Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.
Interactions of rhesus cytomegalovirus (CMV) and simian immunodeficiency virus (SIV) in co-infected macaques.
Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:35 (abstract no. 19). Unique Identifier : AIDSLINE PRIM8/900019 Alcendor DJ; Vogel PJ; Martfeld DJ; Barry PA; Dandekar S; Luciw PA; Departments of Medical Pathology and Internal Medicine,; University of California, Davis, CA 95616
Abstract:
Infection of rhesus macaques with rhesus cytomegalovirus (CMV) is widespread at the California Primate Research Center (CPRC). As with infection with CMV in the majority of healthy humans, infection of healthy macaques with rhesus CMV appears to cause no clinical sequelae. However, macaques infected with the simian immunodeficiency virus (SIV) frequently present with CMV pathology in late stages of disease. We have chosen this animal model for basic investigations on CMV biology and to determine whether CMV can directly augment SIV-induced disease. CMV was isolated from an SIV-infected rhesus macaque at the CPRC and propagated in tissue culture cells. Using an immediate early (IE) probe from the African green monkey (AGM) CMV isolate, we cloned the cognate rhesus CMV IE gene. A portion of this gene has been sequenced; the promoter region and first non-coding exon share 70-85% sequence homology with the AGM CMV IE. Analysis of viral transcription in infected tissue culture cells revealed that the rhesus CMV IE gene is autoregulated. Transient expression systems demonstrate that the rhesus CMV IE gene transactivates the promoter in the long terminal repeat of SIV. We have initiated in situ hybridizations experiments utilizing lung samples from three SIV-infected animals. Tissues from two of the animals showed strong histological evidence of CMV infection (cytomegalic cells with intranuclear and intracytoplasmic inclusions). Lung tissue from the third animal lacked cytomegalic cells but contained multifocal intra-alveolar accumulations of neutrophils and diffuse interstitial edema. With probe from the rhesus CMV IE gene, viral nucleic acid was detected in tissues from all three animals; very high numbers of CMV-positive cells were detected in the localized lesions containing cytomegalic cells and viral inclusions. However, cells determined to be CMV-positive by in situ hybridization were far more numerous and widely disseminated than those identifiable histologically. Histiocytes, endothelial cells, occasional Type II pneumocytes and alveolar macrophages were shown to be infected. These results demonstrate that the rhesus macaque model is a useful model for the study of concurrent infections by SIV and CMV.
Keywords: Animal Antigens, Viral/GENETICS Cercopithecus aethiops Cytomegalovirus/*GENETICS/ISOLATION & PURIF Cytomegalovirus Infections/COMPLICATIONS/*GENETICS/PATHOLOGY Disease Models, Animal Endothelium/MICROBIOLOGY Gene Expression Regulation, Viral Genes, Viral Histiocytes/MICROBIOLOGY Macaca mulatta Macrophages/MICROBIOLOGY Promoter Regions (Genetics) Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Simian Acquired Immunodeficiency Syndrome/COMPLICATIONS/*GENETICS/ PATHOLOGY SIV/*GENETICS Tissue Culture ABSTRACT 910730
M9170975
AEGiS presents published material, reprinted with permission and neither endorses nor opposes any material. All information contained on this website, including information relating to health conditions, products, and treatments, is for informational purposes only. It is often presented in summary or aggregate form. It is not meant to be a substitute for the advice provided by your own physician or other medical professionals. Always discuss treatment options with a doctor who specializes in treating HIV.
Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.
