The induction of anti-simian retrovirus-1 (SRV-1) neutralizing antibodies by synthetic peptides and studies on the cell receptor to the virus.

Important note: Information in this article was accurate in 1991. The state of the art may have changed since the publication date.

The induction of anti-simian retrovirus-1 (SRV-1) neutralizing antibodies by synthetic peptides and studies on the cell receptor to the virus.

Symp Nonhum Primate Models AIDS. 1990 Nov 28-30;8:59 (abstract no. 43). Unique Identifier : AIDSLINE PRIM8/900043
Torres J; Werner L; Malley A; Benjamini E; Department of Medical Microbiology and Immunology, School of; Medicine, University of California, Davis

Abstract: We have recently demonstrated that rhesus monkey antibodies which neutralize SRV-1 are directed to an area encompassing residues 147-162 of the viral envelope protein. Although immunization of mice with the synthetic peptide-KLH conjugate induced antibodies which bind with the peptide, with the viral protein and with the whole virus, they were unable to neutralize, in vitro, viral infectivity of Raji cells. Further studies demonstrated that immunization of mice with a larger synthetic peptide consisting of residues 142-167 conjugated to several carrier proteins or polymerized with glutaraldehyde induced antibodies capable of binding with the viral envelope protein and with whole virus. Moreover, the antibodies induced by these immunogens were capable of specifically neutralizing, in vitro, infectivity by SRV-1 but not by SRV-2. Experiments are in progress to assess the efficacy of these immunogens in monkeys. The cell receptor for the virus was obtained following extraction of Raji cells with non-ionic detergents in the presence of protein inhibitors. The receptor was identified by radioimmunoprecipitation using native gp70 from purified SRV-1 virus. Several experiments suggest a receptor with a consistent molecular weight and binding characteristics. Work is in progress on the biochemical characterization of the receptor. The above findings indicate that the receptor-virus recognition is through residues 142-167 of the viral envelope protein and that the neutralizing antibodies exert their effect by inhibiting the virus-receptor interaction.

Keywords: Animal Antibodies, Viral/*BIOSYNTHESIS Cells, Cultured Haplorhini Molecular Weight Neutralization Tests Peptides/*PHARMACOLOGY Radioimmunoprecipitation Assay Receptors, Virus/*IMMUNOLOGY Retroviruses Type D, Simian/DRUG EFFECTS/*IMMUNOLOGY Vaccines, Synthetic Viral Envelope Proteins/IMMUNOLOGY ABSTRACT
910730
M9170951

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