HIV-1 infection in HIV-1 enzyme-linked immunoassay seronegative patients in Kinshasa, Zaire.

Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

HIV-1 infection in HIV-1 enzyme-linked immunoassay seronegative patients in Kinshasa, Zaire.

Int J STD AIDS. 1990 Sep;1(5):330-4. Unique Identifier : AIDSLINE MED/91274462
Colebunders R; Francis H; Duma MM; van der Groen G; Lebughe I; Kapita B; Quinn TC; Heyward WL; Piot P; Projet SIDA, Department of Public Health, Kinshasa, Zaire.

Abstract: Serum samples of 62 African patients who had clinical manifestations of HIV-1 infection but were seronegative for HIV-1 by ELISA (Organon) were subsequently further tested by another HIV-1 ELISA test (Wellcozyme), HIV-1 IgG Western blot, HIV-1 antigen detection and HIV-2 ELISA. Patients' lymphocytes were cultured for HIV-1 and 2. Because of limited quantities of serum available all tests were not performed on all samples. Seven (26%) of 27 sera of patients meeting the WHO clinical case definition of AIDS were Western-blot-positive. In contrast, of 35 patients' sera with possible HIV related disease, only one (3%) was Western blot positive (P = 0.02) and none of 75 sera from HIV-1 ELISA (Organon) seronegative blood donors (P less than 0.01) were Western blot positive. Of 30 HIV-1 ELISA (Organon) seronegative patients tested with the HIV-1 ELISA Wellcozyme assay only one was seropositive (this patient's serum was also Western blot positive). Of 17 HIV-1 ELISA (Organon) seronegative patients tested, HIV-1 antigen was found in 1 case (6%) (this patient's serum was Western blot negative). None of the 34 patients tested by HIV-2 serology was HIV-2 seropositive. HIV-1 was isolated by culture in 3 (21%) of 14 HIV-1 ELISA seronegative patients (sera of the 3 patients were Western blot negative). In total, 12 (19%) of 62 HIV-1 ELISA (Organon) seronegative patients were found to be positive for HIV, either by Western blot HIV antigen testing or viral culture.(ABSTRACT TRUNCATED AT 250 WORDS)

Keywords: Adult AIDS Serodiagnosis/*METHODS Blotting, Western *Enzyme-Linked Immunosorbent Assay False Negative Reactions Female Human HIV Antigens/ANALYSIS HIV Seropositivity/*DIAGNOSIS *HIV-1 Male Support, U.S. Gov't, P.H.S. Zaire JOURNAL ARTICLE
911030
M91A1075

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