Evaluation of leukocyte removal filters modelled by use of HIV-infected cells and DNA amplification. NLM AIDSLINE Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

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Evaluation of leukocyte removal filters modelled by use of HIV-infected cells and DNA amplification.

Blood. 1990 Nov 15;76(10):2159-61. Unique Identifier : AIDSLINE MED/91055121
Rawal BD; Schwadron R; Busch MP; Endow R; Vyas GN; Department of Laboratory Medicine, University of California, San; Francisco 94143.

Abstract: The concept of reducing cell-associated blood-borne viruses (BBVs) by filtration of the vector leukocytes from blood collected for transfusion has led to the development of high efficiency filters. Improved filtration technology demands newer methodology to accurately estimate the residual cells. We have developed an experimental model based on the hemocytometer counts and the polymerase chain reaction (PCR), performed on the lymphocytes derived from the units of red cell mass inoculated with marker cells (H9) persistently carrying cell-associated human immunodeficiency virus DNA (CA-HIV). We measured the efficiency of 6 units of a prototype filter using our model and found an estimated mean of less than 4 residual cells per milliliter in the filtered blood. This represents a mean 5.84 log10 reduction of normal PBMC and CA-HIV in pre- and post-filtration aliquots and exemplifies the application of our model for evaluating a new generation of blood filters. Our model illustrates that a biological tracer (ie, DNA) is a better measure of the efficacy of a leukocyte filter than the hemocytometric enumeration of pre- and post-filtration PBMC concentrates.
Keywords: Cell Separation/*INSTRUMENTATION/METHODS DNA, Viral/GENETICS Gene Amplification/GENETICS/*METHODS Human HIV-1/GENETICS/ISOLATION & PURIF Leukocytes/*CYTOLOGY/MICROBIOLOGY Models, Biological Polymerase Chain Reaction Support, U.S. Gov't, P.H.S. JOURNAL ARTICLEKWDcellseparation/KWDinstrumentation/methodsdna,viral/geneticsgeneamplification/genetics/KWDmethodshumanhiv-1/genetics/isolation&purifleukocytes/KWDcytology/microbiologymodels,biologicalpolymerasechainreactionsupport,uKWDsKWDgov't,pKWDhKWDsKWDjournalarticle
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Copyright © 1991 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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