Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.
Vaccination against primate lentiviruses in cynomolgus monkeys.
Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:51 (abstract no. 35). Unique Identifier : AIDSLINE PRIM8/900035 Putkonen P; Thorstensson R; Biberfeld G; Department of Immunology, National Bacteriological Lab,; Stockholm, Sweden
Abstract:
The related SIVsm and HIV-2 can infect cynomolgus monkeys, but only SIV causes an immunosuppressive disease. At NBL two vaccine studies have been done. In the first experiment, three macaques were preinfected and immunized with live HIV-2 (SBL-K135) and 168 days later challenged with 10-100 animal ID50 of SIV to study protective immunity. The 3 animals vaccinated with live HIV-2 showed a restricted virus replication after SIV challenge but showed no CD4 cell decrease or lymphadenopathy after 13 months of follow-up. In contrast 4/4 SIV-infected controls became persistently infected, developed CD4 cell decrease and 2/2 died from AIDS within a year. Thus, vaccination with live HIV-2 did not induce sterilizing immunity but induced protection against immunosuppression and disease caused by a non-homologous but type-related virus strain (SIV). In the second experiment, we used inactivated HIV-2 to induce protection in macaques against a subsequent homologous HIV-2 challenge. 4 animals were immunized, 2 of them with 5 im injections of 100 or 300 mug viral protein in incomplete Freund's adjuvant (IFA) and the other 2 animals with 4 injections of 25 or 50 mug viral protein in iscoms. Challenge with 100 ID50 of live HIV-2 (SBL-6669) was done 2 weeks after the last booster. The two monkeys immunized with killed virus in IFA have shown no signs of infection after more than 7 months as determined by virus isolation, PCR and transfusion assay. Virus was isolated repeatedly after live HIV-2 challenge in all 4 non-immunized controls and in the 2 monkeys immunized with iscoms. Thus, whole killed HIV-2 vaccine in IFA induced isolate-specific immunity in macaques. In another experiment passive immunity against HIV-2 was studied. Ten ml of serum from one of the immunized and protected animals were injected iv into each of 4 monkeys 6 hours before challenge with 100 ID50 of HIV-2 (SBL-6669). 4/4 controls and 3/4 passively immunized animals became virus isolation positive 10-21 days postchallenge. Passive transfer of serum delayed the onset of HIV-2 viremia in one monkey.
Keywords: Acquired Immunodeficiency Syndrome/COMPLICATIONS/PREVENTION & CONTROL Animal CD4-Positive T-Lymphocytes/IMMUNOLOGY Dose-Response Relationship, Immunologic Freund's Adjuvant HIV-2/GROWTH & DEVELOPMENT/*IMMUNOLOGY Leukocyte Count Macaca fascicularis Polymerase Chain Reaction Simian Acquired Immunodeficiency Syndrome/COMPLICATIONS/ *PREVENTION & CONTROL SIV/GROWTH & DEVELOPMENT/*IMMUNOLOGY *Vaccination Vaccines, Inactivated Viral Vaccines Viremia/COMPLICATIONS *Virus Replication ABSTRACT 910730
M9170959
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Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.
