Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.
SEROEPIDEMIOLOGY AND COMPARATIVE ANALYSIS BETWEEN RETROVIRUSES ISOLATED FROM CAPTIVE BABOONS, HUMAN T-LYMPHOTROPIC VIRUS TYPE I (HTLV-I) AND SIMIAN T-LYMPHOTROPIC VIRUS TYPE I
Diss Abstr Int [B]; 51(7):3243 1991. Unique Identifier : AIDSLINE ICDB/91671030 Lazo A; Ohio State Univ.
Abstract:
Seroepidemiological studies effected during a five year period in a feral baboon colony housed at The Ohio State University, showed a high prevalence of antibodies cross-reacting with HTLV-I virus. Seroconversion rates of previously negative females as well as male baboons during the same period of time were elevated. These results indicate that the sexual route may be the most important mode of STLV-I infection. Cells lines from peripheral blood mononuclear cells from two HTLV-I seropositive baboons (P. cynocephalus) were established. These cell lines expressed and released a retrovirus with properties of STLV-I type viruses. The expression of retroviral particles in the newly established cell lines was readily demonstrated by the presence of cell membrane-associated antigens reacting positively with anti-HTLV-I polyclonal and monoclonal antibodies. The presence of reverse transcriptase activity in pelleted viruses, as well as in sucrose fractions with specific density of about 1.15 g/ml, was also indicative of retroviruses. In addition, the size and morphology of these viruses as determined by electron microscopy, were consistent with type C retroviruses. Comparison by Western blot analysis of the respective viral proteins between two baboon viruses, two Japanese macaque (M. fuscata) virus isolates and an HTLV-I virus isolate showed both identical and different epitopes as well as similarities and differences in the amount of expression of specific proteins. Results indicated that proviral DNA from baboons as well as Japanese macaque isolates were homologous with HTLV-I proviral sequences. Southern blot analysis of pst-1 DNA digests from both simian and human isolates also showed similarities and differences in restriction sites with the enzyme. The two restricted baboon isolates had three bands (1.2, 1.6, and 2.4 Kbp) in common with the human isolate, whereas the Japanese macaque had only two bands (1.2 and 1.6 Kbp). In addition, KIA, one of the baboon isolates, did not produce the 1.3 Kbp fragment common to all simian isolates. The extra bands observed with the baboon proviral DNA but not with the HTLV-I proviral DNA showed the baboon virus isolates had more pst-1 sites. We concluded that viruses from Papio species are more closely related to human retroviruses than those form the Macaca species. (Abstract shortened with permission of author: Full text available from University Microfilms International, Ann Arbor, MI, as Order No. AAD90-22520).
Keywords: Animal Cell Line Comparative Study DNA, Viral/GENETICS Female Human HTLV-I/*GENETICS/IMMUNOLOGY/ISOLATION & PURIF/ULTRASTRUCTURE HTLV-I Antibodies/ANALYSIS Macaca/MICROBIOLOGY Male Microscopy, Electron Papio/*MICROBIOLOGY Sequence Homology, Nucleic Acid Seroepidemiologic Methods Species Specificity STLV/*GENETICS/IMMUNOLOGY/ISOLATION & PURIF/ULTRASTRUCTURE THESIS 910530
M9150932
AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.
Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.
