ANTISENSE-MEDIATED SPECIFIC INHIBITION OF P120 PROTEIN EXPRESSION AND CELL PROLIFERATION (MEETING ABSTRACT) NLM AIDSLINE Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

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ANTISENSE-MEDIATED SPECIFIC INHIBITION OF P120 PROTEIN EXPRESSION AND CELL PROLIFERATION (MEETING ABSTRACT)

Proc Annu Meet Am Assoc Cancer Res; 32:A1642 1991. Unique Identifier : AIDSLINE ICDB/91674438
Fonagy A; Wilson A; Busch H; Freeman JW; Dept. of Surgery, Univ. of Kentucky Medical Center, Lexington, KY; 40536

Abstract: Monoclonal antibodies, cDNAs, and the genomic DNA have been obtained for the proliferation-associated nucleolar antigen P120 (Freeman et al, Cancer Res 48:1244-51, 1988; Fonagy et al, Cancer Comm 1:243-51, 1989; and Larson et al, Cancer Comm 2:63-71, 1990). In this study, we report that transfection of a pentadecadeoxyribonucleotide (5'-AAAGCCCCCCACCAC-3') complementary to a splice junction site of mRNA for protein P120 inhibits mitogen-induced P120 protein expression and proliferation of human lymphocytes. Inhibition was concentration-dependent as measured by Western blot analysis and 3H-thymidine uptake; inhibition was not observed in cells transfected with a nonsense oligomer. P120 antisense treatment prevented S phase entry but did not block transition from G0 to G1 phase as assessed by morphological blast transformation and induction of 3H-uridine incorporation. This study suggests that P120 expression may be required for the upregulation of nucleolar function that is necessary for cell proliferation.
Keywords: Base Sequence Cell Cycle/DRUG EFFECTS Cell Division/*DRUG EFFECTS Cell Line DNA Replication/DRUG EFFECTS Human HIV Envelope Protein gp120/*GENETICS Lymphocyte Transformation/DRUG EFFECTS Molecular Sequence Data Oligonucleotides, Antisense/*PHARMACOLOGY RNA Splicing RNA, Messenger/DRUG EFFECTS/*GENETICS Thymidine/METABOLISM Transfection ABSTRACTKWDbasesequencecellcycle/drugeffectscelldivision/KWDdrugeffectscelllinednareplication/drugeffectshumanhivenvelopeproteingp120/KWDgeneticslymphocytetransformation/drugeffectsmolecularsequencedataoligonucleotides,antisense/KWDpharmacologyrnasplicingrna,messenger/drugeffects/KWDgeneticsthymidine/metabolismtransfectionabstract
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Copyright © 1991 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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