both phases of this study indicate the specificity of the 3 antigens is RCP SP VL. As noted from the prospective phase, the sensitivity of the antigens appears to be equivalent.
Keywords: Blotting, Western Comparative Study Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique Human HIV Antibodies/*ANALYSIS HIV-1/*IMMUNOLOGY *Peptides Predictive Value of Tests Prospective Studies *Recombinant Proteins Reproducibility of Results ABSTRACTKWDblotting,westerncomparativestudyenzyme-linkedimmunosorbentassayfluorescentantibodytechniquehumanhivantibodies/KWDanalysishiv-1/KWDimmunologyKWDpeptidespredictivevalueoftestsprospectivestudiesKWDrecombinantproteinsreproducibilityofresultsabstract
920830
M9281058

Copyright © 1992 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1992. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1992. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .