1992

A:World united against AIDS. [videorecording].: Geneva, Switzerland : World Health Organization, [1988] 1 videocassette (21 min.) : sd., col. with b&w ; 1/2 in Unique Identifier : AIDSLINE AV/9200958A
Finding our way together. [videorecording]: [United States] : Rain Country Video, c1989 1 videocassette (28 min.) : sd., col. ; 1/2 in. + 1 leaflet Unique Identifier : AIDSLINE AV/9200941A; (Producer) People with AIDS and their caregivers discuss the special needs they have in dealing with AIDS. Two essential points are underscored: that caregivers also need caregivers, so that they can be strong, and that health should always be emphasized over sickness.
A:Right to care : help and health services for people with HIV/AIDS. [videorecording]: [Albany, N.Y.?] : The Department, c1990 1 videocassette (21 min.) : sd., col. ; 1/2 in Unique Identifier : AIDSLINE AV/9200907A; (Producer) This video explores the legal rights to health care in New York State for people with HIV or AIDS. Brief vignettes illustrate and discuss possible forms of discrimination against people with HIV/AIDS.
Gastrointestinal manifestations of AIDS : recorded at DDW 1990, San Antonio. [sound recording]: [Thorofare, N.J.] : American Gastroenterological Association, c1990 2 sound cassettes (ca. 105 min.) : analog (AGA clinical symposium) Unique Identifier : AIDSLINE AV/9200942A
Communicable disease control. [videorecording]: Naples, Fla. : ASVP, c1991 1 videocassette (ca. 28 min.) : sd., col. ; 1/2 in (Hands on skills series) Unique Identifier : AIDSLINE AV/9200866A; West KH; (Producer) Essential for all pre-hospital care providers, this program provides important information on communicable diseases such as Hepatitis, Hepatitis Non-A and Non-B, HIV, and childhood diseases that can spread from patient to rescuer, from care provider to care provider, or from your last patient to your next
AIDS, every day precautions for healthcare workers : video update. [videorecording]: Mount Kisco, N.Y. : Guidance Associates, 1992 1 videocassette (37 min.) : sd., col. ; 1/2 in. + 1 guide Unique Identifier : AIDSLINE AV/9200885A; Alpert LI; Committee
[AIDS o shiru.]: Tokyo : Kodansha, 1983 viii, 166, 15 p. : ill Unique Identifier : AIDSLINE CAT/9209700; Nishioka K
AIDS and adolescents : the time for prevention is now.: Washington, DC : Center for Population Options, [1987] 24 p Unique Identifier : AIDSLINE CAT/9209211; Haffner D
AIDS legal guide. 2nd ed., completely rev. and expanded.: New York, NY (666 Broadway, New York 10012) : Lambda Legal Defense and Education Fund, c1987 1 v. (various pagings) Unique Identifier : AIDSLINE CAT/9208835; Rubenfeld AR
[AIDS geht jeden an : Ergebnisse der Internationalen AIDS-Tagung im November 1986 in Berlin.]: Berlin : Kulturbuch, [1987] 92 p Unique Identifier : AIDSLINE CAT/9208463
[Solidaritat der Uneinsichtigen : Aktionstag 9. Juli 1988, Frankfurt a.M. : eine Dokumentation der Reden.]: Berlin : Deutsche AIDS-Hilfe, 1988 64 p. : ill (AIDS-Forum D.A.H. Sonderband) Unique Identifier : AIDSLINE CAT/9209992; Becker F
[SIDA : actualidades.]: Madrid : Biolecta, [1988?] 76 p. : ill (Publicaciones cientificas / Asociacion Espanola de Biopatologia Clinica) Unique Identifier : AIDSLINE CAT/9209599
AIDS : helping families cope : recommendations for meeting the psychosocial needs of persons with AIDS and their families : report to the National Institute of Mental Health, November 1987.: [Silver Spring, Md. : National Association of Social Workers, 1988] 10 p Unique Identifier : AIDSLINE CAT/9209702; Zlotnik JL
AIDS : the women. 1st ed.: San Francisco : Cleis Press, c1988 251 p Unique Identifier : AIDSLINE CAT/9209213; Rieder I; Ruppelt P
Policy guidelines for addressing HIV infection in the academic medical community : a report of the AAMC Committee on AIDS and the Academic Medical Center.: Washington, D.C. (One Dupont Circle, N.W., Washington 20036) : Association of American Medical Colleges, c1988 vii, 33 p Unique Identifier : AIDSLINE CAT/9209048
[AIDS und Jugendhilfe : jugendhilfe- und sozialrechtliche Situation.]: Berlin : Arbeitskreis zur Forderung von Pflegekindern, c1989 152 p Unique Identifier : AIDSLINE CAT/9209701; Munder J
Blood issues update : minimizing the risk of transfusion-associated HIV infection.: Blood Resources [Chicago, Ill.] : American Hospital Association, [c1989] 14 p (Hospital technology series. Special report, ISSN 0888-711X ; v. 3, no. 21 (Aug. 1989)) Unique Identifier : AIDSLINE CAT/9208844
AIDS and drug use : breaking the link.: New York, N.Y. : Citizens Commission on AIDS for New York City and Northern New Jersey, 1989 53 p Unique Identifier : AIDSLINE CAT/9209212
Building systems of care for children with HIV infection & their families, August 1989.: Development. Office of Maternal and Child Health Washington, DC : Association for the Care of Children's Health, c1989 19 p Unique Identifier : AIDSLINE CAT/9209726
[Materialien aus den Einrichtungen des Modells Betreuung und Beratung HIV-infizierter Drogenabhangiger an Drogenberatungsstellen : Konzeptionsbeschreibungen = Conceptions of AIDS-counseling in outpatient treatment centers.]: Munchen : Institut fur Therapieforschung, 1989 301 p. : ill (IFT-Berichte ; Bd. 51) Unique Identifier : AIDSLINE CAT/9209464; Schumann J
Research in perinatal medicine II : an interdisciplinary approach with special emphasis on epidemiology, hypoxia, and infections. 1. Aufl.: Berlin : Verlag Gesundheit, c1990- v. : ill Unique Identifier : AIDSLINE CAT/9209077; Grauel EL
Client satisfaction with inpatient and outpatient HIV/AIDS services in New South Wales.: [Sydney : The Bureau], 1990 51 p (State health publication ; no. (AIDS) 90-42) Unique Identifier : AIDSLINE CAT/9208882; Linder-Pelz S
AIDS education videotapes : an annotated bibliography for NADR project staff.: Rockville, Md. (5600 Fishers Lane, Room 9A-30, Rockville, 20857) : National Institute on Drug Abuse ; Bethesda, Md. (4600 East-West Highway, Suite 700, Bethesda, 20814) : NOVA Research Company, [1990] 85 p Unique Identifier : AIDSLINE CAT/9208834; Joseph L; Bentley S
[Leben und Sterben mit AIDS : Gesprache mit Patienten im Krankenhaus.]: Frankfurt/Main : VAS, c1990 214 p. : ill Unique Identifier : AIDSLINE CAT/9208626; Knupp B
AIDS law for mental health professionals : a handbook for judicious practice.: San Francisco, Calif. ; Berkeley, Calif. : AIDS Health Project : Distributed to the trade by Celestial Arts, c1990 xvii, 245 p Unique Identifier : AIDSLINE CAT/9208464; Wood GJ; Marks R; Dilley JW
Productive living strategies for people with AIDS.: New York : Haworth Press, 1990 227 p (Occupational therapy in health care, ISSN 0738-0577 ; v. 7, no. 2-4) Unique Identifier : AIDSLINE CAT/9208699; Johnson JA; Pizzi M
Culture and AIDS.: New York : Praeger, 1990 vi, 216 p Unique Identifier : AIDSLINE CAT/9208523; Feldman DA
The:Guide to resources on women and AIDS. Began in 1990.: Washington, DC : Center for Women Policy Studies, v Unique Identifier : AIDSLINE CAT/9208935
[Sexualerziehung als AIDS-Pravention : Arbeitsformen und Erfahrungen aus der Jugend- und Bildungsarbeit.]: Weinheim : Juventa, 1991 180 p Unique Identifier : AIDSLINE CAT/9209569; Stickelmann B
[Transfusion sanguine et SIDA.]: Paris : Editions Frison-Roche, c1991 78 p. : ill (Les Monographies de la Societe nationale de transfusion sanguine) Unique Identifier : AIDSLINE CAT/9209616; Lefrere J
[Tourismus, Prostitution, Aids.] 1. Aufl.: Zurich : Rotpunktverlag, 1991 146 p. : ill (Kleine Reihe Tourismus & Entwicklung ; Bd. 5) Unique Identifier : AIDSLINE CAT/9208773; Maurer M
[AIDSbekampfung in der Bundesrepublik Deutschland.] Aktualisierte 3. Aufl.: Bonn : Bundesministerium fur Gesundheit, 1991 99 p. : ill., ports (Das Bundesgesundheitsministerium informiert) Unique Identifier : AIDSLINE CAT/9208465
AIDS & HIV education : effective teaching strategies.: Portland, Me. (P.O. Box 658, Portland, 04104-0658) : J. Weston Walch, c1991 xvii, 153 p. : ill Unique Identifier : AIDSLINE CAT/9208462; Firkaly ST
AIDS, a strategy for nursing care. 3rd ed.: London : Arnold, 1991 viii, 279 p Unique Identifier : AIDSLINE CAT/9208836; Pratt RJ
National AIDS Clearinghouse conference calendar : a service of the Centers for Disease Control, National AIDS Information and Education Program. July 1991-: Rockville, Md. : The Clearinghouse, [1991- v Unique Identifier : AIDSLINE CAT/9208658
AIDS : prevention through education : a world view.: New York : Oxford University Press, 1992 382 p. : ill Unique Identifier : AIDSLINE CAT/9209703; Sepulveda Amor J; Fineberg HV; Mann JM
The:essential HIV treatment fact book.: New York : Pocket Books, c1992 xvi, 448 p. : ill Unique Identifier : AIDSLINE CAT/9208426; Pinsky L; Douglas PH; Metroka C
Families living with drugs and HIV : intervention and treatment strategies.: New York : Guilford Press, c1993 xvi, 368 p Unique Identifier : AIDSLINE CAT/9208549; Barth RP; Pietrzak J; Ramler M
SCRIPPS MEMORIAL HOSPITALS FOURTEENTH ANNUAL CANCER SYMPOSIUM. OCTOBER 22-24, 1990, LA JOLLA, CA: Scripps Memorial Hospitals Fourteenth Annual Cancer Symposium. October 22-24, 1990, La Jolla, CA, Thomas T Stevens Cancer Center of Scripps Memorial Hospitals, 264 p., 1990.. Unique Identifier : AIDSLINE ICDB/92680731; Anonymous; No affiliation given; The Scripps Memorial Hospitals Fourteenth Annual Cancer Symposium was held October 22-24, 1990, in La Jolla, CA. The five major sessions focused on hematology, evolution of multimodality therapy in gastrointestinal cancer, gynecological cancer, issues for the 1990s, breast cancer and AIDS. The following subjects were
RETROVIRAL ENV-MEDIATED LEUKEMOGENESIS (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 3, 1991.. Unique Identifier : AIDSLINE ICDB/92682362; Amanuma H; Watanabe N; Ikawa Y; Tsukuba Life Science Center, RIKEN, Tsukuba, Ibaraki 305, Japan; Friend spleen focus-forming virus (SFFV), originally isolated as one of the components of the Friend virus complex, is a member of the murine replication-defective type C retroviruses and induces acute, progressive erythroleukemia, called Friend disease, in adult mice. In the early stage, enhanced polyclonal prolifera
FACTORS IN THE DEVELOPMENT OF AIDS-RELATED LYMPHOMAS (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 9, 1991.. Unique Identifier : AIDSLINE ICDB/92682367; Broder S; NCI, Bethesda, MD; The association between AIDS and a spectrum of malignancies relates to chronic, profound defects in both cellular and humoral mechanisms of immune surveillance. Furthermore, viruses in addition to HIV may act directly or indirectly as cofactors to induce malignant transformation. As AIDS patients (pts) live longer i
ONCOGENES AND TUMOR-SUPPRESSOR GENES IN B-CELL LYMPHOMA PATHOGENESIS (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 13, 1991.. Unique Identifier : AIDSLINE ICDB/92682369; Dalla-Favera R; Dept. of Pathology, Coll. of Physicians and Surgeons, Columbia; Univ., New York, NY 10032; A group of B-cell malignancies including Burkitt s lymphoma (BL), L3-type acute lymphoblastic leukemia (ALL) and AIDS-associated non-Hodgkin s lymphoma (AIDS-NHL) are associated with specific chromosomal translocations involving the c-myc and immunoglobulin loci. The consequences o
MORPHOLOGICAL AND PHENOTYPICAL CHANGES IN EBV-POSITIVE LYMPHOBLASTOID CELLS INFECTED BY HIV-1 (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 14, 1991.. Unique Identifier : AIDSLINE ICDB/92682370; De Rossi A; Calabro ML; D'Andrea E; Panozzo M; Saggioro D; Mammano F; Gallo P; Roncella S; Ferrarini M; Chieco-Bianchi L; Inst. of Oncology, Univ. of Padova, Padova, Italy; To study the mode of HIV-1 infection in EBV-positive lymphoblastoid cell lines (LCLs), as well as the molecular and biological features of EBV- and HIV-1-infected B cells, 3 spontaneous LCLs from HIV-1 seropositive individuals and 2 LCLs derived in vitro by infection with supernatant from the B95.8 marmoset cell line,
CHARACTERIZATION OF C-TERMINAL TRUNCATIONS OF MOLONEY MULV PR65GAG (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 20, 1991.. Unique Identifier : AIDSLINE ICDB/92682376; Ernst S; Gorelick R; Rein A; Lab. of Molecular Virology and Carcinogenesis, ABL-Basic Res.; Program, Frederick, MD 21702; In an attempt to define functional domains of the gag precursor in Moloney MuLV, we have generated a number of mutants in the gag gene of an infectious molecular clone of this virus. We introduced a series of termination codons at different points in the p30ca and p10nc coding regions. Mammalian cells were transfected
HTLV-I TAX: ASSOCIATION WITH VIRUS TRANSMISSION AND LEUKEMOGENESIS (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 21, 1991.. Unique Identifier : AIDSLINE ICDB/92682377; Essex M; Korber B; Okayama A; Chen Y; Renjifo B; Lee TH; Tachibana N; Marlink R; Dept. of Cancer Biology, Harvard Sch. of Public Health, Boston,; MA 02115; The vast majority of HTLV-I-infected individuals make antibodies to the virus structural proteins, especially the env gene products gp46 and gp21. The high frequency with which these antibodies are detected facilitate blood antibody screening with a high degree of success. A smaller but distinct fraction of HTLV-I-inf
LYMPHOMA, AN OPPORTUNISTIC NEOPLASIA OF AIDS: A MULTIPARAMETER STUDY OF 111 CASES (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Disease. October 6-11, 1991, Padova/Venice, Italy, p. 37, 1991.. Unique Identifier : AIDSLINE ICDB/92682393; Ioachim HL; Dept. of Pathology, Lenox Hill Hosp., New York, NY; The incidence of lymphomas in HIV-infected persons has increased progressively since the beginning of the AIDS epidemic. In the United States there were, by 1989, 3000 cases of AIDS-associated non-Hodgkin s lymphomas (NHL) out of 100,000 cases of AIDS, an incidence of 3%, 60 times high
RETROVIRUS-INDUCED MURINE LYMPHOMA AND PARANEOPLASTIC SYNDROMES (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Disease. October 6-11, 1991, Padova/Venice, Italy, p. 42, 1991.. Unique Identifier : AIDSLINE ICDB/92682397; Jolicoeur P; Kay DG; Simard C; Huang M; Clinical Res. Inst. of Montreal, Montreal, Canada; Murine AIDS (MAIDS) shows many similarities with human AIDS. The etiologic agent of MAIDS is a defective retrovirus encoding a precursor gag protein (Pr60gag) which remains uncleaved. Helper-free stocks of this defective virus have been shown to induce disease. Using such helper-free stocks, we have found that the inf
GEOGRAPHY MORE THAN PATHOLOGY INFLUENCES THE LIMITED HTLV-I GENOMIC VARIABILITY (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Disease. October 6-11, 1991, Padova/Venice, Italy, p. 46, 1991.. Unique Identifier : AIDSLINE ICDB/92682398; Komurian F; Pelloquin F; Sonoda S; De The G; Pasteur Merieux, Marcy L'Etoile, France; In order to study the geographic and/or disease-associated variations of the HTLV-I genome, the ex-vivo DNA from seropositive peripheral blood lymphocytes of 4 Japanese patients (pts; 2 with tropical spastic paraparesis/HTLV-1 associated myelopathy [TSP/HAM] and 2 with adult T-cell leukemia [ATL]), 3 Caribbean pts (2
EXPRESSION AND BIOLOGICAL FUNCTION OF HUMAN ENDOGENOUS RETROVIRAL SEQUENCES (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Disease. October 6-11, 1991, Padova/Venice, Italy, p. 48, 1991.. Unique Identifier : AIDSLINE ICDB/92682399; Leib-Mosch C; Bachmann M; Brack-Werver R; Werner T; Erfle V; Hehlmann R; III Medizinische Klinik Mannheim der Universitat Heidelberg,; Wiesbadener Str. 7-11, D-6800 Mannheim 31, Germany; The human genome contains a variety of retroviral elements resembling mammalian leukemia viruses. Most of these sequences have been found to be related to primate and murine C-type viruses (BaEV, SSAV/GaLV, MuLV), to murine B-type viruses and A-type particles (MMTV, IAP), or to human T-cell lymphotropic viruses (HTLV)
THE EPIDEMIOLOGY OF HUMAN VIRUS-ASSOCIATED HEMATOLOGIC MALIGNANCIES (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Disease. October 6-11, 1991, Padova/Venice, Italy, p. 49, 1991.. Unique Identifier : AIDSLINE ICDB/92682400; Levine PH; Blattner WA; Viral Epidemiology Section, NCI, Bethesda, MD; The long history of virus-induced hematologic malignancies in a variety of animals strongly suggested that viruses would also be implicated in the etiology of human leukemia and lymphoma. Considerable clinical, epidemiologic and laboratory evidence links two viruses, Epstein-Barr virus (EBV) and human T-cell lymphotro
HTLV-I AS AGENT INDUCING GENETIC CHANGES IN INFECTED HUMAN LYMPHOCYTES (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Disease. October 6-11, 1991, Padova/Venice, Italy, p. 54, 1991.. Unique Identifier : AIDSLINE ICDB/92682404; Maruyama K; Fukushima T; Mochizuki S; Kawamura K; Miyauchi M; Dept. of Pathology, Chiba Cancer Center Res. Inst., Chiba 280,; Japan; HTLV-I is etiologically associated with adult T-cell leukemia (ATL). However, the relative low incidence of ATL among virus carriers in ATL-endemic areas suggests host and/or environmental cofactors for the development of ATL. In a search for host cofactors, members in 3 generations of a suspected carrier family were
DIDEOXYNUCLEOSIDES AS SPECIFIC ANTILEUKEMIC AGENTS IN LYMPHOBLASTIC DISEASE (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Disease. October 6-11, 1991, Padova/Venice, Italy, p. 55, 1991.. Unique Identifier : AIDSLINE ICDB/92682405; McCaffrey RP; Torres ER; Cronin K; Safran H; Dhawan R; Boston Univ. Medical Center, Boston, MA; Intense interest is now focused on the biological activity of 2 ,3 -dideoxynucleosides (ddNs) since their introduction as antiretroviral agents in HIV-infected individuals. We have noted that the lymphoblastic leukemia-associated DNA polymerase, terminal deoxynucleotidyl transferase (TdT), like HIV reverse transcripta
STUDIES ON THE MECHANISMS OF HTLV-I LEUKEMOGENESIS (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 82, 1991.. Unique Identifier : AIDSLINE ICDB/92682422; Saggioro D; Forino M; Chieco-Bianchi L; Inst. of Oncology, Univ. of Padova, Padova, Italy; Human T-lymphotropic virus type I (HTLV-I) gene expression in vivo and in vitro is quite restricted, even if HTLV-I LTR promoter/enhancer sequences are susceptible to different cellular and viral stimuli. The mechanisms of viral latency remain to be elucidated, but it is believed that host cellular factors are involve
NATURAL HYBRIDOMAS (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 88, 1991.. Unique Identifier : AIDSLINE ICDB/92682428; Sinkovics JG; Cancer Inst., St. Joseph's Hosp., Tampa, FL; Knowledge and expertise concerning artificially constructed hybridomas (the Kohler-Milstein technology and its modifications) rapidly increase. Information on naturally occurring hybridomas and on the role of these formations in the course and outcome of malignant disease remains in a most retarded state. The purpose
RETROVIRUS RECEPTORS EXPRESSED ON HUMAN CELLS (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 102, 1991.. Unique Identifier : AIDSLINE ICDB/92682440; Weiss RA; Chester Beatty Laboratories, Inst. of Cancer Res., London SW3; 6JB, UK; Eight distinct receptors have been delineated on human cells for 25 strains of C-type and D-type oncoviruses and lentiviruses. Some receptors are highly expressed in many cell lineages, whereas others are more restricted. The gene determining the receptor for the human and simian T-cell leukemia viruses resides on hum
HIV-1-INDUCED NEUROPATHY: HIV NEF EXHIBITS STRUCTURAL AND FUNCTIONAL SIMILARITIES TO PEPTIDES TARGETED TO ION CHANNELS (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 103, 1991.. Unique Identifier : AIDSLINE ICDB/92682441; Werner T; Ferroni S; Saermark T; Brack-Werner R; Banati RB; Mager R; Steinaa L; Keutzberg G; Erfle V; GSF-Center for Environmental and Health Res., Munchen, Germany; The molecular pathways leading to the HIV-mediated neuropathies are not well understood. Possibilities such as the neuronal killing of gp120 (Brenneman et al, 1990), the secretion of neurotoxic substances by HIV-1-infected macrophages (Giulian et al, 1990) or the neurotoxic activity of HIV-1 tat (Sabatia et al, 1991)
MOLECULAR STUDIES ON HTLV-1 GENE EXPRESSION ASSOCIATED WITH ADULT T-CELL LEUKEMIA AND TSP/HAM (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 106, 1991.. Unique Identifier : AIDSLINE ICDB/92682443; Yoshida M; Fujisawa J; Dept. of Cellular and Molecular Biology, Inst. of Medical Sci.,; Univ. of Tokyo, Shirokanedai, Minato-ku, Tokyo 108, Japan; Human T-cell leukemia virus type 1 (HTLV-1) is an etiologic agent of adult T-cell leukemia (ATL), and is also associated with tropical spastic paraparesia. Viral replication and gene expression are regulated by at least two viral transacting regulators, tax and rex, whose functions are also involved in development of
ANALYSIS OF CADMIUM-INDUCED MUTATIONS ALTERING CONDITIONALLY DEFECTIVE CELL TRANSFORMATION BY MUSVTS110 (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 33:A686 1992. Unique Identifier : AIDSLINE ICDB/92683212; Heckman CA; Murphy EC Jr; UT M.D. Anderson Cancer Center, Houston, TX 77030; A number of metals, including cadmium and nickel, are established carcinogens, but the mechanism by which they damage DNA is not well understood. For example, both are strongly clastogenic, but have proved negative or weakly mutagenic in prokaryotic assays. To study damage by carcinogens to mammalian genes, we develop
INHIBITION OF TUMOR PROMOTER-MEDIATED OXIDATIVE PROCESSES BY CAFFEIC ACID PHENETHYL ESTER (CAPE) (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 33:A967 1992. Unique Identifier : AIDSLINE ICDB/92683493; Frenkel K; Wei H; Bhimani R; Ye J; Huang MT; Ferraro T; Conney AH; Grunberger D; Dept. of Environmental Medicine, New York Univ. Medical Center,; New York, NY 10016; CAPE, a product isolated from the propolis of honey-bee hives, is cytotoxic to tumor and virally transformed, but not to normal cells. However, its mode of action is unknown. Since tumor promoters induce oxidative processes in vitro and in vivo, we evaluated the effect of CAPE upon these processes. We found that CAPE
POTENTIATION OF CYTOTOXICITY OF KAPOSI'S SARCOMA RELATED TO IMMUNODEFICIENCY SYNDROME (AIDS) BY LIPOSOME ENCAPSULATED DOXORUBICIN (LED) (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 33:A1483 1992. Unique Identifier : AIDSLINE ICDB/92684004; Masood R; Rahman A; Husain SR; Salahuddin SZ; Gill P; Dept. of Medicine, Norris Cancer Center, Univ. of Southern; California, Los Angeles, CA; Kaposi s sarcoma is an independent criteria for the diagnosis of AIDS and develops in nearly 30% of all cases. Current chemotherapy regimens are associated with substantial toxicity, particularly bone marrow suppression, which, thus, limits their use in many patients. To develop new modalities of treatment, LED was pr
HUMAN HERPES VIRUS-6 (HHV-6) INCREASES EXPRESSION OF HUMAN PAPILLOMA VIRUS (HPV) GENES IN MALIGNANT AND NONMALIGNANT CERVICAL CELLS (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 33:A2341 1992. Unique Identifier : AIDSLINE ICDB/92684897; DiPaolo JA; Chen MC; Corbellino M; Ablashi DV; NCI, Bethesda, MD 20892; Evidence associates HPVs as 1 factor in cervical cancer. This study examined the relationship between expression of HPV-16 or HPV-18 genes and HHV-6. HHV-6 transactivates HIV-1 LTR (HIV-1 has been found in cervicitis) suggesting that HHV-6 infection may affect other viruses such as cervical HPVs. Cervical HPV immortal
ACTIVATION OF PHORBOL DIESTER-INDUCED HUMAN IMMUNODEFICIENCY VIRUS GENE EXPRESSION IS INHIBITED BY ET-18-OCH3 AND RELATED ETHER LIPID ANALOGS (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 33:A2487 1992. Unique Identifier : AIDSLINE ICDB/92685008; Daniel LW; Civoli F; Smitherman PK; Wake Forest Univ., Winston-Salem, NC 27157-1016; The phorbol diester tumor promoter (TPA) stimulates protein kinase C (PKC), mimics the effects of growth factors on cell proliferation and stimulates replication of latent human immunodeficiency virus (HIV). Activation of the transcription factor NF-kB is associated with these effects. ET-18-OCH3 (1-O-octadecyl-2-O-me
NUCLEOSIDE TRANSPORT AND METABOLISM IN HUMAN IMMUNE CELLS (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 33:A2355 1992. Unique Identifier : AIDSLINE ICDB/92685077; Chan TC; Shaffer L; Redmond R; Pennington KL; Dept. of Physiology and Pharmacology, Purdue Univ., W. Lafayette,; IN 47907; There is a broad-specificity nucleoside transporter on mammalian cell membranes which is responsible for the facilitated uptake of endogenous nucleosides. It is unclear whether this nucleoside transport system is present in human immune cells, and whether any of the anti-HIV dideoxynucleosides are substrates of this f
2,3-DIDEOXYRIBOSE-1-PHOSPHATE, A MAJOR METABOLITE OF THE ANTI-HIV DIDEOXYPURINE DDINO (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 33:A2376 1992. Unique Identifier : AIDSLINE ICDB/92685086; Hao Z; Ford H; Ahluwalia G; Yeh H; Barchi J; Johns DG; Cooney DA; Lab. of Medicinal Chemistry, NCI, Bethesda, MD 20892; Purine nucleoside phosphorylase (PNP) cleaves dideoxyinosine to dideoxyribose-1-phosphate (ddR1P) and hypoxanthine. Previous attempts to identify ddR1P chromatographically have failed; we now trace that failure to the notable acid and heat lability of this sugar: its t1/2 at pHs 5, 7 and 10 being 1 min, 120 min and gr
PHARMACOKINETICS OF AN 'ANTISENSE' PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDE AFTER SUBCUTANEOUS INFUSION IN MICE (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 33:A3178 1992. Unique Identifier : AIDSLINE ICDB/92685713; Bigelow JC; Chrin LR; Mathews LA; McCormack JJ; Dept. of Pharmacology, Univ. of Vermont, Burlington, VT 05405; NSC 624958 is a phosphorothioate oligodeoxynucleotide (S-ODN; 27-mer) with a base-sequence antisense to the rev region of the HIV genome. Results of earlier pharmacokinetic studies, after iv administration to mice, suggested that continuous sc infusion might be an efficient approach to delivery of this drug. The maj
ANALYTICAL AND PHARMACOKINETIC STUDIES WITH AN ANTIVIRAL SULFONE DERIVATIVE (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 33:A3179 1992. Unique Identifier : AIDSLINE ICDB/92685714; Bigelow JC; Chrin LR; Mathews LA; McCormack JJ; Dept. of Pharmacology, Univ. of Vermont, Burlington, VT 05405; NSC 624231 is a phenyl sulfone derivative (PS) that has demonstrated activity against HIV in vitro. Detection and determination of PS in biological fluids is accomplished by HPLC analysis, using an ODS column and isocratic elution with acetonitrile/water (50/50); biological fluids are processed with acetonitrile prior
LYMPHOMA INDUCTION BY HUMAN B CELLS IN SCID MICE (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 2, 1991.. Unique Identifier : AIDSLINE ICDB/92682361; Amadori A; Veronese ML; Mazza MR; Zamarchi R; D'Andrea E; Menin C; Del Mistro A; Leszl A; Panozzo M; Chieco-Bianchi L; Inst. of Oncology, Univ. of Padova, Padova, Italy; The severe combined immunodeficiency (SCID) mouse represents a new interesting model for both immunologic and oncologic investigations. Several workers observed the high frequency with which SCID mice, injected ip with peripheral blood mononuclear cells (PBMC) from Epstein-Barr virus (EBV)-positive (+) donors, develop
TREATMENT OF NON-HODGKIN'S LYMPHOMAS (NHL) AND HUMAN IMMUNODEFICIENCY VIRUS (HIV) (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Diseases. October 6-11, 1991, Padova/Venice, Italy, p. 30, 1991.. Unique Identifier : AIDSLINE ICDB/92682386; Gisselbrecht C; Tirelli U; Oksenhendler E; Gabarre J; Gastaldi R; Farcet JP; Lepage E; Raphael M; Monfardini S; GELA-GICAT, Inserm-CNR study, Hopital Saint-Louis, Paris 75010,; France; NHL HIV patients (pts) have a poor prognosis related to lymphoma and HIV infection. To evaluate whether a subset of pts could be treated with intensive chemotherapy, a pilot study was initiated based on a clinical stratification. Pts were stratified in 2 risk groups: group A, no opportunistic infection (OI) and perfor
CHARACTERIZATION OF EBV-POSITIVE LYMPHOBLASTOID B CELL LINES ORIGINATED FROM HIV-SEROPOSITIVE PATIENTS WITH AND WITHOUT LYMPHOMAS (MEETING ABSTRACT): Fifteenth Symposium of the International Association for Comparative Research on Leukemia and Related Disease. October 6-11, 1991, Padova/Venice, Italy, p. 78, 1991.. Unique Identifier : AIDSLINE ICDB/92682419; Roncella S; Francia Di Celle P; Cutrona G; Foa R; Carbone A; Rowe M; Ferrarini M; Istituto Nazionale per la Ricerca sul Cancro, Laboratorio di; Immunologia Clinica, Genova, Italy; EBV-positive B-cell lines (LCLs) were originated spontaneously,in vitro, from HIV-seropositive patients (pts). Particular caution was taken to ensure that the LCLs were originated by an in vitro expansion of in vivo latently infected B cells rather than by B cells infected by the EBV released in vitro. The LCLs from H
EFFECT OF RETROVIRAL LONG TERMINAL REPEAT (LTR)-CONTAINING TRANSGENES ON THYMIC LYMPHOMA INDUCTION IN FVB/N MICE NEONATALLY TREATED WITH AZACYTIDINE (MEETING ABSTRACT): Proc Annu Meet Am Assoc Cancer Res; 33:A1100 1992. Unique Identifier : AIDSLINE ICDB/92683623; Wang TH; Yang WK; Saavedra HI; Yang DM; Popp DM; Hoyt PR; Ch'ang LY; Yang MY; Biology Div., Oak Ridge Natl. Lab., Oak Ridge, TN 37831; In an attempt to activate the ecotropic LTR-containing transgene in FVB/N mice, we have found that neonatal injections of azacytidine can cause high incidence of thymic lymphomas in the transgenic mice at 3 to 7 mo of age, with massive involvement of thymus and other lymphoid tissues, as well as, tumor-cell infiltrati
Detection of Rhodococcus equi in tissues of HIV-infected patients by in situ hybridization (ISH) using a biotinylated probe.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:82 (abstract no. D-26). Unique Identifier : AIDSLINE ASM91/0010292; Brown J; McNeil M; Greer P; Lasker B; Bluth R; Schaffner W; Jahre J; Oblack D; Centers for Disease Control, Atlanta, GA.U Zaki S; R. equi, an emerging pathogen in severely immunocompromised patients, has recently been reported to cause disseminated fatal infections in HIV-infected patients. However, the diagnosis may be very difficult; definitive microbiologic identificatio may take 2 weeks and no fluorescein-labeled antibody test exists for R.
Detection and isolation of Mycoplasma fermentans from urine of patients with AIDS.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:134 (abstract no. G-4). Unique Identifier : AIDSLINE ASM91/0020292; Dawson MS; Wang R; Hayes M; Armstrong D; Budzko D; Lo SC; Kundsin R; ARP, Armed Forces Institute of Pathology, Washington, D.C.; Mycoplasmas of the M. fermentans species, a rarely identified urogenital mycoplasma was shown to infect the kidneys of HIV positive patients with AIDS-associated nephropathy. We set out in this study to 1) detect M. fermentans in the urine sediments of HIV positive patients utilizing the polymerase chain reaction (PCR
Selective detection of Mycoplasma fermentans using the polymerase chain reaction (PCR).: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:134 (abstract no. G-5). Unique Identifier : AIDSLINE ASM91/0030292; Wang R; Hu W; Dawson MS; Lo SC; Shih JW; ARP, Armed Forces Institute of Pathology, Washington, DC.; We previously developed a PCR assay for M. fermentans (incognitus strain). The assay using primer pairs RS47 and RS49 however failed to detect many mycoplasmas of the M. fermentans species, including PG-18, the prototype of M. fermentans and new clinical isolates from patients with AIDS. Recently, we reported the pres
Use of DNA probes to further characterize Mycobacterium avium complex (MAC) strains isolated from patients with AIDS.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:145 (abstract no. U-20). Unique Identifier : AIDSLINE ASM91/0040292; Yajko DM; Sanders CA; Kirihara JM; Nassos PS; Hadley WK; Gonzalez PC; Univ. of California, San Francisco Gen. Hosp. Med. Ctr., San; Francisco, CA.; Thirty stains of MAC isolated from AIDS patients (25 from blood, 4 from sputum, 1 from bile), that were previously characterized with regard to serotype and antimicrobial susceptibility patterns, were tested for reactivity with the 3 DNA probes in the SNAP (TM) (Syngene Inc, San Diego, CA) M. avium complex kit. The pr
Humoral response to Mycobacterium avium complex disease in AIDS and non-AIDS populations.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:148 (abstract no. U-33). Unique Identifier : AIDSLINE ASM91/0050292; Morris S; Armoa G; Rouse D; Chaparas S; Center for Biologics Evaluation and Research, FDA, Bethesda, MD; 20892.; Disease resulting from Mycobacterium avium, M. intracellulare complex (MAC) infections has become a serious complication for patients with acquired immmunodeficiency syndrome (AIDS). Because the detection of MAC disease is difficult and time-consuming, we have examined sera taken from AIDS and non-AIDS patients with M
Comparison of lymphocyte populations in patients infected with M. avium possessing varying colony morphology.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:148 (abstract no. U-34). Unique Identifier : AIDSLINE ASM91/0060292; Tassell SK; King B; Measel JW; Weis SE; Barrow WW; Biotherapeutics, Arlington, Texas.; The Mycobacterium avium complex is a group of opportunistic pathogens clinically important in AIDS patients and whose mechanisms of pathogenicity have not been defined. Lymphocyte populations were enumerated in a group of HIV-negative patients to identify modified immune parameters associated with chronic M. avium inf
Cost effective idenification of Mycobacteria isolated from blood.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:148 (abstract no. U-35). Unique Identifier : AIDSLINE ASM91/0070292; Kaminski DA; Weymouth LA; Casiano-Colon AE; Univ. of Rochester Med. Ctr., Rochester, NY.; Mycobacterium avium complex (MAC) is a common cause of disseminated infection and mycobacteremia in AIDS patients. A total of 994 blood specimens in Dupont Isolator tubes were received in our laboratory for mycobacterial culture during one year (8/89-7/90). 127 cultures (12.8%) from 26 patients were positive for mycob
Characterization of Mycobacterium avium isolated from multiple sites in AIDS patients.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:149 (abstract no. U-42). Unique Identifier : AIDSLINE ASM91/0080292; Yakrus M; Brown S; Smith C; Kilburn J; Good RC; Butler W; Centers for Disease Control, Atlanta, GA.; Mycobacterium avium can be isolated from a variety of sites in AIDS patients who have disseminated infection. We characterized isolates from multiple sites in 21 AIDS patients to determine if individual infections are due to one or more strains. Specimen sources included blood, sputum, stool, bronchial-alveolar lavage
In vitro synergistic inhibitory effect of ethambutol and vancomycin aginst Mycobacterium avium complex (MAC).: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:151 (abstract no. U-56). Unique Identifier : AIDSLINE ASM91/0090292; Berlin OG; Lang HL; Shum AC; UCLA Medical Center, Los Angeles, CA 90024-1713.; Fifty clinical isolates of MAC, from AIDS and non-AIDS patients (32 M. avium and 18 M. intracellulare), were tested against Ethambutol (EB) and Vancomycin (VA) individually and in combination. Commercially available antimicrobial disks (BBL Microbiology Systems, Cockeysville, MD) of EB (25 ug) and VA (30 ug) were plac
Therapeutic efficacy of Benzoxazinorifamycins against Mycobacterium avium complex infection induced in mice and rabbits.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:152 (abstract no. U-59). Unique Identifier : AIDSLINE ASM91/0100292; Saito H; Tomioka H; Sato K; Hidaka T; Shimane Medical University, Izumo, Japan.; Newly synthesized rifamycin derivatives, benzoxazinorifamycins (KRMs; Kanegafuchi Chemical Ind. Co., Osaka, Japan ), have a markedly improved in vitro activity against various mycobacteria including M.avium complex (MAC), and their MICs against most strains were more than 64 times lo
MICs and MBCs of clarithromycin for M. avium isolated from AIDS patients.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:152 (abstract no. U-60). Unique Identifier : AIDSLINE ASM91/0110292; Heifets L; Comstock D; Lindholm-Levy P; Natl. Jewish Ctr. Immunol. Respir. Med., Denver, CO.; Clarithromycin is the only antimicrobial agent that has been tested in a controlled clinical trial for the therapy of M. avium infection. This trial, conducted in France , has shown the ability of this drug to eliminate M. avium from the blood of AIDS patients. The aim of our study
Trends and correlations in Mycobacteria in an inner-city hospital 1983-89.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:153 (abstract no. U-65). Unique Identifier : AIDSLINE ASM91/0120292; Peters B; Olopoenia L; Frederick W; Delapenha R; Howard Univ. Hosp. and Col. of Med. Washington, DC.; Positive mycobacterial cultures (PMC) from 1983-89 were reviewed and compared. Total number of PMC, % of PMC due to M.tuberculosis (MTB), M.avium complex (MAC) and other mycobacteria (OM), mean age of patients (pts) and % of men with PMC were: TABULAR DATA, SEE ABSTRACT VOLUME. Yearly increases occurred in total PMC,
Sepsis caused by Mycobacterium tuberculosis (MTB) in Spanish patients.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:154 (abstract no. U-70). Unique Identifier : AIDSLINE ASM91/0130292; Mattar S; Drobnic L; Morta M; Hernaandez P; Saanzhez E; Urbina D; Gimeno O; Postgrado de Microbiologaia, Universidad de Cartagena and; Hospital Del Mar, Barcelona, Spain.; Mycobacteremia is rarely detected despite hematogenous spread of disease during dissemination, because culturing for MTB has not become a routine Laboratory test. In this work we studied 25 AIDS patients admitted to the Hospital with unknown fever. An HIV negative patient was also included in the study. Blood samples
Development of an escherichia coli culture and fermentation process for PL-regulated expression of HIV-1 protease.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:190 (abstract no. H-212). Unique Identifier : AIDSLINE ASM91/0140292; Strakalaitis N; Hoogerheide J; Mott J; Tomich CS; Brunner D; Vanzanten R; The Upjohn Co., Kalamazoo, MI.; HIV-1 protease consists of 99 amino acids and shares activity and homology with aspartyl proteases, such as pepsin and renin. Specific protease inhibitors block viral replication and infection, and are potential therapeutic agents for the treatment of AIDS. To date,
Virucidal efficacy of various lens cleaning and disinfecting solutions on HIV-I contaminted contact lenses.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:334 (abstract no. T-2). Unique Identifier : AIDSLINE ASM91/0150292; Amin RM; Dean MT; Poiesz BJ; Zaumetzer LE; VA Medical Center and SUNY Health Science Center at Syracuse, NY.; The isolation of Human Immunodeficiency Virus (HIV-I) from tears, cornea, conjuctival epithelium and contact lenses has raised significant concern about the possible transmissibility of this virus during routine opthalmologic procedures such as trial contact lens fittings. Because of this, we sought to determine viruc
A sensitive microculture assay for the isolation of human immunodeficiency virus type 1 (HIV-1) from blood leukocytes.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:334 (abstract no. T-3). Unique Identifier : AIDSLINE ASM91/0160292; Erice A; Sannerud KJ; Balfour Jr HH; Leske VL; Dept. of Lab Med. & Pathology. Univ. of Minnesota Hosp. and; Minnesota ACTU. Minneapolis, MN.; HIV-1 has been isolated by coculturing 10x10(6) peripheral blood mononuclear cells (PBMC) from HIV-1 seropositive patients (pospts) with 5x10(6) PHA-stimulated PBMC from normal donors. Because pospts contain high numbers of HIV-1 infected PBMC, we developed a microculture method (MCX) using fewer PBMC. 1x10(6) PBMC fr
Detection of HIV-1 transcropts and HIV-1 viremia by reverse-transcription polymerase chain reaction.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:334 (abstract no. T-4). Unique Identifier : AIDSLINE ASM91/0170292; Bagnarelli P; Clementi M; Menzo S; Pauri P; Valenza A; Varaldo PE; Manzin A; Institutes of Microbiology, University of Ancona and Trieste -; Italy.; Aim of the research has been to define a molecular boundary between virus latency and active replication phase in course of HIV-1 infection. For this purpose PBLs and plasma from seropositive subjects were checked for the presence of HIV-1 specific transcipts and genomic RNA (HIV-1 viremia), respectively, using revers
PCR detection of integrated HIV-1 DNA in peripheral blood lymphocytes (PBL) from infected individuals at various stages of disease.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:334 (abstract no. T-5). Unique Identifier : AIDSLINE ASM91/0180292; Casareale D; Kung K; Dragon B; Giron J; Wolfe L; PCR Division, Roche Diagnostic Systems Inc, Fair Lawn, NJ.; While available evidence indicates that viremia in HIV-infected individuals is not persistent and is influenced by neutralizing antibody levels, integrated HIV-1 DNA has been shown to be a more consistent marker for prior infection. Although most previous studies did not include terminal AIDS patients, in this study i
PCR sterilization: an evaluation of two methods.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:335 (abstract no. T-6). Unique Identifier : AIDSLINE ASM91/0190292; Frelow MM; Louie P; Rodgers G; Madej R; Roche Diagnostics Research, 1145 Atlantic Ave., Alameda, CA; 94501.; Contamination of polymerase chain reaction (PCR) reactions and DNA samples with previously amplified DNA products (amplicon) has become a source of concern. PCR sterilization involves the modification of elimination of amplicon contaminants. We have evaluated two methods for PCR sterilization: a post-PCR sterilization
Detection of HIV-1 and -2 infection by the polymerase chain reaction (PCR).: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:335 (abstract no. T-7). Unique Identifier : AIDSLINE ASM91/0200292; Brown V; Dela Torre N; Moore J; Fridlund C; George J; Schochetman g; Brattegaard K; Boateng E; Decock K; Ou CY; Centers for Disease Control. Atlanta, GA.; PCR is an effective diagnostic tool for the identification of HIV-1 infection from clinical specimens. Due to the presence of cross-seroreactivity and mixed infections in regions of West Africa, the use of PCR to differentiate HIV-1 and -2 infections is important. However, difficulty is encountered in designing HIV-2
Amplification of HIV gag and env genes from kidney biopsy archival material.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:335 (abstract no. T-8). Unique Identifier : AIDSLINE ASM91/0210292; Ferreira-Centeno A; Kimmel PL; Rodriguez ER; Farkas-Szallasi T; Abraham AA; Garrett CT; Departments of Pathology and Medicine, George Washington; University Medical Center, Washington D.C.; Patients infected with the HIV virus may develop a form of renal disease known as HIV-associated nephropathy. To elucidate whether HIV could cause renal disease directly, we first examined renal tissue from 8 patients with kidney disease to determine if HIV genome was present. DNA from paraffin sections of renal biops
PCR analysis of HIV protease sequences from lab isolates.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:335 (abstract no. T-9). Unique Identifier : AIDSLINE ASM91/0220292; Fontenot G; Cohen C; Robinson J; Johnston K; Luftig R; Dept. of Microbiology, Immunology and Parasitology; LSU Medical; Center, New Orleans, LA.; HIV replication involves a limited proteolysis of viral precursor proteins containing gag and gag-pol sequences by a specific viral protease. In order to characterize the HIV protease, sequences from: a) 5 patient isolates in New Orleans, b) 4 established strains grown in our lab, c) 10 strains in the Los Alamos datab
Primer extension analysis of the inhibition of purified HIV-1 reverse transcriptase by the TIBO analog, R82150.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:335 (abstract no. T-10). Unique Identifier : AIDSLINE ASM91/0230292; Noll GJ; Frank KB; Connell EV; Sim IS; Roche Res. Ctr., Nutley, NJ.; Recent studies have indicated that members of the TIBO (tetrahydro-imidazo [4,5,1-jk] [1,4]-benzodiazepin-2 (1H)-thione) family of compounds inhibit HIV-1 RT. We have used primer extension analysis to study the mechanism of action of one derivative. An in vitro transcript was made from a DNA construct containing the H
Development of an automated enzyme immunoassay for the detection and quantitation of human immunodeficiency virus (HIV) antigen.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:335 (abstract no. T-11). Unique Identifier : AIDSLINE ASM91/0240292; Bauer R; Cole C; Baxter Diagnostics, Mundelein, IL.; HIV is the etiologic agent of AIDS, and the demonstration of HIV antigen in patient serum or plasma samples correlates with disease progression. Further, monitoring HIV antigen levels during therapeutic intervention correlates with the therapeutic s efficacy. Thus, a method for the rapid and accurate quantitation of H
Comparison of whole cell viral lysate (VL), synthetic peptide (SP), and recombinant protein (RCP) EIA's for detection of HIV-1 antibody.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:336 (abstract no. T-12). Unique Identifier : AIDSLINE ASM91/0250292; Johnson JE; Univ. Kentucky and VA Medical Center, Lexington, KY.; Five hundred seventy five serum specimens received for HIV-1 antibody determination were tested prospectively by EIA using a whole cell viral lysate (Genetic Systems Corp.) a synthetic peptide ( United Biomedical , Inc.) and a recombinant protein (Syva Co.). Concordance of all 3
Human immunodeficiency virus type 1 (HIV-1) antibody testing: analytic sensitivity and analytic specificity of enzyme immunoassay (EIA) results.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:336 (abstract no. T-13). Unique Identifier : AIDSLINE ASM91/0260292; Cross GD; Schalla WO; Blumer SO; Hearn TL; Valdiserri RO; Centers for Disease Control, Atlanta, GA.; Laboratories enrolled in the CDC model performance evaluation program for HIV-1 antibody testing reported EIA results after testing HIV-1 performance evaluation samples sent them in February and May 1990. The panels contained 10 individual samples that were either HIV-1 antibody-negative, antibody-positive, or indeter
Evaluation of synthetic peptide EIA for HIV-1 and HIV-2 for use in seroprevalence studies.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:336 (abstract no. T-14). Unique Identifier : AIDSLINE ASM91/0270292; George R; Granade T; Holloman D; Schochetman G; De Cock K; Gayle H; Phillips S; Centers for Disease Control, Atlanta, GA.; Three commercial synthetic peptide EIAs (PEIA) for HIV-1 and -2 antibody and a combined HIV-1/2 EIA were evaluated for their sensitivity and specificity as compared to FDA-licensed whole virus lysate EIAs (VEIA) and Western blots (WB). A panel of 107 HIV-1 weakly reactive sera, 19 HIV-1 seroconversion panels, and 158
Opportunistic infections (OI) in patients with AIDS in central United States: differences from from large metropolitan areas.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:336 (abstract no. T-15). Unique Identifier : AIDSLINE ASM91/0280292; Woods GL; Goldsmith JC; University of Nebraska Medical Center (UNMC), Omaha, NE.; AIDS cases from the central United States in areas with less than or equal to 500,000 population are increasing; thus an awareness of the prevalence of infections in more rural locations is important to disease management and prevention. Of 102 adults (94 males, 8 females) with AIDS ca
Characterization of a murine monoclonal antibody to the transmembrane glycoprotein of human immunodeficiency virus type 2.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:336 (abstract no. T-16). Unique Identifier : AIDSLINE ASM91/0290292; Granade T; Parekh B; Phillips S; Pau C; George JR; Centers for Disease Control, Atlanta, GA.; Monoclonal antibodies (MAB) were developed to study the viral proteins of human immunodeficiency virus type 2 (HIV-2). Mabs were produced with standard techniques using whole viral lysate as immunogens. One MAB (1H5) displayed strong reactivity to the transmembrane glycoprotein gp41 by EIA and by Western blot analysis
Inhibition of HIV-2 induced syncytia formation by a monoclonal antibody.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:336 (abstract no. T-17). Unique Identifier : AIDSLINE ASM91/0300292; Rodriguez AE; Kraiselburd EN; Diaz AM; Dept. of Microbiology & Med. Zoology, Univ. of Puerto Rico, San; Juan, PR.; The purpose of this work was to test the ability of monoclonal antibodies to inhibit in vitro HIV-2 induced syncytia formation of CEMss cells. We have isolated one clone (K7.1) that produces monoclonal antibodies against HIV-2 antigens. These IgG antibodies were concentrated by injecting cloned cell into BALB/c mice,
HIV induction of lymphocyte-derived corticotropin.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:337 (abstract no. T-18). Unique Identifier : AIDSLINE ASM91/0310292; Hashemi FB; Hughes TK; Smith EM; Departments of Microbiology and Psychiatry, University of Texas; Medical Branch, Galveston, TX 77550.; AIDS is a disease which affects the immune, nervous and endocrine systems. Normally, cellular and humoral linkages exist among these three systems. We investigated HIV s role in altering these linkages via abnormal induction of ACTH by lymphocytes. Human peripheral blood mononuclear leukocytes (PBML) and cultured t-ly
Cytomegalovirus infection accelerates the rate of immunosuppression in HIV-infested subjects.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:337 (abstract no. T-19). Unique Identifier : AIDSLINE ASM91/0320292; Smith KO; Ludwig MJ; Ratner JJ; Boswell RN; The University of Texas Health Science Center at San Antonio and; the Wilford Hall USAF Hospital, San Antonio, TX.; This is the fourth year of a study to determine whether cytomegalovirus ( CMV ) might be a cofactor with the human immunodeficiency virus (HIV) to accelerate immunosuppression. Over seven hundred healthy HIV-seropositive U.S. A
Expression of CD4 and HIV-specific immunoglobulins in bacteria.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:337 (abstract no. T-20). Unique Identifier : AIDSLINE ASM91/0330292; McCallus DE; Ugen KE; Williams WV; Weiner DB; The Wistar Insitute and the University of Pennsylvania,; Philadelphia, PA.; The production of eucaryotic proteins in bacteria has been a major goal for many laboratories. We are attempting to express several HIV-related proteins in Escherichia coli using a pUC19-based vector. A bacterial plasmid (pDAB) was constucted containing the leader sequence for the pectate lyase protein of Erwinia caro
Detection of HTLVI DNA sequences by PCR in Multiple Sclerosis patients.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:338 (abstract no. T-27). Unique Identifier : AIDSLINE ASM91/0340292; Ferrante P; Mancuso R; Caputo D; Guerini F; Achilli G; Cattaneo E; Landini V; Zella D; Bertazzoni U; Don Gnocchi MS Ctr., Inst. Med. Microb. Univ. Milan, Italy.; In order to study the involvment of HTLVI in multiple sclerosis (MS) we searched HTLVI DNA in a group of 8 MS patients positive for antibody toward HTLVI antigens, in 18 MS patients without antibodies and in 8 healty controls. On the fresh PBL of all the subjects we performed PCR analysis using primers of the tax/rex
Assesment of human T-lymphotropic virus type I/II antibody testing.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:338 (abstract no. T-29). Unique Identifier : AIDSLINE ASM91/0350292; Blumer S; Schalla W; Cross D; Hearn T; Howerton D; Valdiserri R; Centers for Disease Control, Atlanta, GA.; In 3 performance evaluation surveys (September 1989, April and July, 1990), panels consisting of HTLV-I/II antibody-positive and antibody-negative plasma samples were mailed to more than 350 candidate reference and participant laboratories voluntarily enrolled in the CDC Model Performance Evaluation program for HTLV-I
Molecular characterization of recombinant BIV gag proteins.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:339 (abstract no. T-31). Unique Identifier : AIDSLINE ASM91/0360292; Atkinson RB; Liu ZQ; Wood C; University of Kansas, Lawrence, KS.; Bovine Immunodeficiencylike virus (BIV) is a member of the lentivirus subfamily of retroviruses. BIV has been shown to be antigenically related to HIV-1 from human, STLV-III from monkey, EIAV from horse, and CAEV from sheep. The purpose of our studies is to generate several recombinant BIV gag proteins for use in immu
Antigenic relatedness between HIV-1 and ovine progressive pneumonia virus.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:339 (abstract no. T-32). Unique Identifier : AIDSLINE ASM91/0370292; Maslak DM; Schmerr MJF; National Animal Disease Center, Agricultural Research Service,; USDA, Ames, IA.; Ovine progessive pneumonia virus (OPPV) is a lentivirus that infects sheep and causes a multi-systemic disease which can lead to death. The antigenic relatedness between HIV-1 and OPPV was examined by immunoblot analysis. Fourteen out of 20 sheep sera that were positive for OPPV on an immunodiffusion test reacted with
Specifice inhibition animal lentiviruses in cells by phosphorothioate oligodeoxynucleotides.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:339 (abstract no. T-33). Unique Identifier : AIDSLINE ASM91/0380292; Archambault D; Cohen J; Dahlberg J; Laval Univ., Ste-Foy, Quaebec, Canada.; One approach to prevent lentivirus infection, or at least limit virus spread within an infected host, is to make the use of anti-viral drugs. In this study, several phosphorothioate oligodeoxynucleotides were tested for their inhibitory effect on lentivirus replication in vitro. A 28-mer homooligomer of deoxycytidine
Retroviral sequences within the E-beta gene of the mouse MHC.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:339 (abstract no. T-35). Unique Identifier : AIDSLINE ASM91/0390292; Zimmerer EJ; Passmore HC; Murray State University, Murray, KY.; Sequence analysis in several inbred strains of mice through the second intron of the E-beta gene (MHC) revealed a major genetic rearrangement in the 5 half of this intron that separates the p and p-like haplotypes from the majority of common MHC haplotypes (b,d,k and s). A 597 bp nucleotide sequence found in the p ha
Higher frequency of HTLV-II over HTLV-I infection among Italian drug abusers.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:340 (abstract no. T-36). Unique Identifier : AIDSLINE ASM91/0400292; Ferrante P; Zella D; Mori L; Sala M; Casoli C; Magnani G; Lori F; Achilli G; Cattaneo E; Bertazzoni U; Ist. Med. Micr. Univ. Milano, Italy.; A cohort of 477 IVDAs was screened for the presence of HTLV-1 and HTLV-II with a particle agglutination assay, a WB procedure and by IFA. PBMC from 17 of 35 positive cases were further analysed by PCR amplification of tax sequences and detection with specific probes. Only one case out of 17 was confirmed positive for
Characterization of human herpes virus 6 gene segments that transactivate HIV promoter.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:341 (abstract no. T-45). Unique Identifier : AIDSLINE ASM91/0410292; Geng Y; Balachandran N; Wood C; University of Kansas, Lawrence, Kansas.; Human herpes virus 6 (HHV-6) is a recently isolated lymphotrophic herpes virus. HHV-6 can productively co-infect HIV-infected CD4+ T cells. It has been demonstrated that co-infection by both viruses can lead to accelerated cytopathic effects when compared to HIV or HHV-6 infection alone. We have demonstrated earlier t
Molecular cloning and characterization of a nuclear factor involved in the restricted HIV-1 expression in monocyte THP-1 cells.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:341 (abstract no. T-46). Unique Identifier : AIDSLINE ASM91/0420292; Mikovits J; Calvert I; West M; Young H; Ruscetti F; Kung H; Raziuddin; LBP, BRMP, BCDP, DCT, and PRI/DynCorp, National Cancer Institute,; FCRDC, Frederick, MD 21702-1201.; Monocyte/macrophage serves as a reservoir for HIV infection and plays a significant role in the pathogenesis of AIDS. We have established an HIV-1 infected human monocytic cell line THP-1 which showed multiple levels of viral expression; namely, 1) restricted or chronic low level expression and 2) high level expressio
L3T4+ subset of T-lymphocytes are responsible for paralysis and immunodeficiency induced by tsl, a mutant of moloney murine leukemia virus.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:341 (abstract no. T-47). Unique Identifier : AIDSLINE ASM91/0430292; Saha K; Wong PK; The University of Texas M. D. Anderson Cancer Center, Science; Park-Research Division, Smithville, TX 78957.; FVB mice develop hindlimb paralysis and immunodeficiency leading to death within 6 weeks when injected neonatally with a temperature-sensitive mutant (tsl) of MoMuLV. T-lymphocytes have been shown to be the primary target for tsl. In this study, we examined the role of L3T4+ (helper) T-cells in the disease pathogenesi
Quantitation of pneumocystis carinil cysts, trophozoites, and matrix in induced sputum using fluorescent monoclonal antibodies.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:349 (abstract no. C-41). Unique Identifier : AIDSLINE ASM91/0440292; Hulme P; Raviglione M; Currier J; Hanff P; Beth Israel Hospital, Harvard Medical School, Boston, MA.; Recent data suggest that the detection of Pneumocystis carinil (Pc) is more difficult in patients on aerosolized pentamidine (AP) prophyiaxis. To examine this issue, we quantitated the number of Pc cysts, trrophozoites, and matrix on positive slides made May 1989-November 1990 using a direct immunofluorescent monoclon
Evaluation of three stains for detection of Pneumocystis carinii.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:349 (abstract no. C-42). Unique Identifier : AIDSLINE ASM91/0450292; De Girolami PC; Dakos J; Karafotias G; Eichelberger K; New England Deaconess Hospital, Boston, MA.; We compared two direct immunofluorescent monoclonal antibody tests (DFA) from Genetic Systems (GS) and Meridian Diagnostics (MD) with a conventional Gram Weigert (GW) stain for rapid detection of Pneumocystis carinii (PC) in respiratory specimens. We tested 62 specimens including 26 bronchoalveolar lavage (BAL), 34 in
Diagnosis of disseminated human cytomegalovirus (HCMV) infection in patients with AIDS.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:353 (abstract no. C-66). Unique Identifier : AIDSLINE ASM91/0460292; Gerna G; Revello MG; Zipeto D; Parea M; Percivalle E; Univ. of Pavia and CNR Inst. of Genetics, Pavia, Italy.; Forty-seven AIDS patients were investigated for HCMV viremia when potentially HCMV-related symptoms were observed. Polymorphonuclear leukocytes (PMNL) from 153 blood samples were examined for: i) early virus isolation 24 h p.i. (viremia); ii) immediate-early antigen detection in PMNL (antigenemia); iii) HCMV DNA in bl
Evaluation of samples positive for cytomegalovirus (CMV) by polymerase chain reaction (PCR) for potenial confounding effect of HIV coinfection.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:353 (abstract no. C-67). Unique Identifier : AIDSLINE ASM91/0470292; Rockis M; Ferreira-Centeno J; Comerford J; Porter-Jordan K; Rodriguez R; Keiser J; Caparas MM; Ross A; Garrett C; George Washington University, Washington, DC.; PCR is considerably more sensitive than tissue culture (TC) for detection of CMV (Porter- Jordan et al., J Med Virol 30:85, 90) but the clinical significance of a positive resu
Evaluation of a rapid HIV-1 antigen monoclonal enzyme immunoassay.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:354 (abstract no. C-74). Unique Identifier : AIDSLINE ASM91/0480292; Rabito F; Pankey G; Mackenzie B; Cooper E; Leete J; Ochsner Clinic and Alton Ochsner Medical Foundation, New Orleans,; Louisiana.; Sera from 26 AIDS, 24 ARC, and 41 asymptomatic HIV-1 ELISA and Western Blot antibody-positive patients were tested, comparing a standard overnight HIV-1 antigen enzyme immunoassay (Abbott) with a rapid sandwich solid phase monoclonal enzyme immunoassay (Abbott) completed in 3.5 hours using an Abbott Dynamic Incubator.
Comparison of polymerase chain reaction (PCR) and virus culture for the detection of HIV-1 in pediatric patients.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:354 (abstract no. C-75). Unique Identifier : AIDSLINE ASM91/0490292; Hodinka RL; Titus DL; Dubin G; Friedman HM; Rutstein RM; Children's Hospital of Philadelphia and Univ. of Pennsylvania,; Philadelphia, PA.; PCR was compared with virus culture for the detection of HIV-1 from pediatric specimens. The study population included children infected with HIV, those born to HIV-seropositive mothers and children at low risk for HIV infection. A total of 67 specimens from 51 patients were examined. Peripheral blood mononuclear cell
Effect of the AIDS epidemic on the practice of clinical microbiology at a large acute care hospital.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:355 (abstract no. C-82). Unique Identifier : AIDSLINE ASM91/0500292; Southern PM; Byrd LA; Gander RM; The University of Texas Southwestern Medical Center and Parkland; Memorial Hospital, Dallas, Texas.; Parkland Memorial Hospital is a 900 bed county hospital that serves as a major teaching institution for our medical center. From 1980-90, bed size did not change appreciably, although major renovations occurred. Hospital admissions have increased by 130% since 1980, outpatient visits (ER and clinics) by 125%. An AIDS
In-Vitro susceptibility of dapsone against M. tuberculosis (MTB), and strains of M. avium (MA), and M. intracellulare (MI) isolated from AIDS patients.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:366 (abstract no. C-146). Unique Identifier : AIDSLINE ASM91/0510292; Kerkering TM; Espinel-Ingroff A; Dalton H; Warren N; Medical College of Virginia/VCU and State Consolidated; Laboratories, Richmond, VA.; The in-vitro susceptibility of dapsone against three genera of Mycobacteria was determined using a standardized disc elution agar test employing Mycobacteria 7H11 agar. MTB were from non-AIDS, and MA and MI were from AIDS pts. MA was differentiated from MI by DNA probe. 0.1ml. volumes of a 10(-3) and 10(-5) dilution o
Peritoneal dialysis related peritonitis in HIV positive vs. HIV negative patients.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:394 (abstract no. C-315). Unique Identifier : AIDSLINE ASM91/0520292; Marton R; Spinelli L; Slim J; Johnson E; Saint Michael's Medical Center, Newark, New Jersey.; The only therapeutic modalities available for HIV infected patients with end kidney disease (ESKD) are chronic ambulatory peritoneal dialysis (CAPD) or Hemodialysis (HD). A 6 year retrospective chart review was conducted in an inner city hospital of 91 patients undergoing CAPD to compare the incidence of peritonitis i
Treatment of human serum with a detergent/solvent combination for inactivation of human immunodeficiency virus (HIV) and hepatitis b virus (HBV) prior to determination of antibody levels to Neisseria meningitidis group A polysaccharide by ELISA.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:396 (abstract no. C-327). Unique Identifier : AIDSLINE ASM91/0530292; Bibb WF; Dewitt WE; Helsel LO; Carlone GM; Gheesling LL; Turner SH; Swaminathan B; Broome CV; CDC, Atlanta, GA. 30333.; The potential for occupational transmission of HIV and HBV may exist during manipulation of sera in the laboratory. A combination of 0.2% sodium cholate and 0.3% tri(n-butyl) phosphate has been shown to inactivate these viruses. Using a standardized ELISA, we studied the effects of these reagents on quantitation of an
Standardisation of an enzyme immunoassay for detection of IgG and IgM antibody against Hemophilus Ducreyi: time, HIV infection and performance.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:397 (abstract no. C-330). Unique Identifier : AIDSLINE ASM91/0540292; Desjardins M; Filion LG; Garber GE; Thompson CE; Cameron DW; Department of Microbiology and Immunology, University of Ottawa,; Ottawa, Ontario.; We standardised an enzyme immunoassay (EIA) for H. ducreyi IgG and IgM class antibodies to soluble antigen after serum absorption. A panel of 500 culture positive chancroid case and STD-negative control sera having substantial HIV prevalence was used for constructing receiver operator characteristic curves. We compare
Evaluation of the Cambridge Bioscience Recombigen HIV-1 EIA as a supplementary test for detection of antibodies to human immunodeficiency virus type 1.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:398 (abstract no. C-335). Unique Identifier : AIDSLINE ASM91/0550292; Starkey C; Yen-Lieberman B; Proffitt MR; The Cleveland Clinic Foundation, Cleveland, OH 44195.; We compared the performance of the Cambridge Bioscience (CB) Recombigen HIV-1 EIA, with a viral lysate-based screening assay, Genetic Systems (GS) LAV EIA, for the detection of antibodies to HIV-1. Of 473 sera tested, 169 were from kidney dialysis patients and 304 were from other sources, including STD and drug clini
Comparison of a recombinant antigen enzyme-immunoassay (EIA) with a viral lysate-based reference procedure for human immunodeficiency virus type 1 (HIV-1) serology.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:398 (abstract no. C-339). Unique Identifier : AIDSLINE ASM91/0560292; Wilcoski LM; Senf KL; Stevens JM; Janda WM; Univ. of Illinois Hosp., Chicago, IL.; Most laboratories performing HIV-1 serology use a viral lysated-based EIA and the Western blot supplemental test. Recombinant antigen-based EIAs have also been approved for screening serum specimens. In this study, the MicroTRAK HIV-1 recombinant antigen EIA (R-EIA, SYVA) was compared with a viral lysate-based EIA (L-
Hepatitis b delta HIV-1 HTLV-1 serologic profile in patients with malignancy in Gizan area of Saudi Arabia: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:400 (abstract no. C-347). Unique Identifier : AIDSLINE ASM91/0570292; Arya SC; Ashraf SJ; Pathak VP; Nanda V; King Fahad Centrai Hospital, Gizan, Saudi Arabia.; Serologic markers for HBV, HDV, HIV-1, HTLV-1 were tested in 42 patients with primary hepatocellular carcinoma (PHC). 19 with epithelial malignancy (EM) and 16 with non-epithelial malignancy (NEM). Total HBV exposure was 92% in 77 cases with a PHC, EM and NEM, with 75% HBsAg positives, 3.8% anti-HBc positive and 12.9%
Evaluation of the efficacy of subculture and extended incubation of BACTEC NR 660 blood culture media to detect fungemia.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:400 (abstract no. C-349). Unique Identifier : AIDSLINE ASM91/0580292; Robinson A; Binns L; Rhode Island Hospital and Brown University, Providence, RI.; The number of requests for fungal blood cultures has increased in our laboratory during the past 4 years. To assess the cost effectiveness of the more labor intensive fungal blood culture method, the respective values of 5 versus 28 days of incubation and blind subculture of BACTEC NR-660 culture media were assessed.
Unnamed gram negative rod isolated from AIDS patient description and changes on subculture.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:404 (abstract no. C-372). Unique Identifier : AIDSLINE ASM91/0590292; Clarridge JE; Simon BB; McGee H; VA Med. Ctr. and Baylor Col. of Med., Houston, TX.; The isolation of an unusual, unnamed GNR from HIV+ patients has been reported in the last year. We describe a similar, but not identical organism isolated from the blood of an AIDS patient on three occasions over a four month period. The cultures, positive only by lysiscentrifugation, produced small slow growing, rais
The seroprevalence of human immunodeficiency virus, hepatitis B virus, hepatitis C virus, and human T-lymphotropic virus type I in a population of intravenous drug users in Albuquerque, New Mexico.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:404 (abstract no. C-376). Unique Identifier : AIDSLINE ASM91/0600292; Oty G; Levy A; Steece R; Voorhees R; Scientific Lab. Div., Albuquerque, NM.; From January of 1989 through September of 1990, 188 intravenous drug users admitted to a drug treatment clinic were tested for antibody to human immunodeficiency virus (anti-HIV) as part of a blinded nationwide seroprevalence study funded by the Centers for Disease Control. Additional tests to determine the serostatus
The incidence of HIV antibodies in persons with anti-treponemal antibodies in a New York City Hospital.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:405 (abstract no. C-377). Unique Identifier : AIDSLINE ASM91/0610292; Jones A; Lue YA; Grossman WS; Hewlett D Jr; Bolivar J; Lincoln Medical and New York Medical College, Bronx, New York,; NY.; There have been several reports which have associated the increase incidence of syphilis with illicit drug use. HIV infection has been associated with intravenous drug use. To determine the incidence of HIV antibody in individuals with specific treponemal antibodies, a random sampling of specimens positive and negativ
Prevalence of hepatitis B surface antigen, HIV-1 antibody and hepatitis C antibody among admissions to a Canadian hospital.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:405 (abstract no. C-378). Unique Identifier : AIDSLINE ASM91/0620292; Louie M; Low D; Feinman S; Chua R; Mitchell B; Baillargeon R; Simor A; Mount Sinai Hospital, Univ. of Toronto, Toronto, Canada.; The prevalence of hepatitis B surface antigen (HBsAG) and antibody to HIV (anti-HIV) and hepatitis C (anti-HCV) was determined in serial unselected adult admissions to a 450-bed tertiary care hospital. An anonymous unlinked serosurvey was done using sera collected from emergency and elective admissions for 6 months in
Clinical findings associated with isolation of Chlamydia sp. from BAL specimens.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:405 (abstract no. C-379). Unique Identifier : AIDSLINE ASM91/0630292; Hall C; Bugg F; Baselski V; University of Tennessee, Dept of Pathology, Memphis, TN.; With the recognition of Chlamydia pneumoniae as an important etiologic agent of pneumonia, in 1987 we instituted routine testing of bronchoalveolar lavage specimens (BAL) for Chlamydia sp. by McCoy cell culture and direct fluorescent antibody staining (DFA) using genus specific monoclonal reagents. A total of 8 cultur
Prevalence of neurosyphilis in HIV-infected patients with positive RPR.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:405 (abstract no. C-380). Unique Identifier : AIDSLINE ASM91/0640292; Holtom PD; Larsen RA; Leal ME; University of Southern California, Los Angeles, CA.; OBJECTIVE: To determine the prevalence of CSF VDRL-positive neurosyphilis in HIV-infected patients with positive serum RPR and MHA-TP. METHODS: HIV-infected patients initially seen at the LAC+USC Medical Center AIDS clinic from June to Dec 1990 with a positive serum RPR and no recent syphilis therapy were offered an L
Shigella bacteremia in AIDS patients.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:405 (abstract no. C-381). Unique Identifier : AIDSLINE ASM91/0650292; Kathpalia S; Hubbard-Shephard M; Dorigan F; Kocka FE; Cook County Hospital-Hektoen Institute, Chicago, IL 60612.; Shigella bacteremia in the general population is rare, however, it has been occasionally reported in AIDS patients. During the past 3 years we have encountered 3 cases of Shigella flexneri bacteremia in patients with AIDS. All 3 patients were homosexual, 2 were known to have gay bowel disease with S. flexneri prior to
Etiology of diarrhea among HIV-infected infants in Zaire.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:406 (abstract no. C-384). Unique Identifier : AIDSLINE ASM91/0660292; Wells J; Puhr N; Pavia A; Bopp C; Long E; Ryder R; Nsa W; Martin P; Griffin P; Centers for Disease Control, Atlanta, GA.; During March and April 1988, stool swabs were collected from 83 infants (ages 10-15 months) with diarrhea and 58 without diarrhea in Kinshasa, Zaire . Nine ill infants and 1 well infant were infected with HIV-1. Specimens were examined for Salmonella, Shigella, Campylobacter, Yersi
Treatment of non-meningeal cryptococcal disease in HIV-infected persons.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:409 (abstract no. F-4). Unique Identifier : AIDSLINE ASM91/0670292; Jones B; Larsen RA; Forthal D; Haghighat D; Bozzette S; California Collaborative Treatment Group USC, UCI and UCSD, CA.; OBJECTIVE: To evaluate the safety and efficacy of fluconazole and flucytosine in preventing evolution of cryptococcal meningitis in HIV positive persons with non-meningeal cryptococcosis. METHODS: Patients with non-meningeal cryptococcal infection were treated with fluconazole (400 mg/d) combined with flucytosine (150
Genetic variation among members of cryptococcus neoformans var. neoformans detected by cDNA and genomic probes.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:421 (abstract no. F-80). Unique Identifier : AIDSLINE ASM91/0680292; Spitzer ED; Spitzer SG; Dept. of Pathology, SUNY at Stony Brook, Stony Brook, NY.; C. neoformans is a leading cause of life-threatening infections in patients with AIDS. The ability to identify and analyze genetic differences among clinical isolates of C. neoformans would contribute to a better understanding of the virulence properties of this environmentally acquired pathogen. We have isolated seve
Evaluation of commercially available human immunodeficiency Virus-1 antibody immunoblotting kits.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:432 (abstract no. V-7). Unique Identifier : AIDSLINE ASM91/0690292; Warner NA; Leland DS; Suter LS; Indiana University Medical Center, Indianapolis, IN 46202.; Immunoblot (IB) kits for confirming the identity of human immunodeficiency virus-1 (HIV-1) antibodies are now available commercially. The purpose of this study was to compare IB kits from Bio-Rad, Ortho Diagnostics (formerly marketed by DuPont), American Bionetics (ABN), and Organon Teknika. A panel of 27 sera, 19 of
An enzyme immunoassay (EIA) to detect antibodies to human T-lymphotropic virus type-I in human plasma and serum.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:432 (abstract no. V-8). Unique Identifier : AIDSLINE ASM91/0700292; Anton-Byrnes J; Cole C; Smith L; Nath N; Nicol E; Baxter Diagnostics, Mundelein, IL.; Human T-Lymphotropic Virus Type-I (HTLV-I) has been associated with adult T-cell leukemia/lymphoma (ATL), tropical spastic paraparesis (TSP) and HTLV-I-associated myelopathy (HAM). An EIA has been developed for screening blood to prevent transmission of HTLV-I in cellular components during transfusion. The assay utili
An unique role of microbiologists in meeting the challenge of AIDS education.: Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9;91:437 (abstract no. ED-13). Unique Identifier : AIDSLINE ASM91/0710292; Manuselis G; The Ohio State University, Columbus, OH.; With over 125,000 current cases, the aquired immunodeficiency syndrome (AIDS) continues to tax our traditional health care system. Due to increased numbers and increased longevity of AIDS patients, more health professionals will be exposed to and involved in the treatment of these individuals in the future. As the epi
Pathogenic (SIVmac-239) and nonpathogenic (SIVmac-1A11) molecular clones of SIV have distinct tissue distributions that vary with length of infection.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:21 (abstract no. 1). Unique Identifier : AIDSLINE PRIM9/0720292; Lackner AA; Vogel P; Hoogenboom E; Dandekar S; Kluge JD; Marthas M; New Mexico Regional Primate Research Laboratory, NMSU and; California Regional Primate Research Center, UC Davis.; To examine the pathogenesis of SIV we inoculated two groups of eight rhesus macaques with equal doses of either a pathogenic or nonpathogenic molecular clone of SIVmac (SIVmac-239 and SIVmac-1A11 respectively) and compared them to a third group of eight rhesus inoculated with an uncloned, biologic isolate of SIVmac (d
Development of a macrophage-topic, neurovirulent strain of SIV by multiple intracerebral passage.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:22 (abstract no. 2). Unique Identifier : AIDSLINE PRIM9/0730292; Zink MC; Sharma D; Adams RJ; Narayan O; Johns Hopkins University School of Medicine, Div. Comparative; Medicine, Baltimore MD 21205.; In man, HIV causes both non-suppurative encephalitis, and opportunistic infections secondary to immunosuppression. Whereas the immunosuppression is a result of the systemic depletion of infected CD4+ cells, studies using double staining on the brains of AIDS patients with dementia
Correlates of SIV-induced lesions in the central nervous system of experimentally infected rhesus monkeys.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:23 (abstract no. 3). Unique Identifier : AIDSLINE PRIM9/0740292; Baskin GB; Murphey-Corb M; Roberts ED; Didier PJ; Tulane Regional Primate Research Center, Tulane University,; Covington, Louisiana.; Necropsy records were reviewed from 204 SIV-infected rhesus monkeys that had been inoculated with various strains of SIV (166/204 with SIVDeltaB670) and had died of SIV-related disease. Of these, 66/204 (32%) had SIV-induced lesions in the brain parenchyma (range 0 - 62%/study); 24/204 (12%) had SIV-induced lesions in
Pathogenisis of the genital transmission of SIV: localization of SIV in the reproductive tract of chronically infected rhesus macaques.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:24 (abstract no. 4). Unique Identifier : AIDSLINE PRIM9/0750292; Miller CI; Vogel P; Hendrickx A; Alexander N; Dandekar S; Marx P; California Regional Primate Research Center, University of; California, Davis; An animal model for the heterosexual transmission of HIV was developed by infusing cell-free SIV into the vagina of female rhesus macaques or onto the foreskin glans and urethral os of male rhesus macaques. Animals inoculated by the genital route become persistently viremic and eventually develop simian AIDS. Thus the
Early pathogenesis of infection by SIVsmmPBj14 molecular clone 1.9 in macaques.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:25 (abstract no. 5). Unique Identifier : AIDSLINE PRIM9/0760292; Hoover EA; Israel ZR; Maul DH; Dean GA; O'Neil SP; Dreitz MJ; Mullins JI; Fultz PN; Department of Pathology, Colorado State University, Fort Collins,; CO 80523, USA.; We have studied the early pathogenesis of infection by molecular clone 1.9 of simian immunodeficiency virus (SIV) sooty mangabey (SMM) strain PBj14 in 4 pig-tailed (Macacca nemestrina) and 2 cynomolgus macaques (Macacca fasicularis) infected intravenously with either 6.3 x 10(4) (2 animals) or 6.3 x 10(3) TCID50 (4 an
Molecular cloning of SIVsmm/PBJ and identification of pathogenic determinants.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:26 (abstract no. 6). Unique Identifier : AIDSLINE PRIM9/0770292; Novembre FI; Hirsch VM; McClure HM; Johnson PR; Department of Microbiology, Georgetown University, and Laboratory; of Infectious Diseases, NIAID/NIH, Rockville, MD.; SIVsmm/PBj (PBj) induces a rapidly fatal disease (acute death syndrome) when inoculated into macaques. This syndrome, in contrast to the protracted immunodeficiency ordinarily associated with SIV, is characterized by a fulminant diarrhea and acidosis leading to death in 5 to 14 days. Genetic analysis of molecularly cl
Genetic mapping of viral determinants of SIV pathogenesis.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:27 (abstract no. 7). Unique Identifier : AIDSLINE PRIM9/0780292; Marthas M; Lohman B; Van Rompay K; Banapour B; Ramos R; Unger R; Higgins J; Torten J; Luciw P; Pedersen N; California Regional Primate Research Center, University of; California, Davis, CA 95616.; To identify viral determinants of pathogenesis, we constructed pairs of reciprocal recombinants between two molecular clones of SIVMac which are pathogenic (239) and a non-pathogenic (1A11). A large (6.2 kb) fragment containing gag, pol and env as well as regulatory genes (vpr, vif, and the first exons of tat and rev)
In vivo macrophage tropism of pathogenic and non-pathogenic molecular clones and recombinants of simian immunodeficiency virus (SIVmac).: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:28 (abstract no. 8). Unique Identifier : AIDSLINE PRIM9/0790292; Unger RE; Marthas ML; Lackner AA; Pratt-Lowe E; Lohman BL; Ronpay KV; Luciw PA; Department of Medical Pathology and the California Regional; Primate Research Center, University of California, Davis, 95616.; In vitro macrophage tropism differs between different molecular clones of SIVmac and recombinants between these clones. SIVmac1A11 is highly macrophage tropic for rhesus monocyte-derived and alveolar macrophages and SIVmac239 does not infect macrophages under these in vitro conditions (Banapour et al. Virology 183:12-
Modulation of SIV replication in macrophage cultures by neutralizing antibodies from SIV infected macaques.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:29 (abstract no. 9). Unique Identifier : AIDSLINE PRIM9/0800292; McEntee M; Anderson M; Zink MC; Clements J; Narayan O; Johns Hopkins University School of Medicine, Baltimore, MD.; The lentiviruses HIV and SIV cause productive infections in CD4+ lymphocytes and macrophages. The life-cycle of the virus is approximately 24 hours but inoculated cells require several days to reach their full productive potential. In vivo, infected cells rarely reach this potential during the subclinical phase of inf
Arteriopathy in macaques infected with simian immunodeficiency virus (SIVmac).: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:30 (abstract no. 10). Unique Identifier : AIDSLINE PRIM9/0810292; Chalifoux LV; Wyand MS; Desrosiers RC; Ringler DJ; Harvard Medical School, New England Regional Primate Research; Center, 1 Pine Hill Dr., Southborough, MA, USA.; Arteriopathy has been associated with human pediatric AIDS cases. A similar arteriosclerosis was seen in 19 of 85 Macaca mulatta infected with SIVmac. Fourteen were experimentally inoculated with uncloned SIVmac251, two were inoculated with tissue or blood from other SIVmac251-infected monkeys, two were naturally infe
Receptor-mediated activation of SIVagm by sCD4.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:31 (abstract no. 11). Unique Identifier : AIDSLINE PRIM9/0820292; Allan JS; Whitehead E; Hart T; Buck D; Bugelski P; Southwest Foundation for Biomedical Research, San Antonio, Texas.; The process of viral entry requires a complex series of conformational changes known as activation steps for the induction of viral fusion. As an example, influenza viruses undergo pH induced conformational changes which are thought to facilitate fusion. We previously theorized that the viral receptor for immunodefici
Gamma/delta T cells and SIV infection.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:32 (abstract no. 12). Unique Identifier : AIDSLINE PRIM9/0830292; Malkovsky M; MacKenzie D; Radtke B; Manning J; Fisch P; WRPRC, University of Wisconsin, Madison, Wisconsin.; The TCR delta 1 monoclonal antibody which reacts with all human gamma delta T cells also shows strong binding to a subset of CD3+ rhesus monkey peripheral blood mononuclear cells (4.9+/-3.0%), lymph node (4.4+/-1.6%) and speen (9.0+/-2.5%) cells (the values in parentheses represent means+/-SDs). The monkey TCR delta 1
Preferential replication of a clinical HIV-1 isolate in chimpanzee peripheral blood mononuclear cells.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:33 (abstract no. 13). Unique Identifier : AIDSLINE PRIM9/0840292; Fultz PN; Keddie E; Ghosh S; Shaw G; University of Alabama at Birmingham, Birmingham, AL 35294.; Previously, a chimpanzee-passaged strain of HIV-1, termed LAV-1b, was shown to replicate in chimpanzee PBMC and macrophages more efficiently than all other isolates of HIV-1 tested. LAV-1b also was shown to be cytopathic for both chimpanzee and human CD4+ lymphocytes, but the virus replicated equally well or better in
Simian virus-40 (SV40)-induced disease in macaques with AIDS.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:34 (abstract no. 14). Unique Identifier : AIDSLINE PRIM9/0850292; Horvath CJ; Simon MA; Bergsagel DJ; Garcea RL; Ringler DJ; Harvard Medical School, NERPRC, Southborough, MA, 01772-9102.; Recrudescence of latent DNA virus infections (e.g. herpes simplex, varicella-zoster) is a prominent feature of human AIDS. The Polyomavirinae subfamily of Papovaviridae includes DNA viruses which remain latent in renal tissue of humans (JCV and BKV) and rhesus monkeys (SV40). Polyomaviral disease in the natural host i
The role of genetic change in tissue tropism of SIVmac during the development of AIDS.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:35 (abstract no. 15). Unique Identifier : AIDSLINE PRIM9/0860292; Kodama T; Mori K; Ringler DJ; Desrosiers RC; New England Regional Primate Research Center, Harvard Medical; School, Southborough, MA USA.; Molecularly cloned SIVmac239 induces AIDS and death in the common rhesus monkey. As with HIV-induced disease in humans, the disease induced by SIVmac239 is heterogenous in nature. Rhesus monkey 316-85 died 5 1/2 months following infection with cloned SIVmac239 with generalized lymphoid depletion, giant cell pneumonia
Cell-specific variation of SIV/MNE in vitro and in vivo.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:36 (abstract no. 16). Unique Identifier : AIDSLINE PRIM9/0870292; Rudensey L; Wilson J; Papenhausen M; Overbaugh J; Department of Microbiology and Regional Primate Research Center,; University of Washington, Seattle, WA.; We have previosly reported the identification of two highly variable domains in the SIV/Mne env gene that correspond to V1 and V4 in HIV env. Other regions known to be variable in HIV, such as the V3 loop, were well conserved following SIV infection in vivo. In V1, change was detectable before CD4 depletion, but becam
Synthetic peptides designed to inhibit the HIV protease inhibit the protease of mason pfizer monkey virus preventing maturation and cleavage of gp22(TM) to gp20.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:37 (abstract no. 18). Unique Identifier : AIDSLINE PRIM9/0880292; Sommerfelt MA; Hunter E; University of Alabama at Birmingham, Dept. of Microbiology,; Birmingham, AL. 35294.; The MPMV protease is defined as an aspartyl protease and is responsible for cleavage of structural gene precursor proteins either during or shortly after budding in the process of maturation. The MPMV transmembrane (TM) envelope glycoprotein is initially cleaved from the Pr86 envelope precursor by a Golgi endopeptidas
Sequence analysis of SIVmac251 (32H reisolate) direct from macaques following infection.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:38 (abstract no. 17). Unique Identifier : AIDSLINE PRIM9/0890292; Almond N; Slade A; Jenkins A; Jones S; Chan WL; Flanagan B; Gaisford W; Ling C; Rose J; Page M; et al; NIBSC, Potters Bar, UK.; An important step in our understanding of virus-host interactions is the characterisation of virus sequences which are important in vivo and how these sequences may change during the course of infection and disease. Two cynomolgus macaques were infected with 10 MID50 of the 11/88 pool of the 32H reisolate of SIVmac251
The LTRs of two strains of SIVmac are differentially transactivated.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:39 (abstract no. 19). Unique Identifier : AIDSLINE PRIM9/0900292; Anderson M; Clements JE; The Johns Hopkins University School of Medicine Baltimore,; Maryland 21205 USA.; Two infectious molecular clones of the simian immunodeficiency virus - SIVmac251 and SIVmac239 have been found to have very different biologic effects in vivo as well as different transcriptional activities in cell culture. While SIVmac239 can cause AIDS-like symptons and death in 6 months or less, SIVmac251 causes a
Propagation of SIV vectors by genetic complementation.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:40 (abstract no. 20). Unique Identifier : AIDSLINE PRIM9/0910292; Rizvi TA; Panganiban A; McArdle Laboratory for Cancer Research, University of Wisconsin,; Madison, WI 53706.; In order to study SIV replication over a single round of replication we generated SIV239 virus particles that contain a replication defective vector containing a selectable marker. Genetic complementation between an env deficient SIV variant and plasmid that expresses the env gene of an amphotropic murine retrovirus r
SIV from Sykes' monkeys: prototype for a fifth group of primate lentivruses.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:41 (abstract no. 21). Unique Identifier : AIDSLINE PRIM9/0920292; Hirsch VM; Dapolito G; McClure H; Isahakaia M; Fultz PN; Johnson PR; Dept. of Microbiology, Georgetown University and Laboratory of; Infectious Diseases, NIAID, NIH, Rockville, Md.; Four distinct groups of primate lentiviruses have been identified to date: (i) HIV-1, including SIVcpz from a chimpanzee; (ii) HIV-2, a including SIV from sooty mangabeys (SIVsm), and SIV from macaques (SIVmac); (iii) SIV from African green monkeys (SIVagm); and, (iv) SIV from mandrills (SIVmnd). Sykes monkeys (Cerco
Maternal-fetal transmission of SIV in macaques.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:42 (abstract no. 22). Unique Identifier : AIDSLINE PRIM9/0930292; Ochs HD; Morton WR; Zhu Q; Kuller LD; Thouless ME; Benveniste RE; Department of Pediatrics, University of Washington, Seattle, WA.; To study the mechanisms of maternal-fetal transmission of HIV and to test strategies to prevent infection of the fetus, we have developed a model of maternal-fetal transmission in macaca (M.) nemestrina using the primate lymphotropic immunodeficiency virus, SIV/Mne. To date we have inoculated intravenously twelve M. n
Vaccination of pregnant cynomolgus monkeys with whole inactivated SIVmac.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:43 (abstract no. 23). Unique Identifier : AIDSLINE PRIM9/0940292; Titti F; Borsetti A; Geraci A; Sernicola L; Giuseppini L; Turillazzi GP; Pace M; Baroncelli S; Chieco-Bianchi L; Verani P; et al; Lab. Virology, Istituto Superiore di Sanitga Rome; The aim of the work was to study the antibody response in monkeys immunized during pregnancy, the effect, if any, of immunization on foetal growth, and passive transfer of antibodies to offsprings. Five pregnant (2-3.5 months) Macaca fascicularis were immunized with sucrose-gradient purified and formalin inactivated w
Decline in the CD4% and CD4/CD8 ratios in blood of SIV infected monkeys is not accompanied by concomitant decrease within the CD4+ pool present in lymph nodes.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:44 (abstract no. 24). Unique Identifier : AIDSLINE PRIM9/0950292; Rosenberg YJ; White BD; Papermaster SF; Zack PM; Jahrling PB; Eddy GA; Burke DS; Lewis MG; Henry M. Jackson Foundation Research Laboratory, Rockville, MD.; Although circulating CD4%, CD4+ cell counts and CD4/CD8 ratios are valuable in evaluating clinical progression to AIDS, it is not known how these values reflect the CD4+ cell pool as a whole. To examine this directly, lymphocytes from LN, spleen and PBMC from 16 SIV-PBj-14 infected macaques have been analyzed phenotyp
CD34+ progenitor cell, colony forming unit-granulocyte/macrophage (CFU-GM) and burst forming unit-erythroid (BFU-E) analysis in SIVsmm infected rhesus macaques (RM); functional bone marrow abnormalities without evidence of direct stem cell infection.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:45 (abstract no. 25). Unique Identifier : AIDSLINE PRIM9/0960292; Hillyer CD; Lackey DA; Villinger F; Winton EF; Ansari AA; McClure HM; Department of Pathology, Emory University, Atlanta, Georgia.; CFU-GM and BFU-E were cultured from iliac crest bone marrow aspirates of RM infected with SIVsmm9 (Macaca mulatta; n=15) and of SIV RM (n=8). Each culture plate contained 1x10(5) MNC/ml in 0.9% methylcellulose with 30% fetal calf serum, 5% rhesus PHA-LCM, 1 U/ml erythropoietin, 5x10(-5) M mercaptoethanol, penicillin a
Infection of Macaca nemestrina by human immunodeficiency virus (HIV-1).: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:46 (abstract no. 26). Unique Identifier : AIDSLINE PRIM9/0970292; Agy MB; Schmidt AM; Frumkin LR; Morton WR; Corey L; Katze MG; Regional Primate Research Center, University of Washington,; Seattle, WA 98195.; Objective: The current study was undertaken to determine the susceptibility of M. nemestrina to HIV-1 infection. Our preliminary in vitro and in vivo results are described here. Methods: (In vitro studies). Initially, peripheral blood mononuclear cells (PBMCs) were prepared from M. nemestrina, PHA stimulated and eithe
Altered course of primary syphilis in rhesus macaques with SIV infection.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:47 (abstract no. 27). Unique Identifier : AIDSLINE PRIM9/0980292; Marra CM; Handsfield HH; Kuller L; Morton WR; Lukehart SA; Department of Medicine and Regional Primate Research Center,; University of WA, Seattle, WA.; The natural history of early syphilis may be altered in HIV-infected persons, with more severe clinical manifestations and altered serological responses. Because the immunodeficiency caused by SIV and HIV are similar, we are studying the course of primary syphilis in 2 SIV-infected and 2 SIV-uninfected rhesus macaques
The anti-viral effect of AZT in rhesus monkeys infected with uncloned SIVmac251.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:48 (abstract no. 28). Unique Identifier : AIDSLINE PRIM9/0990292; Wyand MS; Partis RA; Mueller RA; Desrosiers RC; Tiemeier DC; TSI Mason Laboratories, Department of Experimental Medicine,; Worcester, MA.; The anti-viral effect of AZT was studied in a group of 10 male juvenile rhesus monkeys. Animals were randomized by weight into an untreated Group 1 (5 animals) and an AZT treated Group 2 (5 animals). AZT at 66.7 mg/kg was administered three times dail
Parenterally administered formalin-inactivated SIVmac protects rhesus macaques from intrarectal challenge.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:49 (abstract no. 29). Unique Identifier : AIDSLINE PRIM9/1000292; Cranage MP; Cook N; Dennis M; Rose J; Kitchin P; Baskerville A; Greenaway PJ; PHLS Centre for Applied Microbiology and Research, Porton Down,; Salisbury, SP4 OJG, UK.; Six rhesus macaques were inoculated intrarectally with the 11/88 vaccine challenge stock of SIVmac 32H isolate. Two animals were each inoculated with 3ml of virus at 10(-1), 10(-2) or 10(-3) respectively. Virus was recovered from both animals receiving 10(-1) and from one animal receiving 10(-2) virus dilutions from P
A formalin inactivated whole SIVmac vaccine formulated in RIBI adjuvant protects against homologous and heterologous SIV challenge.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:50 (abstract no. 30). Unique Identifier : AIDSLINE PRIM9/1010292; Putkonen P; Thorstensson R; Cranage M; Greenaway P; Albert J; Biberfeld G; The Departments of Immunology and Virology, The National; Bacteriological Laboratory, S-105 21 Stockholm, Sweden.; Eight cynomolgus monkeys received four intra-muscular 500 microgram doses of formalin inactivated SIVmac formulated in RIBI adjuvant on days 0, 30, 63 and 125. Four control animals received RIBI adjuvant only. No adverse reactions to vaccination were seen. Four vaccinated animals, together with two controls, were chal
Inactivated whole virus vaccine derived from a proviral DNA clone of SIV confers protection against homologous and heterologous challenge.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:51 (abstract no. 31). Unique Identifier : AIDSLINE PRIM9/1020292; Johnson PR; Montefiori DC; Chou J; Goldstein S; Hamm TE; Kitov S; Haigwood NL; Misher L; London WT; Gerin JL; et al; Department of Microbiology, Georgetown University, Rockville, MD.; The SIV model system has been an extremely useful tool for studying the pathogenesis, treatment, and prevention of primate lentivirus infections. With regard to vaccine development, other investigators have demonstrated in the past two years that inactivated whole-virus vaccines can prevent infection from a low dose (
Whole-inactivated and live, virulence-attenuated SIV vaccines protect rhesus macaques against infection by intravenous but not vaginal routes of challenge.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:52 (abstract no. 32). Unique Identifier : AIDSLINE PRIM9/1030292; Marthas ML; Sutjipto S; Miller C; Higgins J; Torten J; Unger R; Marx PA; Pedersen NC; California Regional Primate Research Center, University of; California, Davis, CA 95616.; We evaluated the ability of whole-inactivated and live-attenuated SIV vaccines to elicit immune responses which protect against intravenous (IV) or vaginal challenge. Four male and six female rhesus macaques received multiple intramuscular (IM) inoculations with UV-psoralen inactivated, uncloned SIVmac-251 in adjuvant
Vaccine protection against SIV infection in macaques.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:53 (abstract no. 33). Unique Identifier : AIDSLINE PRIM9/1040292; Mills KHG; Stott EJ; Kitchin PA; Gaisford W; Taffs F; Corcorran T; Silvera P; Rose J; Ling C; Bruck C; NIBSC, Potters Bar, Herts, UK.; It has previously been reported by us and others that immunisation with inactivated virus preparations can confer protection against homologous or heterologous SIV challenge. In the present study we have extended these findings to show that protection against low dose (10MID50) SIVmac 32H challenge can be achieved by
Development of recombinant vaccines against aids in primate models.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:54 (abstract no. 34). Unique Identifier : AIDSLINE PRIM9/1050292; Hu SL; Abrams K; Barber GN; Moran P; Zarling JM; Kuller L; Morton WR; Benveniste RE; Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle,; WA.; Recombinant viruses have been used to develop vaccines against retroviruses that cause AIDS-like diseases in primates. We previously showed that immunization with a recombinant vaccinia virus expressing the envelope glycoproteins of simian type D retrovirus (SRV) protected macaques against the homologous strain of SRV
Prior vaccination of macaques with conserved SIV envelope peptides does not stop initial infection but prevents transmission, disease progression and viral persistence.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:55 (abstract no. 35). Unique Identifier : AIDSLINE PRIM9/1060292; Eddy GA; Shafferman A; Lewis MG; Benveniste R; Lai CY; Jahrling PB; Eden-McCutchan F; Burke DS; Henry M. Jackson Foundation, Rockville, MD.; Earlier reports described rhesus macaques which had been immunized with a mixture of four peptides, from conserved, hydrophilic SIV envelope regions and challenged with 100 cell culture infectious doses of a biological clone of SIV/Mne. In this study they were tested for residual infectious virus and additional immune
Characterization of group specific antibodies in primates: studies with recombinant envelope antigens of HIV-1 in chimpanzees and baboons and of SIV in rhesus macaques.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:56 (abstract no. 36). Unique Identifier : AIDSLINE PRIM9/1070292; Haigwood NL; Misher LE; Planelles V; Gardner MB; Johnson PR; Steimer KS; Chiron Corporation, Emeryville CA.; Neutralizing antibodies have been shown to be protective against HIV-1 infection in chimpanzees. We have shown that high titer, cross-neutralizing activity against U.S., European, Carribean and African isolates was generated with native, glycosylated rgp120SF2 and low titer, more isolate-restricted neutralizing activi
Immune response of monkeys to a vaccination with vaccinia-HIV2Rod recombinants and purified proteins.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:57 (abstract no. 37). Unique Identifier : AIDSLINE PRIM9/1080292; Vogt G; LeGrand R; Kieny MP; Kaczorek M; Sonigo P; Girard M; Dormont D; SSA/DSV/DPTE/CEA, Fontenay aux Roses, France.; Objective: 1) To investigate the immune response of Rhesus monkeys vaccinated with a mixture of vaccinia-HIV2 recombinants, and boosted with purified proteins. 2) To determine the usefulness of anti-env response in the potential protection against HIV2. Methods: Our vaccine strategy involved different recombinant vacc
Human mesangial cells are resistant to productive infection by multiple strains of human immunodeficiency virus types 1 and 2.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:59 (abstract no. 38). Unique Identifier : AIDSLINE PRIM9/1090292; Alpers CE; McClure J; Hudkins KL; Bursten SL; University of Washington School of Medicine, Seattle, WA.; Human immunodeficienty virus-associated nephropathy (HIVAN) is a recognized clinical entity of unknown pathogenesis, which affects as many as 10% of patients infected with HIV in some medical centers. A role for viral infection of kidney cells in the initiation of this process at present is an intriguing but untested
Staphylococcal enterotoxins (superantigens) increase viral expression in SIV infected lymphocytes from macaca nemestrina.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:60 (abstract no. 39). Unique Identifier : AIDSLINE PRIM9/1100292; Axberg I; Firpo PP; Liang A; Clark EA; Regional Primate Research Center, University of Washington,; SJ-50, Seattle, WA 98195 USA.; Staphylococcal toxins, also called superantigens (SuperAg), are proteins secreted from Staphylococcus aureus (S. aureus) that can cause food poisoning in man and experimental animals. We have found that these SuperAg have a stimulatory effect on macaque peripheral blood lymphocytes (PBL) (J. Clin. Immunol., 11, in pre
Demonstration of SIV-specific structural viral proteins in infected AA-2 cells by immunoelectron microscopy.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:61 (abstract no. 40). Unique Identifier : AIDSLINE PRIM9/1110292; Brown CR; Lewis MG; Martin JE; Norton WN; Benveniste RE; Rippy MK; Zack PM; US Army Medical Research Institute of Infectious Diseases, Fort; Detrick, MD 21702.; AA-2 cells were infected with either SIVMne-E11S or SIVSM-PBj at an MOI of 1 and harvested at day 5 post-inoculation. Cells were pelleted, fixed with 2% paraformaldehyde/0.1% glutaraldehyde, dehydrated, and embedded in either PolyBed 812 epon or L R White resin. Several immunoelectron microscopic (IEM) post-embedment
Kinetics of lymph node infection following SIV inoculation in rhesus macaques.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:62 (abstract no. 41). Unique Identifier : AIDSLINE PRIM9/1120292; Chakrabarti L; Mairie MA; Hurtrel M; Montagnier L; Hurtrel B; Unitae d'Oncologie virale, Institut Pasteur, Paris, France.; Objective: to determine the sequence of events leading to acute infection and subsequent establishment of latency in lymph nodes of SIV infected rhesus macaques. Methods: Lymph nodes were obtained from 10 infected rhesus macaques that were sacrificed at days 7, 15, 30, 56, 65, 78, 98, and 105 following inoculation wit
Macrophages as early targets and reactivatable reservoirs of SIVsmmPBj14 in macaques.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:63 (abstract no. 42). Unique Identifier : AIDSLINE PRIM9/1130292; Israel ZR; Dean GA; Dow SW; O'Neil SP; Maul DH; Mullins JI; Fultz PN; Hoover EA; Department of Pathology, Colorado State University, Fort Collins,; CO 80523, USA.; Macrophages are a major reservoir of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) in the tissues of infected humans and macaques respectively. These studies were initiated to investigate the early target cells of SIVsmmPBj14-1.9 and were prompted by our observation that circulating monocy
Analysis of early intestinal abnormalities in SIV-infected rhesus macaques: a prospective study.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:64 (abstract no. 43). Unique Identifier : AIDSLINE PRIM9/1140292; Heise C; Vogel P; Lackner A; Halsted C; Dandekar S; Dept of Internal Medicine and California Primate Research Center,; University of California, Davis, CA 95616.; The goals of our research are to study pathogenic mechanisms of SIV-associated gastroenteropathy and the development of clinical malabsorption in rhesus macaques. A retrospective study of intestinal tissues from 15 SIV-infected rhesus macaques indicated a depression of jejunal brush border enzyme activities in all inf
Serum antibodies from macaques infected with SIV251 and SIV239 do not enhance receptor mediated endocytosis by macaque macrophages.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:65 (abstract no. 44). Unique Identifier : AIDSLINE PRIM9/1150292; Jolly PE; Hildreth J; Narayan O; Johns Hopkins University School of Medicine, Baltimore, MD.; Anitbody dependent enhancement (ADE) of infection by the human and simian immunodeficiency viruses (HIV and SIV) in human monocytic and lymphocytic cell lines has been reported by several investigators. In these studies enhancement occurred either by means of Fc receptor (FcR) or complement receptor (C R) mediated end
A whole blood assay for in vitro suppression of viral replication correlates with CD8+ lymphocyte medicated suppression assay in a comparison of 2 groups of SIV infected rhesus macaques.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:66 (abstract no. 45). Unique Identifier : AIDSLINE PRIM9/1160292; Lohman BL; Marthas ML; Pedersen NC; California Regional Primate Research Center, University of; California, Davis, CA.; CD8+ lymphocyte mediated supression of viral replication has been presented as a possible mechanism of aviremia in SIV infection. We developed a quick and inexpensive method for in vitro screening of SIV infected macaques for suppression of SIV replication. Briefly, washed whole blood from SIV-infected monkeys was inf
Hematologic changes in rhesus macaques experimentally infected with simian immunodeficiency virus (SIV).: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:67 (abstract no. 46). Unique Identifier : AIDSLINE PRIM9/1170292; Mandell CP; Jain NC; Lackner AA; Dandekar S; Department of Clinical Pathology, California Primate Research; Center and Department of Internal Medicine, University of; California, Davis, CA.; The objectives of the present study were to characterize the development of hematologic abnormalities in SIV-infected rhesus macaques and to identify cellular targets of SIV infection in bone marrow. Three groups of rhesus macaques, 8 monkeys each were inoculated with 1 of 3 SIV: group 1 was inoculated with an unclone
Long-term culture of rhesus macaque T lymphocytes and SIV-specific cytotoxic T lymphocyte (CTL) responses to autologous T cells infected with SIV.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:68 (abstract no. 47). Unique Identifier : AIDSLINE PRIM9/1180292; McGowan E; McChesney M; Virology and Immunology Unit, California Regional Primate; Research Center, University of California-Davis, Davis, CA.; In order to study aspects of the rhesus macaque CTL response to infection with SIV both in vitro and in vivo, we are developing methods to maintain and expand rhesus T lymphocytes in long-term culture. Peripheral blood mononuclear cells (PBMC) are stimulated with Concanavalin A and cultured in RPMI 1640 medium supplem
SIVsmmPBJ14 induced enteropathy in macaques.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:69 (abstract no. 48). Unique Identifier : AIDSLINE PRIM9/1190292; Mossman SP; O'Neil SP; Israel ZR; Maul DH; Dandekar S; Mullins JI; Fultz PN; Hoover EA; Department of Pathology, Colorado State University, Fort Collins,; CO 80523, USA.; Pigtail and cynomolgus macaques inoculated with SIVsmmPBj14 clone 1.9 undergo an acute infection characterised by profuse diarrhea, lymphadenopathy, fever and skin rash. The acute symptoms appear at about 7 days post infection and are resolved within 14 days, provided intravenous rehydration therapy is administered. T
Early morphological changes of thymus and lymph nodes during SIVmac251-infection of M. mulatta.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:70 (abstract no. 49). Unique Identifier : AIDSLINE PRIM9/1200292; Muuller IG; Schindler C; Krenn V; Kneitz C; Kerkau T; Schmausser B; Rethwilm A; Stahl-Hennig C; terMuelen V; Muuller-Hermelink HK; Inst. of Pathology, University of Wurzburg.; For the question of the pathogenesis of AIDS, lymph nodes and thymi of juvenile rhesus monkeys were morphologically investigated. Six juvenile monkey were investigated 1, 3, 6, 12, 24 and 30 weeks post intravenous infection with SIVmac251 and compared to three control animals and 6 animals of the terminal phase of inf
Natural killer activity in early and chronic SIV infection.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:71 (abstract no. 50). Unique Identifier : AIDSLINE PRIM9/1210292; O'Reilly KL; Hoover EA; Department of Pathology, Colorado State University, Fort Collins,; CO 80523, USA.; Natural killer (NK) cell activity of peripheral blood mononuclear cells from infected and uninfected pig-tailed (Macaca nemestrina) and rhesus (Macaca mulatta) macaques was examined using K562 and Raji cell targets in chromium release assays. As previously described (Vowels et al, AIDS Res Human Retroviruses 6:950, 19
Transmission of simiam immunodeficiency virus (SIV) in naturally infected African green monkeys (AGMs).: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:72 (abstract no. 51). Unique Identifier : AIDSLINE PRIM9/1220292; Otsyula M; Ochiago O; Suleman M; Gettie A; Isahakia M; Marx PA; Institute of Primate Research, PO Box 24481, Nairobi, Kenya.; To determine the mode of transmission of SIV in a breeding colony of AGMs (cercopithecus aethiops), 150 sera were screened for antibodies by ELISA and western blot. Thirty-six (25%) animals were seropositive and SIV was isolated from 15 of 36. Subsequently, virus positive and seronegative adults were used to form 8 ha
The accumulation of viral DNA in the peripheral blood mononuclear cells of simian immunodeficiency virus-infected rhesus macaques.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:73 (abstract no. 52). Unique Identifier : AIDSLINE PRIM9/1230292; Ramos RA; Unger R; Marthas M; Van Rompay K; Lackner A; Pedersen NC; Department of Medicine, School of Veterinary Medicine, University; of California, Davis, CA.; The simian immunodeficiency virus (SIV) causes an AIDS-like disease in rhesus macaques (Macaca mulatta). Several molecular clones of SIV are infectious, however, not all clones are capable of inducing simian AIDS (SAIDS). Experimental infection of macaques with SIVmac239 causes fatal disease in approximately half of t
Pathogenesis of HIV-2 infection in macaque species.: Symp Nonhum Primate Models AIDS. 1991 Nov 6-9;9:74 (abstract no. 53). Unique Identifier : AIDSLINE PRIM9/1240292; Morton WR; Schmidt AM; McClure J; Hu SL; Gale M; Kuller L; Regional Primate Research Center, University of Washington,; SJ-50, Seattle, WA, USA.; Human immunodeficiency virus type (HIV-2) has a high degree of homology to the simian immunodeficiency virus (SIV), which has been shown to cause AIDS in several species of nonhuman primates. We have identified two subgroups of HIV-2 that have differing degrees of cytopathogenicity to macaque peripheral blood mononucl