THE OXYGEN FREE RADICAL-ASSOCIATED DNA ADDUCT 8-OXOGUANINE CAUSES G-C TO T-A TRANSVERSIONS DURING IN VIVO DNA REPLICATION (MEETING ABSTRACT) NLM AIDSLINE Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

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THE OXYGEN FREE RADICAL-ASSOCIATED DNA ADDUCT 8-OXOGUANINE CAUSES G-C TO T-A TRANSVERSIONS DURING IN VIVO DNA REPLICATION (MEETING ABSTRACT)

Proc Annu Meet Am Assoc Cancer Res; 32:A895 1991. Unique Identifier : AIDSLINE ICDB/91673722
Cheng KC; Cahill DS; Kasai H; Nishimura S; Loeb LA; Dept. of Pathology, Univ. of Washington, Seattle, WA 98195

Abstract: A major product of oxygen damage to DNA is 8-oxoguanine. The mutagenic potential of this adduct was tested in the M13G assay, a site-specific assay for in vivo mutations caused by guanine adducts inserted into the E coli lacZ gene. Site-specific insertion of 8-oxoguanine using the DNA polymerase activity of HIV-1 reverse transcriptase (which allows promiscuous addition of adducted nucleotides to DNA) was followed by transfection into E coli. The mutation frequency of the 8-oxoguanine-containing DNA was 0.11% (54,000 plaques screened), which was 4 times higher than controls. 40 of the 68 mutants were G-C to T-A transversions, whereas none of these mutations was found among the 9 mutants sequenced from controls (among 37,000 screened). Thus, the predominant single base substitutions caused by 8-oxoguanine are G-C to T-A transversions, suggesting that 8-oxoguanine has the potential to contribute to the occurrence of spontaneous G-C to T-A transversions.
Keywords: Base Composition DNA/CHEMISTRY/*GENETICS *DNA Damage Escherichia coli/GENETICS Free Radicals Guanine/*ANALOGS & DERIVATIVES/CHEMISTRY HIV-1/ENZYMOLOGY Mutagenesis, Site-Directed *Mutation *Oxygen RNA-Directed DNA Polymerase/GENETICS Transfection ABSTRACTKWDbasecompositiondna/chemistry/KWDgeneticsKWDdnadamageescherichiacoli/geneticsfreeradicalsguanine/KWDanalogs&derivatives/chemistryhiv-1/enzymologymutagenesis,site-directedKWDmutationKWDoxygenrna-directeddnapolymerase/geneticstransfectionabstract
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M9170994

Copyright © 1991 - National Library of Medicine. Reproduced under license with the National Library of Medicine, Bethesda, MD.

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