Induction of infection of rhesus monkeys with topical instillation of SIV/B670 into the conjunctival sac.

Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

Induction of infection of rhesus monkeys with topical instillation of SIV/B670 into the conjunctival sac.

Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:29 (abstract no. 13). Unique Identifier : AIDSLINE PRIM8/900013
Conway MD; Murphey-Corb M; Davison-Fairburn B; Martin L; Delta Regional Primate Research Center, Tulane University,; Covington, LA

Abstract: Laboratory accidents in which HIV is accidently instilled into the conjunctival cul de sac provide a means by which the retrovirus comes in contact with a mucous membrane and may provide a route for systemic infection. We tested the ability of SIV, a lentivirus immunologically and antigenically related to HIV, to induce local and systemic infection in 3 rhesus monkeys. Rhesus monkeys are readily infected with SIV and provide an animal model of immunodeficiency disease. One eye of each animal was exposed to cryopreserved SIV/Delta B670 by topical instillation into the conjunctival cul de sac. During the first month post-infection, animals were observed weekly for conjunctivitis or other signs of infection. Conjunctiva were swabbed for virus culture and biopsied for polymerase chain reaction (PCR) at 2 weeks, 4 weeks and monthly thereafter for 6 months. Additionally, blood samples were tested for evidence of SIV viremia by in vitro culture, antigenemia and lymphocyte subset analysis. At 1 and 2 months, viral DNA was detected in conjunctival tissues by PCR in 2 of 3 animals. Although conjunctivitis was seen, virus was not recovered in culture from swabs of conjunctiva. Evidence of systemic seroconversion was detected by ELISA in 1 of the animals but was inconclusive in 2 animals. PCR of peripheral blood lymphocytes is being investigated to confirm systemic infection in these animals.

Keywords: Animal Antigens, Viral/ANALYSIS *Conjunctivitis, Viral/COMPLICATIONS/GENETICS/PATHOLOGY Cryopreservation DNA, Viral/ANALYSIS Enzyme-Linked Immunosorbent Assay Lymphocyte Subsets/MICROBIOLOGY Macaca mulatta Polymerase Chain Reaction *Simian Acquired Immunodeficiency Syndrome/COMPLICATIONS/GENETICS/ PATHOLOGY SIV/*PHYSIOLOGY ABSTRACT
910730
M9170981

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