ESTABLISHMENT AND CHARACTERIZATION OF HUMAN SOLID TUMOR CELL LINES OF VARIOUS HISTOGENESES FOR CHEMOSENSITIVITY EVALUATIONS (MEETING ABSTRACT)

Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

ESTABLISHMENT AND CHARACTERIZATION OF HUMAN SOLID TUMOR CELL LINES OF VARIOUS HISTOGENESES FOR CHEMOSENSITIVITY EVALUATIONS (MEETING ABSTRACT)

Proc Annu Meet Am Assoc Cancer Res; 32:A2281 1991. Unique Identifier : AIDSLINE ICDB/91675079
Schmid SM; Gordon DG; Dykes DJ; Harrison SD Jr; Mayo JG; Abbott BJ; Southern Res. Inst., Birmingham, AL 35255

Abstract: To help meet the demands of in vitro antitumor drug screening, cell lines were established from patient samples or xenograft tumors. Tissue samples were implanted in nude mice, carried for 1-2 passages, and then used in explant primary culture on plastic and extracellular matrices in several media. Growth kinetics, plating efficiency, cellular morphology, HIV, HHV2, MAP, and mycoplasma expression were measured. The lines were adapted to grow in RPMI 1640 media with 10% FBS. Tumorigenicity studies were conducted in nude mice from lines passaged over 5 times. All lines developed were tumorigenic in nude mice. The lines established to date include 1 gastric, 1 colon, 5 lung, and 4 melanoma. Stocks of cryopreserved cells were prepared for possible use in the NCI in vitro antitumor drug screen.

Keywords: Animal Drug Screening Assays, Antitumor/*METHODS Human Mice Mice, Nude Neoplasm Transplantation Transplantation, Heterologous Tumor Cells, Cultured/DRUG EFFECTS/*PATHOLOGY ABSTRACT
911030
M91A1119

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