Pristane induced gene activation. NLM AIDSLINE Important note: Information in this article was accurate in 1992. The state of the art may have changed since the publication date.

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Pristane induced gene activation.

Chem Biol Interact. 1992 Jan;81(1-2):119-30. Unique Identifier : AIDSLINE MED/92110860
Garrett LR; Jones CJ; Cuchens MA; Department of Microbiology University of Mississippi Medical; Center, Jackson 39216-4505.

Abstract: Studies were performed to examine the effects of 2,6,10,14-tetramethyl pentadecane (pristane) versus 12-O-tetradecanoylphorbol 13-acetate (TPA) on the activation of the CAT gene under the regulatory control of viral promoter/enhancer elements transfected into NIH-3T3, CV-1 and COS-7 cells. The results of these studies demonstrated that (1) pristane or TPA induced trans-activation of SV2cat, HIVcat, RSVcat and MMTVcat in cells transfected with each respective plasmid construct, (2) only pristane induced activation of pA10cat and pOSP/11 and (3) neither TPA nor pristane trans-activated pSV0cat. Furthermore, treatment with either pristane or TPA elicited changes in the morphology of each of the cell lines. Collectively these results indicate that pristane is a potent inducer of gene expression and exhibits similar characteristics as the tumor promoter, TPA.
Keywords: Animal Carcinogens/*PHARMACOLOGY Cell Line Cell Line, Transformed Chloramphenicol Acetyltransferase/*GENETICS/METABOLISM Comparative Study Gene Expression/DRUG EFFECTS Gene Expression Regulation, Enzymologic/*DRUG EFFECTS Genes, Structural, Bacterial Mice Plasmids Polyomavirus macacae Support, U.S. Gov't, P.H.S. Terpenes/*PHARMACOLOGY Tetradecanoylphorbol Acetate/*PHARMACOLOGY Trans-Activation (Genetics) Transfection 3T3 Cells JOURNAL ARTICLEKWDanimalcarcinogens/KWDpharmacologycelllinecellline,transformedchloramphenicolacetyltransferase/KWDgenetics/metabolismcomparativestudygeneexpression/drugeffectsgeneexpressionregulation,enzymologic/KWDdrugeffectsgenes,structural,bacterialmiceplasmidspolyomavirusmacacaesupport,uKWDsKWDgov't,pKWDhKWDsKWDterpenes/KWDpharmacologytetradecanoylphorbolacetate/KWDpharmacologytrans-activation(genetics)transfection3t3cellsjournalarticle
920430
M9240088

Copyright © 1992 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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