Evaluation of enzyme-linked immunosorbent and alternative assays for detection of HIV antibodies using panels of Brazilian sera. NLM AIDSLINE Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

Click here to return to AIDSLINE main menu
DonateNow
Print this Article


Evaluation of enzyme-linked immunosorbent and alternative assays for detection of HIV antibodies using panels of Brazilian sera.

Rev Inst Med Trop Sao Paulo. 1990 Mar-Apr;32(2):96-100. Unique Identifier : AIDSLINE MED/91252717
Ivo-Dos-Santos J; Mello DL; Couto-Fernandez JC; Passos RM; Dias-Carneiro LA; Castilho EA; Galvao-Castro B; Fundacao Oswaldo Cruz, WHO Collaborating Centre on AIDS, Bahia,; Brazil.

Abstract: Sera from 472 Brazilian subjects, confirmed to be either positive or negative for HIV antibodies and comprising the total clinical spectrum of HIV infection, were utilized in the evaluation of six commercially available enzyme-linked immunosorbent assays (ELISA), as well as of four alternative assays, namely indirect immunofluorescence (IIF), passive hemagglutination (PHA), dot blot and Karpas AIDS cell test. The sensitivities ranged from 100% (Abbott and Roche ELISA) to 84.2% (PHA) and the specificities ranged from 99.3% (IIF) to 80.2% (PHA). The sensitivity and specificity of the PHA and the sensitivity of the Karpas cell test were significantly lower than those of the other tests. Although the IFF and dot blot had good sensitivities and specificities, the six ELISA were more attractive than those tests when other parameters such as ease of reading and duration of assay were considered.
Keywords: Blotting, Western Brazil Enzyme-Linked Immunosorbent Assay Evaluation Studies Fluorescent Antibody Technique Hemagglutination Tests Human HIV Antibodies/*ANALYSIS Sensitivity and Specificity Support, Non-U.S. Gov't JOURNAL ARTICLEKWDblotting,westernbrazilenzyme-linkedimmunosorbentassayevaluationstudiesfluorescentantibodytechniquehemagglutinationtestshumanhivantibodies/KWDanalysissensitivityandspecificitysupport,non-uKWDsKWDgov'tjournalarticle
910930
M9190676

Copyright © 1991 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1991. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1991. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .