[Detection and antigenic distribution of hepatitis B virus in liver tissue and its relation to other serological markers of viral replication] NLM AIDSLINE Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

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[Detection and antigenic distribution of hepatitis B virus in liver tissue and its relation to other serological markers of viral replication]

Enferm Infecc Microbiol Clin. 1990 Dec;8(10):610-7. Unique Identifier : AIDSLINE MED/91274424
Malave C; Fernandez de Aranguiz A; Sarria L; Ereno C; Campelo C; Gorrino MT; Sunen E; Cisterna R; Departamento de Microbiologia e Inmunologia, Facultad de; Medicina, Universidad del Pais Vasco, UPV/EHU, Lioa, Vizcaya.

Abstract: The aim of this work has been the production of specific monoclonal antibodies against HBV-antigens and their utilisation in order to study their distribution on liver tissue. The monoclonal antibodies anti-HBc and anti-HBs were obtained by the modified hybridoma technique. This study was performed on 50 patients affected by several chronic hepatopathies. For the detection of the antigens, avidin-biotin-peroxidase complex immunostaining was used. Both cytoplasmic and membranous HBsAg were detected in 15 out of 16 HBsAg+ patients; 8 of 12 HBsAg-/anti-HBc+ patients and 1 HBsAg-/antiHBc- patient. Cytoplasmic and nuclear HBcAg was observed in 12 of 16 HBsAg+ patients and 4 of 20 HBsAg- patients. Although the presence of serum HBsAg is an index of liver infection, in some HBsAg-/antiHB+ patients (20%) with undetectable levels of HBsAg, hepatic injury may be disclosed by the detection of other markers of active viral replication.
Keywords: Adolescence Adult Aged Antibodies, Monoclonal/DIAGNOSTIC USE Biological Markers Cell Membrane/IMMUNOLOGY Cell Nucleus/IMMUNOLOGY Child Child, Preschool Cytoplasm/IMMUNOLOGY English Abstract Female Hepatitis B/COMPLICATIONS/*MICROBIOLOGY Hepatitis B Antibodies/DIAGNOSTIC USE Hepatitis B Antigens/*ANALYSIS Hepatitis B Virus/IMMUNOLOGY/*ISOLATION & PURIF/PHYSIOLOGY Human HIV Infections/COMPLICATIONS Liver/*MICROBIOLOGY/ULTRASTRUCTURE Male Middle Age *Virus Replication JOURNAL ARTICLEKWDadolescenceadultagedantibodies,monoclonal/diagnosticusebiologicalmarkerscellmembrane/immunologycellnucleus/immunologychildchild,preschoolcytoplasm/immunologyenglishabstractfemalehepatitisb/complications/KWDmicrobiologyhepatitisbantibodies/diagnosticusehepatitisbantigens/KWDanalysishepatitisbvirus/immunology/KWDisolation&purif/physiologyhumanhivinfections/complicationsliver/KWDmicrobiology/ultrastructuremalemiddleageKWDvirusreplicationjournalarticle
911030
M91A1080

Copyright © 1991 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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