CD4-Pseudomonas exotoxin conjugates delay but do not fully inhibit human immunodeficiency virus replication in lymphocytes in vitro. NLM AIDSLINE Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

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CD4-Pseudomonas exotoxin conjugates delay but do not fully inhibit human immunodeficiency virus replication in lymphocytes in vitro.

J Clin Invest. 1990 Nov;86(5):1684-9. Unique Identifier : AIDSLINE MED/91056174
Tsubota H; Winkler G; Meade HM; Jakubowski A; Thomas DW; Letvin NL; Harvard Medical School, New England Regional Primate Research; Center, Southborough, Massachusetts 01772.

Abstract: The CD4 molecule is a high affinity receptor for the human immunodeficiency virus (HIV) envelope glycoprotein (gp160 or gp120). This glycoprotein is expressed on the surface membrane of cells infected with HIV. It has, therefore, been suggested that a soluble form of CD4 might be used as a targeting agent to deliver toxins selectively to cells infected with HIV. We demonstrate that CD4-Pseudomonas exotoxin A (PE) conjugates inhibit the proliferation of gp160-transfected Chinese hamster ovary cells and block HIV replication in virus-infected H9 cells. However, this inhibition of HIV replication appears to be incomplete since virus replication occurs following removal of the toxin conjugates from these cultures. Moreover, CD4-PE conjugates delay but do not inhibit HIV replication in human peripheral blood lymphocytes. These studies suggest that such conjugates should be assessed only as potential adjunctive therapies in the acquired immunodeficiency syndrome.
Keywords: Amino Acid Sequence Animal *Antigens, CD4 Bacterial Toxins/*PHARMACOLOGY Cell Line Exotoxins/*PHARMACOLOGY Hamsters HIV/*PHYSIOLOGY Immunotoxins/*PHARMACOLOGY Lymphocytes/*MICROBIOLOGY Mice Molecular Sequence Data Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Time Factors Virus Replication JOURNAL ARTICLEKWDaminoacidsequenceanimalKWDantigens,cd4bacterialtoxins/KWDpharmacologycelllineexotoxins/KWDpharmacologyhamstershiv/KWDphysiologyimmunotoxins/KWDpharmacologylymphocytes/KWDmicrobiologymicemolecularsequencedatasupport,non-uKWDsKWDgov'tsupport,uKWDsKWDgov't,pKWDhKWDsKWDtimefactorsvirusreplicationjournalarticle
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Copyright © 1991 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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