MUTATIONS INTRODUCED ALONG THE HTLV-1 ENVELOPE GENE RESULT IN A NONFUNCTIONAL PROTEIN: A BASIS FOR ENVELOPE CONSERVATION (MEETING ABSTRACT) NLM AIDSLINE Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

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MUTATIONS INTRODUCED ALONG THE HTLV-1 ENVELOPE GENE RESULT IN A NONFUNCTIONAL PROTEIN: A BASIS FOR ENVELOPE CONSERVATION (MEETING ABSTRACT)

Proc Annu Meet Am Assoc Cancer Res; 32:A1846 1991. Unique Identifier : AIDSLINE ICDB/91674648
Dokhelar MC; Pique C; Tursz T; Institut G. Roussy, Villejuif, France

Abstract: The envelope protein of the human T leukemia virus type I (HTLV-I) is highly conserved among the isolates sequenced so far, as opposed to what is observed for the human immunodeficiency virus envelope. By linker insertion scanning, we have produced 33 random mutations along the HTLV-I envelope gene, cloned into a eukaryotic expression vector. The resulting envelope products were analyzed by immunoprecipitation and syncytia formation after transfection into COS-1 cells. We show here that 25 out of 33 mutations result in a nonfunctional envelope product as assessed by the lack of ability to form syncytia. In the majority of these mutants, the processing of the envelope gp61 precursor into the mature gp40 and gp20 proteins was affected. We propose that conformational constraints for processing and fusion abilities tend to limit the variability of the HTLV-I envelope. In 3 mutants, processing was observed but no syncytia were formed. These mutations might affect regions important for HTLV-I envelope functions, such as the receptor binding region.
Keywords: Animal Cell Line *Genes, Structural, Viral HTLV-I/*GENETICS/PHYSIOLOGY Lysogeny Membrane Fusion *Mutagenesis, Insertional Protein Conformation Transfection Viral Envelope Proteins/*GENETICS ABSTRACTKWDanimalcelllineKWDgenes,structural,viralhtlv-i/KWDgenetics/physiologylysogenymembranefusionKWDmutagenesis,insertionalproteinconformationtransfectionviralenvelopeproteins/KWDgeneticsabstract
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M9170998

Copyright © 1991 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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