COUP-TF BINDS TO A NEGATIVE REGULATORY REGION IN THE HIV-1 LTR (MEETING ABSTRACT) NLM AIDSLINE Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

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COUP-TF BINDS TO A NEGATIVE REGULATORY REGION IN THE HIV-1 LTR (MEETING ABSTRACT)

Proc Annu Meet Am Assoc Cancer Res; 32:A1678 1991. Unique Identifier : AIDSLINE ICDB/91674479
Cooney AJ; Tsai SY; O'Malley BW; Tsai MJ; Dept. of Cell Biology, Baylor Coll. of Medicine, Houston, TX; 77030

Abstract: Previous studies have shown that a GGTCA palindrome within the HIV-1 LTR NRE binds a potential member of the steroid receptor superfamily. Mutation of this sequence leads to an increase in LTR-directed transcription compared to the wild type, consistent with this element's being a functional part of the NRE. The palindrome contains significant identity to the chicken ovalbumin upstream promoter (COUP) element to which COUP-TF, a member of the steroid receptor superfamily, binds. We demonstrate here that human COUP-TFs can bind specifically to this HIV-1 COUP-like element and that the predominant COUP-TF family member synthesized in T-cells is the 68-kD form, which is likely to be responsible for any in vivo function of this element in T cells. Finally, we have identified three HIV-1 variant strains that contain mutations in the HIV-1 COUP-like element which affect the binding affinity of COUP-TF for these variant COUP elements.
Keywords: Base Sequence Binding Sites *Gene Expression Regulation, Viral Human *HIV Long Terminal Repeat HIV-1/*GENETICS Mutation Receptors, Steroid/GENETICS T-Lymphocytes/IMMUNOLOGY Transcription Factors/GENETICS/*METABOLISM Variation (Genetics) ABSTRACTKWDbasesequencebindingsitesKWDgeneexpressionregulation,viralhumanKWDhivlongterminalrepeathiv-1/KWDgeneticsmutationreceptors,steroid/geneticst-lymphocytes/immunologytranscriptionfactors/genetics/KWDmetabolismvariation(genetics)abstract
910930
M9190731

Copyright © 1991 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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