Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.
Viral and cellular gene expression in CD4 positive lymphoid cells infected by the simian immunodeficiency virus, SIV/Mne
Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:28 (abstract no. 12). Unique Identifier : AIDSLINE PRIM8/900012 Agy MB; Foy K; Gale MJ; Clark EA; Katze MG; Department of Microbiology and Regional Primate Research Center,; University of Washington, Seattle WA 98195
Abstract:
Our laboratory has undertaken an analysis of cellular and viral gene expression in human CD4 positive cells infected by the human and simian immunodeficiency viruses. The purpose of our current study was two-fold: (i) To examine the effects of SIV/Mne infection on cellular gene expression in susceptible human CD4 positive lymphoid cells and compare the results to those of HIV-1; and (ii) To investigate the molecular mechanisms responsible for the restriction of SIV/Mne replication in CD4 positive cells readily infected by HIV-1. Regarding aim (i), we determined that SIV infection does not impose selective blocks on cellular gene expression. These results are in marked contrast to those recently described for HIV-1 in which we found that host cell protein synthesis was selectively inhibited in HIV-1 infected cells as a result of cellular mRNA degradation (Agy et al., 1990 Virology 177:251-258). No such reduction in cellular mRNA stability or cellular protein synthesis was detected in SIV infected cells. Regarding aim (ii), further differences between SIV/Mne and HIV-1 were observed in a study of several human CD4 lymphoid cell lines including MT-4, C8166, CEMx174, Jurkat, CEM, and HUT 78 cells. While HIV-1 infected all these cell lines, SIV/Mne efficiently infected only MT-4, CEMx174, and C8166 cells. Repeated efforts to infect CEM or Jurkat cells were unsuccessful (as determined by negative PCR analysis) while HUT 78 cells supported a limited infection detectable only by PCR amplification of viral DNA. These data suggest the block in viral replication in the non-susceptible cell lines to be at an early step. Interestingly all the SIV susceptible cell lines were virally transformed, C8166 and MT-4 by HTLV-1, and CEMx174 by Epstein-Barr virus. Furthermore, MT-4 and C8166 cells express high levels of the interleukin 2 receptor (IL2 R) and unexpectedly, all the susceptible cells expressed the B cell surface marker, B7/BB1, on their cell surfaces. These observations, taken together, highlight differences between the HIV and SIV viruses, and suggest that for efficient replication, SIV/Mne may require additional cell surface molecules, cofactors provided by transforming viruses, or a complex interplay between the two.
Keywords: Animal Cell Line Cell Transformation, Viral Comparative Study CD4-Positive T-Lymphocytes/*METABOLISM/MICROBIOLOGY *Gene Expression Regulation Gene Expression Regulation, Viral Herpesvirus 4, Human/PHYSIOLOGY Human HIV-1/*GENETICS HTLV-I/GENETICS Macaca nemestrina Polymerase Chain Reaction Receptors, Interleukin-2/METABOLISM RNA, Messenger/METABOLISM SIV/GROWTH & DEVELOPMENT/*GENETICS *Virus Replication ABSTRACT 910730
M9170982
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Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.
