HUMAN GLIAL CELLS FROM FETAL BUT NOT ADULT HUMAN BRAIN ACTIVATE THE EXPRESSION OF HIV IN CHRONICALLY INFECTED MONOCYTES (MEETING ABSTRACT)

Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

HUMAN GLIAL CELLS FROM FETAL BUT NOT ADULT HUMAN BRAIN ACTIVATE THE EXPRESSION OF HIV IN CHRONICALLY INFECTED MONOCYTES (MEETING ABSTRACT)

Proc Annu Meet Am Assoc Cancer Res; 32:A1860 1991. Unique Identifier : AIDSLINE ICDB/91674662
Kalebic T; Helman L; Pizzo P; Lab. of Molecular Genetics, Pediatric Branch, NCI, Bethesda, MD; 20892

Abstract: The purpose of this study was to investigate whether human glial cell lines derived from fetal brain release soluble factors which induce the expression of latent HIV in chronically infected monocytes. As a cellular model for HIV latency, we used the promonocytic U1 cell line, harboring in its genome 2 copies of proviral DNA. These cells, which express HIV at low levels under normal growing conditions, increase HIV expression remarkably, after the addition of cytokines or other inducing agents. Different concentrations of conditioned medium from fetal glial cell lines SVG or POJ or from the adult glial cell line HJC were added to the culture of U1 cells. To monitor the level of HIV expression in the U1 cell line, reverse transcriptase activity was determined in the supernatant. The results showed that conditioned media from both fetal glial cell lines SVG and POJ induced the activation of HIV expression in U1 cells in a dose-dependent manner. In contrast, the conditioned medium from adult-derived glial cells did not induce HIV expression, even after stimulation with LPS, which has been described to induce in many cell types the synthesis of TNF alpha, a potent activator of HIV. These data suggest that fetal glial cells could stimulate the activation of HIV expression in brain-infiltrating monocytic cells during gestation, thereby contributing to the progression of HIV encephalopathy in children.

Keywords: Adult Cell Line Comparative Study Fetus Human HIV/*GROWTH & DEVELOPMENT/GENETICS Lipopolysaccharides/PHARMACOLOGY Neuroglia/*PHYSIOLOGY Proviruses/*GROWTH & DEVELOPMENT/GENETICS Tumor Necrosis Factor/BIOSYNTHESIS *Virus Activation ABSTRACT
910730
M9170997

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