IS901, a new member of a widespread class of atypical insertion sequences, is associated with pathogenicity in Mycobacterium avium.

Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

IS901, a new member of a widespread class of atypical insertion sequences, is associated with pathogenicity in Mycobacterium avium.

Mol Microbiol. 1991 Sep;5(9):2265-72. Unique Identifier : AIDSLINE GENBANK/X52372
Kunze ZM; Wall S; Appelberg R; Silva MT; Portaels F; McFadden JJ; Molecular Microbiology Group, School of Biological Sciences,; University of Surrey, Guildford, UK.

Abstract: An insertion sequence (IS901), found in pathogenic strains of Mycobacterium avium, but absent in M. avium complex isolates from patients with acquired immune deficiency syndrome (AIDS), has been isolated and sequenced. This insertion element has a nucleotide sequence of 1472 bp, with one open reading frame (ORF1), which codes for a protein of 401 amino acids. The amino acid sequence, terminal ends and target site of IS901 are similar to those of IS900, present in Mycobacterium paratuberculosis. However, the DNA sequences of these two IS elements exhibit only 60% homology, compared to a DNA homology of 98% between their respective hosts. IS901, like IS900, appears to belong to a family of related insertion elements present in actinomycetes and other bacteria. M. avium strains containing IS901 were found to be more virulent in mice than closely related strains lacking IS901. IS901 may be a useful tool for the study of the genetics of virulence in the M. avium complex and for obtaining stable integration of foreign genes into mycobacteria.

Keywords: Acquired Immunodeficiency Syndrome/*COMPLICATIONS Animal Base Sequence Cell Division Comparative Study DNA Insertion Elements/*GENETICS Human Liver/MICROBIOLOGY Mice Mice, Inbred BALB C Molecular Sequence Data Mycobacterium avium/*GENETICS/PATHOGENICITY Mycobacterium avium Complex/GENETICS Mycobacterium avium-intracellulare Infection/COMPLICATIONS Phylogeny Polymorphism, Restriction Fragment Length Sequence Homology, Nucleic Acid Spleen/MICROBIOLOGY Support, Non-U.S. Gov't Virulence/*GENETICS JOURNAL ARTICLE
920430
M9240325

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