Identification of individual human immunodeficiency virus type 1 gp120 amino acids important for CD4 receptor binding.

Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

Identification of individual human immunodeficiency virus type 1 gp120 amino acids important for CD4 receptor binding.

J Virol. 1990 Dec;64(12):5701-7. Unique Identifier : AIDSLINE MED/91056537
Olshevsky U; Helseth E; Furman C; Li J; Haseltine W; Sodroski J; Dana-Farber Cancer Institute, Department of Pathology, Harvard; Medical School, Boston, Massachusetts.

Abstract: The binding of the CD4 receptor by the human immunodeficiency virus type 1 gp120 exterior envelope glycoprotein is important for virus entry and cytopathic effect. To investigate the CD4-binding region of the gp120 glycoprotein, we altered gp120 amino acids, excluding cysteines, that are conserved among the primate immunodeficiency viruses utilizing the CD4 receptor. Changes in two hydrophobic regions (Thr-257 in conserved region 2 and Trp-427 in conserved region 4) and two hydrophilic regions (Asp-368 and Glu-370 in conserved region 3 and Asp-457 in conserved region 4) resulted in significant reductions in CD4 binding. For most of the mutations affecting these residues, the observed effects on CD4 binding did not apparently result from global conformational disruption of the gp120 molecule, as assessed by measurements of precursor processing, subunit association, and monoclonal antibody recognition. The two hydrophilic regions exhibit a strong propensity for beta-turn formation, are predicted to act as efficient B-cell epitopes, and are located adjacent to hypervariable, glycosylated regions. This study defines a small number of gp120 residues important for CD4 binding, some of which might constitute attractive targets for immunologic intervention.

Keywords: Amino Acid Sequence Animal *Antigens, CD4 Cell Line Comparative Study HIV Envelope Protein gp120/GENETICS/IMMUNOLOGY/*METABOLISM HIV-1/IMMUNOLOGY/*METABOLISM HIV-2/GENETICS Molecular Sequence Data Mutation Protein Binding Protein Conformation Restriction Mapping Sequence Homology, Nucleic Acid Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. SIV/GENETICS Transfection JOURNAL ARTICLE
910330
M9130551

AEGiS is a 501(c)3, not-for-profit, tax-exempt, educational corporation. AEGiS is made possible through unrestricted funding from Boehringer Ingelheim, Bridgestone/Firestone Charitable Trust, Bristol-Myers Squibb Company, Elton John AIDS Foundation, Gill Foundation, the National Library of Medicine, Quest Diagnostics, Roche and Trimeris, and donations from users like you. Always watch for outdated information. This article first appeared in 1991. This material is designed to support, not replace, the relationship that exists between you and your doctor.

AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.

Copyright ©1980, 1991. AEGiS. All materials appearing on AEGiS are protected by copyright as a collective work or compilation under U.S. copyright and other laws and are the property of AEGiS, or the party credited as the provider of the content. .