A NOVEL ZINC FINGER GENE, TZF, PREFERENTIALLY EXPRESSED IN AN HTLV-I-TRANSFORMED T-LYMPHOCYTE LINE, C10 (MEETING ABSTRACT)

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A NOVEL ZINC FINGER GENE, TZF, PREFERENTIALLY EXPRESSED IN AN HTLV-I-TRANSFORMED T-LYMPHOCYTE LINE, C10 (MEETING ABSTRACT)

Clin Res; 39(3):711A 1991. Unique Identifier : AIDSLINE ICDB/92679855
Mayer M; Yang YC; Moore J; Hromas R; Div. of Hematology/Oncology, Indiana Univ. Medical Center,; Indianapolis, IN

Abstract: The mechanism by which the human T-cell lymphoma/leukemia virus-I (HTLV) transforms T-lymphocytes is not well understood. It is independent of viral genomic insertion, and instead appears to rely on transactivation of undefined cellular growth genes. The targets of this transactivation are of great interest because they give insight into T-lymphoid oncogenesis. While searching for other members of a hematopoietic zinc finger gene family, we cloned a cDNA that may be one such target. Sequence analysis of this cDNA revealed a zinc finger of the CYS2-HIS2 class unrelated to any other in GenBank. Poly A+ Northern analysis of the tissue expression of this gene, designated TZF, found faint hybridization at multiple transcript sizes in HL60, Jurkat, JEG, Sk-HEP-1, KG1, MO7E, TMR 32, FHS 738 and K562 cells. These multiple transcripts may represent closely related zinc finger species. However, in the HTLV-I-transformed cell line C10 there was a novel highly expressed transcript approx 3 Kb in size. Poly A+ Northern analysis of the T-cell leukemia lines Jurkat and HSB and C10 cells with and without activation with TPA/PHA again revealed the selective C10 expression. This expression did not change with activation. Southern analysis found that the C10 TZF locus is not the same as non-HTLV-I transformed cells, implying that the gene is not disrupted by retroviral insertion. Thus, TZF may be one target of HTLV-I transactivation in C10 T-cells.

Keywords: Blotting, Northern Blotting, Southern Cell Line Cell Transformation, Viral/*GENETICS Cloning, Molecular DNA Insertion Elements DNA, Viral/GENETICS *Gene Expression Genome, Viral HTLV-I/*GENETICS/METABOLISM Nucleic Acid Hybridization T-Lymphocytes/*PHYSIOLOGY Trans-Activation (Genetics) Transcription, Genetic Zinc Fingers/*GENETICS ABSTRACT
920430
M9240934

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