PHORBOL DIESTER STIMULATED HL-60 CELL DIFFERENTIATION IS ASSOCIATED WITH THE ACTIVATION OF SPECIFIC DNA BINDING PROTEINS (MEETING ABSTRACT)

Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

PHORBOL DIESTER STIMULATED HL-60 CELL DIFFERENTIATION IS ASSOCIATED WITH THE ACTIVATION OF SPECIFIC DNA BINDING PROTEINS (MEETING ABSTRACT)

Proc Annu Meet Am Assoc Cancer Res; 32:A1751 1991. Unique Identifier : AIDSLINE ICDB/91674552
Rogers M; Small GW; Samples L; Daniel LW; Dept. of Biochemistry, Bowman Gray Sch. of Medicine, Wake Forest; Univ., Winston-Salem, NC 27103-2796

Abstract: TPA-stimulated differentiation of HL-60 cells to macrophagelike cells is accompanied by morphological and biochemical changes, including cellular adhesion and expression of the lymphocyte cell adhesion molecule subunit cd 11b. Using cellular adhesion assays and direct immunofluorescence microscopy of cd 11b, we observed a direct correlation between the induction of these markers of macrophage differentiation and the activation of two chloramphenicol acetyl transferase (CAT) reporter plasmids. These plasmids contained sequences upstream of the reporter gene which are activated by the DNA binding proteins NF-kB (HIV-CAT plasmid) and AP-1 (3xTREtkCAT plasmid). HIV-CAT induction began at 24 hr and reached a max expression at 72 hr; 3xTREtkCAT activity started earlier (12 hr) and reached a max earlier (60 hr). TPA (10[-8]M) induced HIV-CAT expression and cellular adhesion was inhibited by the protein kinase C (PKC) inhibitors, ET-18-OCH3, staurosporine, and sphingosine, at nontoxic concentrations. Enhancer-DNA binding protein interactions were also measured in a gel retardation assay. These observations support a role for PKC-mediated gene activation by DNA-binding proteins in HL-60 cell differentiation.

Keywords: Cell Differentiation/DRUG EFFECTS DNA-Binding Proteins/*PHYSIOLOGY Gene Expression Regulation Human Leukemia, Promyelocytic, Acute/*PATHOLOGY Protein Kinase C/PHYSIOLOGY Tetradecanoylphorbol Acetate/*PHARMACOLOGY Tumor Cells, Cultured ABSTRACT
910730
M9170999

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